Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy

Abstract

Phage display technology (PDT), a combinatorial screening approach, provides a molecular diversity tool for creating libraries of peptides/proteins and discovery of new recombinant therapeutics. Expression of proteins such as monoclonal antibodies (mAbs) on the surface of filamentous phage can permit the selection of high affinity and specificity therapeutic mAbs against virtually any target antigen. Using a number of diverse selection platforms (e.g. solid phase, solution phase, whole cell and in vivo biopannings), phage antibody libraries (PALs) from the start point provides great potential for the isolation of functional mAb fragments with diagnostic and/or therapeutic purposes. Given the pivotal role of PDT in the discovery of novel therapeutic/diagnostic mAbs, in the current review, we provide an overview on PALs and discuss their impact in the advancement of engineered mAbs.

Targeted therapy of solid tumors by monoclonal antibody specific to epidermal growth factor receptor

“…In short, given that the selection of mAbs against EGFR with high specificity and anti-tumor activity, yet with low cost, is a challenging issue, we envision that this approach may be considered as an attractive alternative strategy to the one that use other technologies such as phage display for the development of mAbs [22,[43][44][45][46]. Based on our findings, the isolated mAb BF4 showed high specificity to the EGFRpositive A431 cells, but not the EGFR-negative CHO cells.…”

“…The success of this approach, however, is largely dependent upon the specificity and potency of mAbs to the designated antigens [18]. Various technologic have been exploited for optimization of the specificity and potency of antibodies (Abs), including chimerization and humanization of murine Abs, as well as reshaping and redesigning, conjugation and widening of the specificity of the mAb [19][20][21][22]. The optimized screening and selection method is believed to be one of the simple and cost-effective applicable approaches for the generation of high specific mAbs.…”

Pleiotropic cytotoxicity of VacA toxin in host cells and its impact on immunotherapy

“…New approaches for production of antibodies, such as phage display technique, 125,126 have been developed in the recent decades that provide a convenient and economic procedure for production of monoclonal antibodies. To the best of our knowledge, we are among the first teams who work on the development of mAb fragments by phage display against VacA toxin.…”

Phage display-based on-slide selection of tumor-specific antibodies on formalin-fixed paraffin-embedded human tissue biopsies

“…More than 30 antibodies and antibody conjugates have been approved for use in humans, and many more are undergoing clinical development [1]. Phage antibody display is currently the most efficient in vitro method for the selection of tumor-specific recombinant human antibodies [2]. Standard phage display selection procedures include panning against recombinant proteins, but suitable targets are often membrane proteins with multiple disulfide bonds, and such proteins are difficult to produce in bacteria because the expression yield is low and they do not fold efficiently [3].…”