pH-responsive allochroic nanoparticles for the multicolor detection of breast cancer biomarkers

Xu, Weiqing; Jiao, Lei; Ye, Huarong; Guo, Zhenzhong; Wu, Yu; Yan, Hongye; Gu, Wenling; Du, Dan; Lin, Yuehe; Zhu, Chengzhou

doi:10.1016/j.bios.2019.111780

Cited by 41 publications

(25 citation statements)

References 41 publications

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“…Given these facts, we deduce that the nanotags can be used to realize the colorimetric detection of heterogeneous CTCs. Based on the standard curves, the loading amounts of TP and CUR on MoS 2 are about 79.8 and 82.1%, respectively (Figure S6), which are comparable to these in literatures. , …”

Section: Resultssupporting

confidence: 85%

“…Based on the standard curves, the loading amounts of TP and CUR on MoS 2 are about 79.8 and 82.1%, respectively (Figure S6), which are comparable to these in literatures. 35,36 Optimization of Experimental Conditions. Before evaluating the performance of colorimetric detection, a series of experimental parameters need to be optimized.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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pH-Sensitive Dye-Based Nanobioplatform for Colorimetric Detection of Heterogeneous Circulating Tumor Cells

et al. 2021

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The efficient capture and sensitive detection of circulating tumor cells (CTCs) play a vital role in cancer diagnosis and prognosis. However, CTCs in the peripheral blood are very rare and heterogeneous, which make them difficult to isolate and detect. Herein, a novel colorimetric nanobioplatform was successfully developed for the highly efficient capture and highly sensitive detection of heterogeneous CTCs, which consisted of two parts: the multivalent aptamer-modified gold nanoparticles as the capture unit and two kinds of aptamer-functionalized pH-sensitive allochroic dyes (thymolphthalein and curcumin) @ molybdenum disulfide nanoflakes (MoS 2 NFs) acting as the visual simultaneous detection of heterogeneous CTCs. Using MCF-7 and HeLa cells as the CTC models, the capture unit can effectively isolate the CTCs due to the multivalent probe with improved affinity. The two allochroic dyes can display obvious color changes under alkaline conditions (pH 12.5) in the presence of MCF-7 and HeLa cells, which provided a rapid and sensitive strategy for visualizing simultaneous detection of heterogeneous CTCs as low as 5 cells mL −1 . This nanoplatform possessed a high sensitivity toward CTC detection owing to high dye loading capacity of MoS 2 NFs and allochroic dyes with excellent pH sensitivity. It can successfully distinguish and quantitatively detect the targeted heterogeneous CTCs from numerous interfering cells in diluted whole blood. It can also be used to detect CTCs from lysed blood samples from cancer patients, indicating promising application for cancer diagnosis.

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Section: Resultssupporting

confidence: 85%

Section: ■ Results and Discussionmentioning

confidence: 99%

pH-Sensitive Dye-Based Nanobioplatform for Colorimetric Detection of Heterogeneous Circulating Tumor Cells

et al. 2021

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“…The more sensitive pH sensing schemes can be explored to improve the analytical parameters of the developed method. Recently, Xu et al prepared pH-responsive allochroic nanoparticles and used for the colorimetric detection of breast cancer biomarkers [29]. A fluorescence-based biosensor was developed for the detection Biotechnology and Applied Biochemistry…”

Section: Effect Of Lighting Conditions On Stability Of Color Parametementioning

confidence: 99%

Enzyme‐based detection of epoxides using colorimetric assay integrated with smartphone imaging

Gul

Bogale

Deng

et al. 2020

Biotech and App Biochem

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Epoxides are widely used chemicals, the determination of which is of paramount importance. Herein, we present an enzyme‐based approach for noninstrumental detection of epoxides in standard solution and environmental samples. Halohydrin dehalogenase (HheC) as a biological recognition element and epichlorohydrin as a model analyte were evaluated for sensing. The detection is based on the color change of the pH indicator dye bromothymol blue caused by the HheC‐catalyzed ring‐opening of the epoxide substrate. The color change is then exploited for the determination of epoxide using a smartphone as an image acquisition and data processing device, eliminating the need for computer‐based image analysis software. The color parameters were systematically evaluated to determine the optimum quantitative analytical parameter. Under optimal conditions, the proposed enzyme‐based detection system showed a linear range of 0.13–2 mM with a detection limit of 0.07 mM and an assay time of 8 Min. In addition, the repeatability expressed as relative standard deviation was found to be below 5% (n = 6). Validation with gas chromatographic analyses showed that the proposed enzyme‐based epoxide detection could be an alternative way in the quantitative determination of epoxides, and particularly useful in resource‐limited settings.

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“…As a typical bioanalytical and immunological tool, enzyme-linked immunosorbent assay (ELISA) is widely regarded as the “gold standard” and performed for the detection of various biomarkers in clinical diagnosis. − The key point of ELISA is the catalytic activity and stability of horseradish peroxidase (HRP) . However, hindered by intrinsic fragility, the unique spatial geometry of HRP can be destroyed in extreme analytical conditions, resulting in variability and false-positive results .…”

Section: Introductionmentioning

confidence: 99%

Axial Ligand-Engineered Single-Atom Catalysts with Boosted Enzyme-Like Activity for Sensitive Immunoassay

Song

Kang

et al. 2021

Anal. Chem.

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Inspired by the key role of the coordination environment in the catalytic activity of enzymes, a rational design of the coordination structure of active sites at the atom scale is expected to develop high-performance enzyme-like catalysts. Here, we design a simple model system involving pentacoordinated and tetracoordinated Fe–N–C single-atom catalysts (named NG-Heme and G-Heme, respectively) to investigate structure–activity relationships. NG-Heme with axial ligand-engineered Fe sites exhibits superior enzyme-like activity to G-Heme, achieving the goal of vivid mimicking of the active sites of peroxidase. Experiments and theoretical studies reveal that the enhanced intrinsic catalytic activity originates from the “push effect” of the additional axial ligand, which can strengthen the interaction between the active site and the intermediate. Based on the outstanding catalytic activity, an NG-Heme-linked immunosorbent assay was constructed for colorimetric detection of carcinoembryonic antigen, exhibiting satisfactory sensitivity and feasibility in the analysis of clinical samples.

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pH-responsive allochroic nanoparticles for the multicolor detection of breast cancer biomarkers

Cited by 41 publications

References 41 publications

pH-Sensitive Dye-Based Nanobioplatform for Colorimetric Detection of Heterogeneous Circulating Tumor Cells

pH-Sensitive Dye-Based Nanobioplatform for Colorimetric Detection of Heterogeneous Circulating Tumor Cells

Enzyme‐based detection of epoxides using colorimetric assay integrated with smartphone imaging

Axial Ligand-Engineered Single-Atom Catalysts with Boosted Enzyme-Like Activity for Sensitive Immunoassay

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