pH-sensing nano-crystals of carbonate apatite: Effects on intracellular delivery and release of DNA for efficient expression into mammalian cells

Chowdhury, Ezharul Hoque; Maruyama, Atsushi; Kano, Arihiro; Nagaoka, Masato; Kotaka, Masahiro; Hirose, Shigehisa; Kunou, Megumi; Akaike, Toshihiro

doi:10.1016/j.gene.2006.02.028

Cited by 81 publications

(71 citation statements)

References 17 publications

(18 reference statements)

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“…Elemental analysis and FT-IR of the lyophilized powder of the sedimented particles suggested the structure of typical carbonate apatite, a major component of hard tissues in our body [20,21]. Xray diffraction patterns demonstrated the widening of the peaks as indicative of poor crystallinity unlike those for highly crystalline hydroxyapatite as formed with CaPi precipitation [20,21]. Thus, the chemical reaction for particle formation takes place among Ca 2+ , 3 4 PO  and 3 HCO  , and DNA can electrostatically be associated with the cationic (Ca 2+ -rich)domains of the particles [20,21].…”

Section: Establishment Of Carbonate Apatite Nanoparticles As Smart Dnmentioning

confidence: 87%

“…Addition of relatively low amount of Ca 2+ (3 -5 mM) to such medium (pH 7.5) containing endogenous Pi, followed by incubation at 37˚C, resulted in microscopically visible particles which were apparently absent in the incubated HBS containing the same doses of total Ca 2+ and Pi, implicating that bicarbonate is one of the reactants in generation of those particles. Elemental analysis and FT-IR of the lyophilized powder of the sedimented particles suggested the structure of typical carbonate apatite, a major component of hard tissues in our body [20,21]. Xray diffraction patterns demonstrated the widening of the peaks as indicative of poor crystallinity unlike those for highly crystalline hydroxyapatite as formed with CaPi precipitation [20,21].…”

Section: Establishment Of Carbonate Apatite Nanoparticles As Smart Dnmentioning

confidence: 96%

“…Xray diffraction patterns demonstrated the widening of the peaks as indicative of poor crystallinity unlike those for highly crystalline hydroxyapatite as formed with CaPi precipitation [20,21]. Thus, the chemical reaction for particle formation takes place among Ca 2+ , 3 4 PO  and 3 HCO  , and DNA can electrostatically be associated with the cationic (Ca 2+ -rich)domains of the particles [20,21]. The two crucial factors for determining transfectionpotency, namely, the number and the average size of the particles are dramatically influenced by the concentrations of calcium, phosphate and bicarbonate, the pH of the medium and the incubation period and temperature (Figure 1).…”

Section: Establishment Of Carbonate Apatite Nanoparticles As Smart Dnmentioning

confidence: 99%

See 2 more Smart Citations

pH-responsive magnesium- and carbonate-substituted apatite nano-crystals for efficient and cell-targeted delivery of transgenes

Chowdhury¹

2013

OJGen

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The short half-lives due to the enzymatic degradation in blood, the lack of tissue targetability and the incapability to passively diffuse across the plasma membrane and smoothly traffic across the harsh intracelluar environment are the major shortcomings for nucleic acid-based potential therapeutics, such as recombinant plasmid and antisense oligonucleotides or small interferring RNA (siRNA). Plasmid DNA containing a gene of interest could have immense impact as a promising therapeutic drug for treating genetic as well as acquired human diseases at the molecular level with high level of efficacy and precision. Thus both viral and non-viral synthetic vectors have been developed in the past decades to address the aforementioned challenges of naked DNA. While in the viral particles plasmid DNA is integrated into the viral genome, in most non-viral cases the DNA being anionic in nature is electrostatically associated with a cationic lipid or polymer forming lipoplex or polyplex, respectively, or a cationized inorganic gold, silica or iron oxide particle. Due to the potential immunogenicity and carcinogenicity issues with the viral particles, non-viral vectors have drawn much more attention for the clinical evaluation. However, the main concern of using non-biodegradable particles, specially the inorganic ones, is the adverse effects owing to their long term interactions with body components. We have recently developed biodegradable pH-sensitive inorganic nanoparticles of Mg/CaPi and carbonate apatite for efficient transgene delivery to primary, cancer and embryonic stem cells, by virtue of their high affinity binding with the DNA, ability to contact the cell membrane by ionic or ligand-receptor interactions and fast dissolution kinectis in endosomal acidic pH facilitating release of the DNA from the dissolving particles and also from the endosomes.

