Phage-Based Assay for the Detection of Salmonella in Brazilian Poultry Products

Aquino, Nathanyelle Soraya Martins de; Elias, Susana de Oliveira; Gomes, Leonardo Vaz Alves; Tondo, Eduardo César

doi:10.26502/jfsnr.2642-11000077

Cited by 2 publications

(2 citation statements)

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“…Salmonella, a significant foodborne pathogen, is responsible for a staggering 153 million cases of gastroenteritis and approximately 57,000 deaths each year on a global scale [ 78 ]. Given the gravity of its impact, accurate and sensitive detection methods for Salmonella are paramount.…”

Section: Salmonellamentioning

confidence: 99%

Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics

Wahid,

Tiwana

2024

Future Microbiology

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Bacteriophages, as abundant and specific agents, hold significant promise as a solution to combat the growing threat of antimicrobial resistance. Their unique ability to selectively lyse bacterial cells without harming humans makes them a compelling alternative to traditional antibiotics and point-of-care diagnostics. The article reviews the current landscape of diagnostic technologies, identify gaps and highlight emerging possibilities demonstrates a comprehensive approach to advancing clinical diagnosis of microbial pathogens and covers an overview of existing phage-based bioassays. Overall, the provided data in this review effectively communicates the potential of bacteriophages in transforming therapeutic and diagnostic paradigms, offering a holistic perspective on the benefits and opportunities they present in combating microbial infections and enhancing public health.

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Section: Salmonellamentioning

confidence: 99%

Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics

Wahid,

Tiwana

2024

Future Microbiology

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“…To address this concern, a phage-based diagnostic method, known as the PhageDx™ Salmonella assay, was developed to facilitate Salmonella detection in food matrices [ 12 , 21 ]. Over the course of several studies, the PhageDx™ Salmonella assay has demonstrated excellent performance in both broth culture and across multiple food matrices, including ground turkey, powdered infant formula, hydroponic curly lettuce, and Brazilian poultry products [ 12 , 21 , 22 , 23 ]. The detection of contamination by this method is mediated by NanoLuc ® -encoding Salmonella reporter phages, engineered from two natural phages isolated from wastewater [ 12 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a Replication-Deficient Bacteriophage Reporter Lacking an Essential Baseplate Wedge Subunit

Gil,

Paulson,

Zahn

et al. 2023

Viruses

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Engineered bacteriophages (phages) can be effective diagnostic reporters for detecting a variety of bacterial pathogens. Although a promising biotechnology, the large-scale use of these reporters may result in the unintentional release of genetically modified viruses. In order to limit the potential environmental impact, the ability of these phages to propagate outside the laboratory was targeted. The phage SEA1 has been previously engineered to facilitate food safety as an accurate and sensitive reporter for Salmonella contamination. In this study, homologous recombination was used to replace the expression of an essential baseplate wedge subunit (gp141) in SEA1 with a luciferase, NanoLuc®. This reporter, referred to as SEA1Δgp141.NL, demonstrated a loss of plaque formation and a failure to increase in titer following infection of Salmonella. SEA1Δgp141.NL was thus incapable of producing infectious progeny in the absence of gp141. In contrast, production of high titer stocks was possible when gp141 was artificially supplied in trans during infection. As a reporter, SEA1Δgp141.NL facilitated rapid, sensitive, and robust detection of Salmonella despite an inability to replicate. These results suggest that replication-deficient reporter phages are an effective method to obtain improved containment without sacrificing significant performance or the ease of production associated with many phage-based diagnostic methods.

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Phage-Based Assay for the Detection of Salmonella in Brazilian Poultry Products

Cited by 2 publications

References 0 publications

Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics

Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics

Development of a Replication-Deficient Bacteriophage Reporter Lacking an Essential Baseplate Wedge Subunit

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