pH-Responsive doxorubicin-loaded magnetosomes for magnetic resonance-guided focused ultrasound real-time monitoring and ablation of breast cancer

Tang, Na; Zhu, Yi; Lu, Ziwei; Deng, Jiali; Guo, Jiajing; Ding, Xinyi; Wang, Jingyi; Cao, Rong; Chen, An; Huang, Zhongyi; Lu, Hongwei; Wang, Zhongling

doi:10.1039/d3bm00789h

Cited by 2 publications

(1 citation statement)

References 37 publications

(41 reference statements)

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“…Thanks to the developed methods for cultivating bacteria and extracting nanoparticles from them, MTs were rigorously tested for various applications. These include serving as carriers for anticancer drugs [ 11 , 12 ] and contrast agents in magnetic resonance imaging (MRI) [ 13 , 14 ]. In the context of the reported drug delivery systems, the use of larger carriers to transport MTs may expand their biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Hydrogel-Based Liquid Marbles with Magnetosomes

Bielas,

Kubiak,

Molcan

et al. 2023

Materials

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Liquid marbles are widely known for their potential biomedical applications, especially due to their versatility and ease of preparation. In the present work, we prepared liquid marbles with various cores composed of water, agar-based hydrogels, magnetic fluids, or non-aqueous substances. As a coating material, we used biocompatible particles of plant origin, such as turmeric grains and Lycopodium pollen. Additionally, we provided marbles with magnetic properties by incorporating either magnetosomes or iron oxide nanoparticles as a powder or by injecting another magnetic fluid. Structures obtained in this way were stable and susceptible to manipulation by an external magnetic field. The properties of the magnetic components of our marbles were verified using electron paramagnetic resonance (EPR) spectroscopy and vibrating sample magnetometry (VSM). Our approach to encapsulation of active substances such as antibiotics within a protective hydrogel core opens up new perspectives for the delivery of hydrophobic payloads to the inherently hydrophilic biological environment. Additionally, hydrogel marbles enriched with magnetic materials showed promise as biocompatible heating agents under alternating magnetic fields. A significant innovation of our research was also the fabrication of composite structures in which the gel-like core was surrounded without mixing by a magnetic fluid covered on the outside by the particle shell. Our liquid marbles, especially those with a hydrogel core and magnetic content, due to the ease of preparation and favorable properties, have great potential for biomedical use. The fact that we were able to simultaneously produce, functionalize (by filling with predefined cargo), and manipulate (by means of an external magnetic field) several marbles also seems to be important from an application point of view.

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Section: Introductionmentioning

confidence: 99%

Biocompatible Hydrogel-Based Liquid Marbles with Magnetosomes

Bielas,

Kubiak,

Molcan

et al. 2023

Materials

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Development of Focused Ultrasound-Assisted Nanoplexes for RNA Delivery

Ranjan,

Bosch,

Lukkari

et al. 2024

Nanomaterials

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RNA-based therapeutics, including siRNA, have obtained recognition in recent years due to their potential to treat various chronic and rare diseases. However, there are still limitations to lipid-based drug delivery systems in the clinical use of RNA therapeutics due to the need for optimization in the design and the preparation process. In this study, we propose adaptive focused ultrasound (AFU) as a drug loading technique to protect RNA from degradation by encapsulating small RNA in nanoliposomes, which we term nanoplexes. The AFU method is non-invasive and isothermal, as nanoplexes are produced without direct contact with any external materials while maintaining precise temperature control according to the desired settings. The controllability of sample treatments can be effectively modulated, allowing for a wide range of ultrasound intensities to be applied. Importantly, the absence of co-solvents in the process eliminates the need for additional substances, thereby minimizing the potential for cross-contaminations. Since AFU is a non-invasive method, the entire process can be conducted under sterile conditions. A minimal volume (300 μL) is required for this process, and the treatment is speedy (10 min in this study). Our in vitro experiments with silencer CD44 siRNA, which performs as a model therapeutic drug in different mammalian cell lines, showed encouraging results (knockdown > 80%). To quantify gene silencing efficacy, we employed quantitative polymerase chain reaction (qPCR). Additionally, cryo-electron microscopy (cryo-EM) and atomic force microscopy (AFM) techniques were employed to capture images of nanoplexes. These images revealed the presence of individual nanoparticles measuring approximately 100–200 nm in contrast with the random distribution of clustered complexes observed in ultrasound-untreated samples of liposome nanoparticles and siRNA. AFU holds great potential as a standardized liposome processing and loading method because its process is fast, sterile, and does not require additional solvents.

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pH-Responsive doxorubicin-loaded magnetosomes for magnetic resonance-guided focused ultrasound real-time monitoring and ablation of breast cancer

Abstract: We have successfully prepared pH-responsive doxorubicin-loaded magnetosomes as an integrated diagnostic and theranostic nanoplatform that is responsive to an acidic microenvironment and can be a synergistic sensitizer for MRgFUS ablation therapy.

Cited by 2 publications

References 37 publications

Biocompatible Hydrogel-Based Liquid Marbles with Magnetosomes

Biocompatible Hydrogel-Based Liquid Marbles with Magnetosomes

Development of Focused Ultrasound-Assisted Nanoplexes for RNA Delivery

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