Phage Display Selection of an Anti-Idiotype-Antibody with Broad-Specificity to Deoxynivalenol Mycotoxins

Abstract

The use of synthetic antibody libraries and phage displays provides an efficient and robust method for the generation of antibodies against a wide range of targets with highly specific binding properties. As the in vitro selection conditions can be easily controlled, these methods enable the rapid generation of binders against difficult targets such as toxins and haptens. In this study, we used deoxynivalenol mycotoxin as a target to generate anti-idiotype-antibodies with unique binding properties from synthetic antibody libraries. The binding of the selected anti-idiotype antibodies can be efficiently inhibited with the addition of free isoforms of deoxynivalenol. The antibody was consecutively used to develop deoxynivalenol-specific ELISA and TRF-immunoassays, which can detect deoxynivalenol and two of the most common metabolic isoforms in the range of 78–115 ng/mL.

Nanobodies Selectively Binding to the Idiotype of a Dengue Virus Neutralizing Antibody Do Not Necessarily Mimic the Viral Epitope

“…Our work confirms that in vitro selection of nanobodies performed imposing stringent parameters represents a valuable modality to isolate binders specific for particular domains, such as antigen epitopes and Ab paratopes [ 34 , 35 ]. We obtained a large and structurally highly heterogeneous array of clones with specificity for a specific idiotype.…”

A Novel Cost-Effective Nanobody against Fumonisin B1 Contaminations: Efficacy Test in Dairy Milk and Chickens

“…Nanobodies termed as VHH antibody are usually isolated from the library constructed by primary antibody immunization and eluted with the free antigen. Although the nanobodies are a particularly useful tool for monitoring mycotoxins in food and feedstuffs, as they are easily genetic engineered and have superior stability [31], the potential removal of mycotoxin has not been fully investigated. Nanobodies are reported to recognize the active site of the antigen, and also serve as a surrogate for the original antigen and compete with the original antigen for the primary antibody [32].…”

TSAT: Efficient evaluation software for NGS data of phage/mirror-image phage display selections

“…Classical phage display ( 1 , 2 ) or mirror-image phage display ( 3 ) selections may yield peptides, antibodies, or other biomolecules that are highly specific to a target after several rounds of selection. These techniques were used successfully in the identification of binders for potential disease-causing proteins such as Aβ ( 4 ) or certain types of cancer ( 5 , 6 , 7 , 8 , 9 ) and are widely used for antibody generation ( 10 , 11 , 12 ) and in different disciplines, such as material science ( 13 ). The number of selection rounds required may vary depending on the target used.…”