Phage-mediated Dispersal of Biofilm and Distribution of Bacterial Virulence Genes Is Induced by Quorum Sensing

Rossmann, Friederike S.; Raček, Tomáš; Wobser, Dominique; Puchałka, Jacek; Rabener, Elaine M.; Reiger, Matthias; Hendrickx, Antoni P. A.; Diederich, Ann Kristin; Jung, Kirsten; Klein, Christoph; Hüebner, Johannes

doi:10.1371/journal.ppat.1004653

Cited by 82 publications

(76 citation statements)

References 41 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, diverse mobile elements coordinate their dissemination via QS, including AHL-based control of conjugative Ti plasmid transfer in Agrobacterium tumefaciens (Fuqua and Winans, 1994). Furthermore, many QS signals, including AHLs, Pseudomonas quinolone signal (PQS), and AI-2, can induce prophage induction in Gram-positive and Gram-negative bacteria (Fernández-Piñar et al., 2011, Hargreaves et al., 2014, Rossmann et al., 2015). It is salient that we observed QS-dependent regulation of type III-A activity.…”

Section: Discussionmentioning

confidence: 99%

Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems

et al. 2016

View full text Add to dashboard Cite

SummaryBacteria commonly exist in high cell density populations, making them prone to viral predation and horizontal gene transfer (HGT) through transformation and conjugation. To combat these invaders, bacteria possess an arsenal of defenses, such as CRISPR-Cas adaptive immunity. Many bacterial populations coordinate their behavior as cell density increases, using quorum sensing (QS) signaling. In this study, we demonstrate that QS regulation results in increased expression of the type I-E, I-F, and III-A CRISPR-Cas systems in Serratia cells in high-density populations. Strains unable to communicate via QS were less effective at defending against invaders targeted by any of the three CRISPR-Cas systems. Additionally, the acquisition of immunity by the type I-E and I-F systems was impaired in the absence of QS signaling. We propose that bacteria can use chemical communication to modulate the balance between community-level defense requirements in high cell density populations and host fitness costs of basal CRISPR-Cas activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The involved bacteriophage is closely related to the filamentous phage Pf1 and exists as a prophage within the bacterial genome (Webb et al ., ). A biofilm dispersal process linked to prophage induction was also observed in Enterococcus faecalis (Rossmann et al ., ) (Table ). Rossmann et al .…”

Section: Molecular Mechanisms Governing Biofilm Dispersalmentioning

confidence: 97%

“…Rossmann et al showed that the AI-2 universal QS molecule is a strong inducer of prophages in E. faecalis V583DABC. Exogenous addition of AI-2 up-regulates the genes of prophage 5 (pp5) that are involved in lysis (i.e., endolysin, holing) resulting either in a significant reduction in biofilm formation by E. faecalis or in a dispersal of an already formed biofilm (Rossmann et al, 2015). However, the underlying mechanism of dispersal through phage release is still not elucidated.…”

Section: Effectors Promoting Biofilm Dispersalmentioning

confidence: 99%

Biofilm dispersal: multiple elaborate strategies for dissemination of bacteria with unique properties

Guilhen

Forestier

Balestrino

2017

Molecular Microbiology

174

143

View full text Add to dashboard Cite

Summary In most environments, microorganisms evolve in a sessile mode of growth, designated as biofilm, which is characterized by cells embedded in a self‐produced extracellular matrix. Although a biofilm is commonly described as a “cozy house” where resident bacteria are protected from aggression, bacteria are able to break their biofilm bonds and escape to colonize new environments. This regulated process is observed in a wide variety of species; it is referred to as biofilm dispersal, and is triggered in response to various environmental and biological signals. The first part of this review reports the main regulatory mechanisms and effectors involved in biofilm dispersal. There is some evidence that dispersal is a necessary step between the persistence of bacteria inside biofilm and their dissemination. In the second part, an overview of the main methods used so far to study the dispersal process and to harvest dispersed bacteria was provided. Then focus was on the properties of the biofilm‐dispersed bacteria and the fundamental role of the dispersal process in pathogen dissemination within a host organism. In light of the current body of knowledge, it was suggested that dispersal acts as a potent means of disseminating bacteria with enhanced colonization properties in the surrounding environment.

show abstract

“…Besides spreading virulence and antibiotic resistance markers among bacteria, bacteriophages can promote the expression of virulence/resistance traits of the infected cell. For instance, a polylysogenic Enterococcus faecalis strain displayed greater virulence than the prophage-free isogenic strain in sepsis and endocarditis animal models [39]. Similarly, temperate phage Pf4 participates in the pathogenicity of its host, P. aeruginosa, as shown in a mouse infection model [40].…”

Section: Virulence and Antibiotic Resistancementioning

confidence: 99%

Phage or foe: an insight into the impact of viral predation on microbial communities

2018

View full text Add to dashboard Cite

Since their discovery, bacteriophages have been traditionally regarded as the natural enemies of bacteria. However, recent advances in molecular biology techniques, especially data from "omics" analyses, have revealed that the interplay between bacterial viruses and their hosts is far more intricate than initially thought. On the one hand, we have become more aware of the impact of viral predation on the composition and genetic makeup of microbial communities thanks to genomic and metagenomic approaches. Moreover, data obtained from transcriptomic, proteomic, and metabolomic studies have shown that responses to phage predation are complex and diverse, varying greatly depending on the bacterial host, phage, and multiplicity of infection. Interestingly, phage exposure may alter different phenotypes, including virulence and biofilm formation. The complexity of the interactions between microbes and their viral predators is also evidenced by the link between quorum-sensing signaling pathways and bacteriophage resistance. Overall, new data increasingly suggests that both temperate and virulent phages have a positive effect on the evolution and adaptation of microbial populations. From this perspective, further research is still necessary to fully understand the interactions between phage and host under conditions that allow co-existence of both populations, reflecting more accurately the dynamics in natural microbial communities.

show abstract

Phage-mediated Dispersal of Biofilm and Distribution of Bacterial Virulence Genes Is Induced by Quorum Sensing

Cited by 82 publications

References 41 publications

Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems

Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems

Biofilm dispersal: multiple elaborate strategies for dissemination of bacteria with unique properties

Phage or foe: an insight into the impact of viral predation on microbial communities

Contact Info

Product

Resources

About