Phage on the stage

Temple, Louise; Lewis, Lynn

doi:10.1080/21597081.2015.1062589

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…Spherical capsids with T = 7 symmetry have been enormously well studied in the last two decades, thanks to increased access to better technology within the field of cryo-electron microscopy (cryo-EM), which is ideally suited to image complex molecular machines [ 22 , 23 , 24 , 25 , 26 ]. A wealth of information regarding larger and more complex capsid geometries has also benefitted from current advances in environmental isolation methodology (“phage hunting”) [ 27 , 28 , 29 ] and access to better laboratory culture schemes, revealing an unprecedented breadth of capsid geometry. Overall, it has become evident that the ability of viral complexes to correctly assemble and function lies within specific conformations that promote relevant protein:protein interactions.…”

Section: Principles Of Icosahedral Virus Assemblymentioning

confidence: 99%

Breaking Symmetry in Viral Icosahedral Capsids as Seen through the Lenses of X-ray Crystallography and Cryo-Electron Microscopy

Parent

Schrad

Cingolani

2018

Viruses

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The majority of viruses on Earth form capsids built by multiple copies of one or more types of a coat protein arranged with 532 symmetry, generating an icosahedral shell. This highly repetitive structure is ideal to closely pack identical protein subunits and to enclose the nucleic acid genomes. However, the icosahedral capsid is not merely a passive cage but undergoes dynamic events to promote packaging, maturation and the transfer of the viral genome into the host. These essential processes are often mediated by proteinaceous complexes that interrupt the shell’s icosahedral symmetry, providing a gateway through the capsid. In this review, we take an inventory of molecular structures observed either internally, or at the 5-fold vertices of icosahedral DNA viruses that infect bacteria, archea and eukaryotes. Taking advantage of the recent revolution in cryo-electron microscopy (cryo-EM) and building upon a wealth of crystallographic structures of individual components, we review the design principles of non-icosahedral structural components that interrupt icosahedral symmetry and discuss how these macromolecules play vital roles in genome packaging, ejection and host receptor-binding.

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Section: Principles Of Icosahedral Virus Assemblymentioning

confidence: 99%

Breaking Symmetry in Viral Icosahedral Capsids as Seen through the Lenses of X-ray Crystallography and Cryo-Electron Microscopy

Parent

Schrad

Cingolani

2018

Viruses

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show abstract

“…Incorporating hands-on, active learning teaching methods at the secondary and undergraduate school levels increases student retention and performance across science, technology, engineering, and mathematics (STEM) disciplines [1][2][3]. In particular, biotechnology lab-based research, often done in high schools through inter-institutional joint studies such as the Wolbachia Project or SEA-PHAGES program [4,5] contributes to significant improvement in students' self-reported technical and professional skills [6,7].…”

Section: Introductionmentioning

confidence: 99%

At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring

Jung

Rasor

Rybnicky

et al. 2023

Preprint

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As the field of synthetic biology expands, the need to grow and train science, technology, engineering, and math (STEM) practitioners is essential. However, the lack of access to hands-on demonstrations has led to inequalities of opportunity and practice. In addition, there is a gap in providing content that enables students to make their own bioengineered systems. To address these challenges, we develop four shelf-stable cell-free biosensing educational modules that work by just-adding-water and DNA to freeze-dried crude extracts of Escherichia coli. We introduce activities and supporting curricula to teach the structure and function of the lac operon, dose-responsive behavior, considerations for biosensor outputs, and a 'build-your-own' activity for monitoring environmental contaminants in water. We piloted these modules with K-12 teachers and 130 high school students in their classrooms (and at home) without professional laboratory equipment or researcher oversight. This work promises to catalyze access to interactive synthetic biology education opportunities.

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“…Incorporating hands-on, active learning teaching methods at the secondary and undergraduate school levels increases student retention and performance across science, technology, engineering, and mathematics (STEM) disciplines. 1−3 In particular, biotechnology lab-based research, often done in high schools through inter-institutional joint studies such as the Wolbachia Project or SEA-PHAGES program, 4,5 contributes to significant improvement in students' self-reported technical and professional skills. 6,7 Educational laboratory modules for synthetic biology, the fundamental science and engineering research that engineers biology to tackle global challenges (e.g., access to medicines, sustainable manufacturing), 8,9 are in demand.…”

Section: ■ Introductionmentioning

confidence: 99%

At-Home, Cell-Free Synthetic Biology Education Modules for Transcriptional Regulation and Environmental Water Quality Monitoring

Jung,

Rasor,

Rybnicky

et al. 2023

ACS Synth. Biol.

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As the field of synthetic biology expands, the need to grow and train science, technology, engineering, and math (STEM) practitioners is essential. However, the lack of access to hands-on demonstrations has led to inequalities of opportunity and practice. In addition, there is a gap in providing content that enables students to make their own bioengineered systems. To address these challenges, we develop four shelf-stable cell-free biosensing educational modules that work by simply adding water and DNA to freeze-dried crude extracts of non-pathogenic Escherichia coli. We introduce activities and supporting curricula to teach the structure and function of the lac operon, dose-responsive behavior, considerations for biosensor outputs, and a “build-your-own” activity for monitoring environmental contaminants in water. We piloted these modules with K-12 teachers and 130 high-school students in their classroomsand at homewithout professional laboratory equipment. This work promises to catalyze access to interactive synthetic biology education opportunities.

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Phage on the stage

Cited by 3 publications

References 33 publications

Breaking Symmetry in Viral Icosahedral Capsids as Seen through the Lenses of X-ray Crystallography and Cryo-Electron Microscopy

Breaking Symmetry in Viral Icosahedral Capsids as Seen through the Lenses of X-ray Crystallography and Cryo-Electron Microscopy

At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring

At-Home, Cell-Free Synthetic Biology Education Modules for Transcriptional Regulation and Environmental Water Quality Monitoring

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