Phage Display Technology and its Applications in Cancer Immunotherapy

Abstract

Background: Phage display is an effective technology for generation and selection targeting protein for a variety of purpose, which is based on a direct linkage between the displayed protein and the DNA sequence encoding it and utilized in selecting peptides, improving peptides affinity and indicating protein-protein interactions. Phage particles displaying peptide have the potential to apply in the identification of cell-specific targeting molecules, identification of cancer cell surface biomarkers, identification anti-cancer peptide, and the design of peptide-based anticancer therapy. Method/Results: Literature searches, reviews and assessments about Phage were performed in this review from PubMed and Medline databases. Conclusion: The phage display technology is an inexpensive method for expressing exogenous peptides, generating unique peptides that bind any given target and investigating protein-protein interactions. Due to the powerful ability to insert exogenous gene and display exogenous peptides on the surface, phages may represent a powerful peptide delivery system that can be utilized to develop rapid, efficient, safe and inexpensive cancer therapy methods.

Enhancement of T cell infiltration via tumor-targeted Th9 cell delivery improves the efficacy of antitumor immunotherapy of solid tumors

“…Therefore, a specific ligand targeting osteosarcoma is of great significance to solve the current dilemma of osteosarcoma immunotherapy. Phage display biopanning technology utilizes a phage library expressing billions of unique polypeptides to screen the target-binding peptides based on direct interaction between displayed peptides and unknown targets ([ 44 , 45 ]). The high flexibility, efficiency, and safety of phage display enable its broad application in identifying antibodies and cancer cell targeting molecules.…”

An auto-antibody identified from phenotypic directed screening platform shows host immunity against EV-A71 infection

“…There are several platforms which display antibody or scFv libraries to screen for the candidates, and phage display is the most powerful and applicable one based on the binding characteristics between target and candidate [13,14]. It is applied to develop antibody candidates against not only infectious agents like bacteria, viruses, and parasites, but also diagnostic or therapeutic targets or molecules [15][16][17][18][19][20][21]. In addition, intracellular library display system had been developed and applied to screen the candidates through cellular phenotypic activity, such as survival from virus challenge [22][23][24].…”

Phage Display Technology as a Powerful Platform for Antibody Drug Discovery

“…Therefore, the development of phage display technology provides optimal sequences to target peptides or proteins, unlike conventional alanine scanning and other methods, and aids understanding of their molecular evolution [2][3][4][5][6][7]. Although various kinds of molecular display technologies such as ribosome display [8,9], and yeast display technologies [10,11] have been proposed, phage display technology is frequently employed because of a high diversity of molecules that can be displayed and ease of handling [12,13].…”