pH-tunable bioelectrocatalysis based on layer-by-layer films assembled with TiO2 nanoparticles and poly(allylamine hydrochloride)

Miao, Xin; Hu, Naifei

doi:10.1016/j.jelechem.2011.06.015

Cited by 7 publications

(3 citation statements)

References 52 publications

(76 reference statements)

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“…Then, the clean QCM gold electrodes were dipped into a 4 mM MPS ethanol solution for 24 h to chemisorb an MPS monolayer on gold surface and introduce negative charges on gold surface. 15 At last, the {AMPC/GOx} n films were assembled on the Au/MPS surface in the same manner as on the PG electrodes.…”

Section: Methodsmentioning

confidence: 99%

“…[12][13][14] For example, in one of our previous studies, {PAH/TiO 2 } n LbL films assembled on electrodes with oppositely charged TiO 2 nanoparticles and polyelectrolyte poly (allylamine hydrochloride) (PAH) under appropriate pH conditions showed a pH-triggered bioelectrocatalysis reduction of H 2 O 2 by horseradish peroxidase (HRP) in solution with Fe(CN) 6 3− as a mediator through the switching of the environment pH. 15 Clays are layered phyllosilicate with ion-exchange properties, and exfoliated clay particles have a platelet shape with nanoscale size. Clay films are also inexpensive and demonstrate appreciable intercalation properties, ordered structure, and high stability.…”

mentioning

confidence: 99%

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Switchable Bioelectrocatalysis of Glucose Oxidase Immobilized into Multilayers with Lamellar Nanoparticles of Amino-Functionalized Magnesium Phyllosilicate Clay

Yao

Xiao

Yin

et al. 2021

J. Electrochem. Soc.

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Lamellar organic clay consisting of amino-functionalized magnesium phyllosilicate (AMPC) with controllable positive charges was successfully prepared by simple one-pot synthesis. Then, the positively charged AMPC was assembled layer by layer with negatively charged glucose oxidase (GOx) enzyme into {AMPC/GOx} n multilayer films on pyrolytic graphite (PG) electrode surface, where n is the number of the bilayers. The {AMPC/GOx} n film electrodes demonstrated sensitive and reversible pH-responsive switchable behavior toward the cyclic voltammetric (CV) responses of the ferrocenedicarboxylic acid (Fc(COOH)2) probe in solution. The CV peak currents were strongly enhanced at pH 5.0 and were significantly reduced at pH 9.0. The pH-sensitive behavior of the system originated from the electrostatic interactions between the nanolayered films and Fc(COOH)2 in solution. This property was applied to realize pH-triggered electrocatalytically oxidation of glucose by GOx enzyme in the {AMPC/GOx} n films. This model system demonstrates the great potential perspective of the fabrication of novel multiple factor-switchable biosensors with immobilized enzymes.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Switchable Bioelectrocatalysis of Glucose Oxidase Immobilized into Multilayers with Lamellar Nanoparticles of Amino-Functionalized Magnesium Phyllosilicate Clay

Yao

Xiao

Yin

et al. 2021

J. Electrochem. Soc.

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show abstract

“…For this researching objective, one of the major challenges is to realize the controllable or stimuli-responsive bioelectrocatalysis [2]. Among various external stimuli such as pH [1], ionic strength [3], temperature [4], and electric field [5], pH has been frequently used due to the simplicity and accuracy [1,6].…”

Section: Introductionmentioning

confidence: 99%

pH-Controllable Bioelectrocatalysis Based on Surface Charge and Conformation Switching of Nonelectroactive Protein

Wu¹

2018

International Journal of Electrochemical Science

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Lysozyme (Lys), a nonelectroactive protein, was covalently bonded with L-cysteine (L-Cys) selfassembled monolayers (SAMs) on Au electrode to obtain the pH-switchable interface (Lys/L-Cys). Based on the surface charge and conformational switching of Lys at pH 6.19 and 12.13, the Lys/LCys/Au electrode exhibited a pH-sensitive onoff behavior toward the electroactive probe of Fe(CN) 6 3 . As pH was lower or higher than the isoelectronic point (pI 11.0) of Lys, Lys on electrode was positively or negatively charged by the protonation or deprotonation of amino acid residues. Furthermore, protein conformation was reversibly folded or unfolded to the native state and unfolded intermediate accordingly. Consequently, the electrostatic force between Lys and probe of Fe(CN) 6 3 and reversible conformational change of Lys are responsible for the pH-sensitive onoff behavior of Lys/L-Cys/Au interface. Moreover, the switchable properties of Lys/L-Cys/Au electrode were used to realize pH-controlled electrochemical reduction of H 2 O 2 and exhibited high sensitivity for the bioelectrocatalytic reduction of H 2 O 2 . In the linear range of 0.211.2 mM, the response sensitivity was calculated to be 379 A mM 1 cm 2 . The investigations in the mechanism for pH-sensitive behavior and electrocatalysis of the interface suggest that the nonelectroactive Lys/L-Cys film provides potential for fabricating novel switchable electrochemical biosensors and bioelectronic devices.

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Stimuli‐Responsive Biointerface Based on Polymer Brushes for Glucose Detection

et al. 2014

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The construction and characterization of a biosensor based on polymer brushes is reported. The use of polymer brushes combined with nanoparticles was applied to show its suitability as a biosensor platform – with glucose oxidase as an enzyme probe. The biosensor demonstrated a pH‐sensitive on‐off property, and it was further used to control or modulate the electrochemical responses. In terms of the kinetic behavior, we were able to show the changing in the kinetic parameters of glucose oxidase operating in “on” and “off” state of the polymer brushes. The performance of the bioelectrode was investigated by chronoamperometry, impedance electrochemistry and cyclic voltammetric techniques. At optimized experimental conditions the dynamic concentration range was 2.0 to 16.0 mmol L−1 with a detection limit of 5.6×10–6 mol L−1. The repeatability of current responses for injections of 5.0 mmol L−1 glucose was evaluated to be 5.3 % (n=10). The resulting biosensor seemed to provide the enzymes with a biocompatible nanoenvironment as it sustained the enhanced enzyme activity for an extended time and promoted possible good electron transfer through the polymer brushes to the electrode.

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pH-tunable bioelectrocatalysis based on layer-by-layer films assembled with TiO2 nanoparticles and poly(allylamine hydrochloride)

Cited by 7 publications

References 52 publications

Switchable Bioelectrocatalysis of Glucose Oxidase Immobilized into Multilayers with Lamellar Nanoparticles of Amino-Functionalized Magnesium Phyllosilicate Clay

Switchable Bioelectrocatalysis of Glucose Oxidase Immobilized into Multilayers with Lamellar Nanoparticles of Amino-Functionalized Magnesium Phyllosilicate Clay

pH-Controllable Bioelectrocatalysis Based on Surface Charge and Conformation Switching of Nonelectroactive Protein

Stimuli‐Responsive Biointerface Based on Polymer Brushes for Glucose Detection

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