show abstract

Section: Establishment Of Carbonate Apatite Nanoparticles As Smart Dnmentioning

confidence: 87%

Section: Establishment Of Carbonate Apatite Nanoparticles As Smart Dnmentioning

confidence: 96%

Section: Establishment Of Carbonate Apatite Nanoparticles As Smart Dnmentioning

confidence: 99%

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pH-responsive magnesium- and carbonate-substituted apatite nano-crystals for efficient and cell-targeted delivery of transgenes

Chowdhury¹

2013

OJGen

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“…The buffering properties of HAp are important for protecting DNA against DNAse degradation during this process. [74] Additionally, it is worth mentioning that it is not difficult to release DNA from HAp. Only a slight increase in temperature or a decrease in pH is necessary to discharge DNA from HAp [75] .…”

Section: Reintroducing Dna Into the Life Cyclementioning

confidence: 99%

Surviving Mass Extinctions through Biomineralized DNA

Turón

Puiggalı́

Bertrán

et al. 2015

Chemistry A European J

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Even in the worst of conditions, such as those which occurred during mass extinction events, life on Earth never totally stopped. Aggressive chemical and physical attacks able to sterilize or poison living organisms occurred repeatedly. Surprisingly, DNA was not degraded, denatured or modified to the point of losing the capability of transferring the genetic information to the next generations. After the events of mass extinction life was able to survive and thrive. DNA was passed on despite being an extremely fragile biomolecule. The potential implications of hydroxyapatite protection of DNA are discussed including how DNA acts as a template for HAp formation, how cell death can trigger biomineralizatoin, and how DNA can be successfully released from HAp when the conditions are favourable for life.

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“…HAp also has been investigated as a material for drug delivery systems due to its chemical similarity to the inorganic phase of bones as well as its ability to adsorb and release several molecules of biological interest [4][5][6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

Effect of Tryptophan Residues on Adsorption of a Cationic Arginine Rich Polypeptide by Hydroxyapatite Nanoparticles and Following Translocation of the Polypeptide Through a Lipid Vesicle Membrane

Ueno

Shimabayashi

Saito

2011

Phosphorus Research Bulletin

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Hydroxyapatite (abbreviated to HAp), in general, adsorbs a polypeptide, resulting in a HAp-peptide complex. In the present paper the Hap was of a nanosized particle and peptide was poly-L-arginine (abbreviated to poly(Arg)) or copoly(L-arginine/ L-tryptophan = 4:1) (abbreviated to poly(Arg,Trp)). Physicochemical properties of these complexes were quite different with each other due mainly to the presence or absence of tryptophan residues on the polymer chain. The adsorption amount of poly(Arg,Trp) on HAp was much larger than that of poly(Arg). Zeta potential of the HAp-poly(Arg,Trp) complex was far lower than that of the HAp-poly(Arg) complex. Considering these facts, it was concluded that hydrophobic interaction between Trp residues of poly(Arg,Trp) induced an adsorption bilayer on HAp, while poly(Arg) simply formed an adsorption monolayer through electrostatic interaction. On the other hand, structural flexibility of a copolymer and its translocation through a lipid membrane were lower and slower in poly(Arg,Trp) than in poly(Arg).

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pH-sensing nano-crystals of carbonate apatite: Effects on intracellular delivery and release of DNA for efficient expression into mammalian cells

Cited by 81 publications

References 17 publications

pH-responsive magnesium- and carbonate-substituted apatite nano-crystals for efficient and cell-targeted delivery of transgenes

pH-responsive magnesium- and carbonate-substituted apatite nano-crystals for efficient and cell-targeted delivery of transgenes

Surviving Mass Extinctions through Biomineralized DNA

Effect of Tryptophan Residues on Adsorption of a Cationic Arginine Rich Polypeptide by Hydroxyapatite Nanoparticles and Following Translocation of the Polypeptide Through a Lipid Vesicle Membrane

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