Phaeosaccardinula coffeicola and Trichomerium chiangmaiensis, two new species of Chaetothyriales (Eurotiomycetes) from Thailand

Abstract

Two species of sooty mould-like taxa, were obtained from living leaves of Coffea arabica plants collected in Chiang Mai Province, Thailand. Differences in phenotypic and phylogenetic characteristics based on combined large subunit nuclear ribosomal DNA and internal transcribed spacer sequences indicated that the two isolates represent two novel species (Phaeosaccardinula coffeicola and Trichomerium chiangmaiensis) within the order Chaetothyriales. Phaeosaccardinula coffeicola (Chaetothyriaceae) is distinguished morphologically from related species by its smaller asci and ascospores. Trichomerium chiangmaiensis (Trichomeriaceae) is morphologically distinguishable from its phylogenetically related species by its smaller conidial arms. Detailed taxonomic descriptions and illustrations of the new species are provided.

Additions to Chaetothyriaceae (Chaetothyriales): Longihyalospora gen. nov. and Ceramothyrium longivolcaniforme, a new host record from decaying leaves of Ficus ampelas

“…The matrix had 1040 distinct alignment patterns, with 37.84 % of undetermined characters or gaps. All analyses (ML, MP and BYPP) gave similar results and in agreement with previous studies based on multi-gene analyses (Zeng et al 2016; Maharachchikumbura et al 2018).…”

“…The phylogeny recovered herein also agrees with previously established ones in that Ceramothyrium is within the Chaetothyriales (Zeng et al 2016; Maharachchikumbura et al 2018; Yang et al 2018). Our new collection (MFLUCC19-0252) grouped in a well-supported clade (80% ML, 100% MP and 0.92 BYPP) with other Ceramothyrium species (Figure 1).…”

“…Chaetothyriaceae species are widespread in tropical and temperate regions (Hofmann and Piepenbring 2006; Chomnunti et al 2011, 2014; Hongsanan et al 2015; Zeng et al 2016; Maharachchikumbura et al 2018; Yang et al 2018; Farr and Rossman 2019). Wijayawardene et al (2018) accepted 16 genera in Chaetothyriaceae, but currently only seven genera ( Aphanophora , Camptophora , Ceramothyrium , Chaetothyrium , Cyphellophoriella , Phaeosaccardinula and Vonarxia ) have DNA sequence data.…”

“…Newly generated sequences were subjected to a standard BLAST search of GenBank to aid in phylogenetic taxon sampling. Other sequences used in the analyses (Table 1) were obtained from GenBank based on recently published data (Zeng et al 2016; Maharachchikumbura et al 2018; Yang et al 2018). The multiple alignments were made with MAFFT v. 7 at the web server (http://mafft.cbrc.jp/alignment/server), using default settings (Katoh and Standley 2013).…”

“…Due to some morphological similarities (i.e. bitunicate asci), Eriksson (1982) referred this family to the order Dothideales in Dothideomycetes, but subsequently, taxonomic studies have established its placement in Eurotiomycetes with support of molecular data (Chomnunti et al 2012, 2014; Tian et al 2014; Crous et al 2015; Maharachchikumbura et al 2018; Yang et al 2018). Currently, 16 genera are accepted in Chaetothyriaceae, viz.…”