Phage antibodies against heat shock proteins as tools for in vitro cancer diagnosis

Староверов, С. А.; Козлов, С. В.; Бровко, Ф. А.; Fursova, Ksenia; Shardin, Vitaly V.; Фомин, А. С.; Габалов, К. П.; Soldatov, Dmitry; Zhnichkova, Elena G.; Дыкман, Л. А.; Guliy, О. I.

doi:10.1016/j.biosx.2022.100211

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…HS can cause protein denaturation and aggregation, resulting in unbalanced protein homeostasis damaging the body, whereas HSPs can reverse or inhibit the expansion or denaturation of proteins under cell stress or high temperatures to protect the body 27 . Previous studies have demonstrated that HS can induce HSPs and nuclear transcription factors, resulting in the increased expression of HSP70/90 in chickens 28 .…”

Section: Discussionmentioning

confidence: 99%

Comparison study of protective effects of porcine bile acids and sheep bile acids against heat stress in chickens

Liu

Wang

et al. 2023

J Sci Food Agric

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BACKGROUND Heat stress (HS) is known to exert negative effects on the poultry and breeding industry, resulting in severe economic losses. Bile acids (BAs), an important component of bile, play a crucial role in improving the production performance of livestock and poultry, alleviating stress injury, and ensuring the health of livestock and poultry. At present, porcine BAs are widely used because of their therapeutic effects on HS; however, it remains unclear whether the same effects are exerted by sheep BAs, which are different from porcine BAs and have different compositions. In this study, we compared the anti‐HS effects of porcine BAs and sheep BAs in the diet by establishing an HS model of chicks and investigating the chicken performance, HS‐related genes' expression, oxidative stress markers, jejunal histoarchitecture, inflammatory cytokines' expression, jejunal secreted immunoglobulin A concentration, and cecal bacterial flora. RESULTS The results showed that the addition of sheep BAs to the diet increased the average daily weight gain and the feed conversion ratio of chicks. Under HS, sheep BAs were more effective than porcine BAs in improving the activities of lactate dehydrogenase and glutamic pyruvic transaminase in serum and the content/activity of malondialdehyde, superoxide dismutase, and reduced glutathione in serum and tissue, in reducing the messenger RNA (mRNA) expression of heat shock proteins (HSP60, HSP70, and HSP90) in the liver and jejunum, and in improving the histological structure and the expression of tight junction proteins (occludin and zonula occludens‐1) and enriching intestinal bacterial flora. However, porcine BAs were significantly inferior to sheep BAs in reducing the mRNA expression of inflammatory factors (interleukin‐6, interleukin‐1β, and tumor necrosis factor‐α). CONCLUSION The effect of sheep BAs was more significant than porcine BAs was in alleviating HS injury in chicks, suggesting that sheep BAs have great potential as new feed nutrition and health additive to improve poultry production performance and prevent HS. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Comparison study of protective effects of porcine bile acids and sheep bile acids against heat stress in chickens

Liu

Wang

et al. 2023

J Sci Food Agric

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“…It also propels the progress of diagnostic techniques and methods for monitoring patient responses throughout and post-treatment. Staroverov et al [ 145 ] developed Heat Shock Protein (HSP)-specific antibodies using a human scFv phage library. Levels of HSPs in affected animals’ blood sera were evaluated through immunoassays with recombinant Phage Display-derived antibodies [ 145 ].…”

Section: Phage Display To Select Antibodies Against Cancermentioning

confidence: 99%

“…Staroverov et al [ 145 ] developed Heat Shock Protein (HSP)-specific antibodies using a human scFv phage library. Levels of HSPs in affected animals’ blood sera were evaluated through immunoassays with recombinant Phage Display-derived antibodies [ 145 ]. HSPs are produced in response to stressors such as heat and toxins.…”

Section: Phage Display To Select Antibodies Against Cancermentioning

confidence: 99%

Progress on Phage Display Technology: Tailoring Antibodies for Cancer Immunotherapy

França,

Studart,

Bezerra

et al. 2023

Viruses

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The search for innovative anti-cancer drugs remains a challenge. Over the past three decades, antibodies have emerged as an essential asset in successful cancer therapy. The major obstacle in developing anti-cancer antibodies is the need for non-immunogenic antibodies against human antigens. This unique requirement highlights a disadvantage to using traditional hybridoma technology and thus demands alternative approaches, such as humanizing murine monoclonal antibodies. To overcome these hurdles, human monoclonal antibodies can be obtained directly from Phage Display libraries, a groundbreaking tool for antibody selection. These libraries consist of genetically engineered viruses, or phages, which can exhibit antibody fragments, such as scFv or Fab on their capsid. This innovation allows the in vitro selection of novel molecules directed towards cancer antigens. As foreseen when Phage Display was first described, nowadays, several Phage Display-derived antibodies have entered clinical settings or are undergoing clinical evaluation. This comprehensive review unveils the remarkable progress in this field and the possibilities of using clever strategies for phage selection and tailoring the refinement of antibodies aimed at increasingly specific targets. Moreover, the use of selected antibodies in cutting-edge formats is discussed, such as CAR (chimeric antigen receptor) in CAR T-cell therapy or ADC (antibody drug conjugate), amplifying the spectrum of potential therapeutic avenues.

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“…Especially in the time of vigorously promoting graded treatment and third party testing, low-cost, high-efficiency POCT products are more attractive for primary health care institutions and third party testing institutions. At present, the overall POCT market size is not large, but the development is fast; with the implementation of the graded treatment policy, the POCT market will develop rapidly, and the first layout of the enterprise with the policy and cost advantages will also usher in further development [90].…”

Section: Poctmentioning

confidence: 99%

Methods and Advances in the Design, Testing and Development of In Vitro Diagnostic Instruments

Wang

et al. 2023

Processes

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With the continuous improvement of medical testing and instrumentation engineering technologies, the design, testing and development methods of in vitro diagnostic instruments are developing rapidly. In vitro diagnostic instruments are also gradually developing into a class of typical high-end medical equipment. The design of in vitro diagnostic instruments involves a variety of medical diagnostic methods and biochemical, physical and other related technologies, and its development process involves complex system engineering. This paper systematically organizes and summarizes the design, testing and development methods of in vitro diagnostic instruments and their development in recent years, focusing on summarizing the related technologies and core aspects of the R&D process, and analyzes the development trend of the in vitro diagnostic instrument market.

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Phage antibodies against heat shock proteins as tools for in vitro cancer diagnosis

Cited by 8 publications

References 39 publications

Comparison study of protective effects of porcine bile acids and sheep bile acids against heat stress in chickens

Comparison study of protective effects of porcine bile acids and sheep bile acids against heat stress in chickens

Progress on Phage Display Technology: Tailoring Antibodies for Cancer Immunotherapy

Methods and Advances in the Design, Testing and Development of In Vitro Diagnostic Instruments

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