2014
DOI: 10.1002/elps.201300484
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pH‐regulated formation of side products in the reductive amination approach for differential labeling of peptides in relative quantitative experiments

Abstract: Research Article pH-regulated formation of side products in the reductive amination approach for differential labeling of peptides in relative quantitative experimentsAmong the most common stable-isotope labeling strategies, the reaction of formaldehyde with peptides in the presence of NaCNBH 3 features many attractive aspects that are conducive to its employment in quantitation experiments in proteomics. Reductive amination, with formaldehyde and d(2)-formaldehyde, is reported to be a fast, easy, and specific… Show more

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Cited by 2 publications
(2 citation statements)
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“…Deuterium labeling was performed by similar procedure but by using formaldehyde- D 2 (20% in water). An extensive characterization of dimethylation in quantitative analysis has been recently performed by our group, demonstrating, in accordance with other published works, , that this approach is cheap, quick, and applicable to any sample, including complex samples. The completion of the reaction was evaluated by running singularly the D 0 - and D 2 -labeled samples in nL–MS/MS by using a Q-Tof Premier mass spectrometer (Waters, Manchester, U.K.) operating in DDA mode .…”
Section: Methodssupporting
confidence: 84%
“…Deuterium labeling was performed by similar procedure but by using formaldehyde- D 2 (20% in water). An extensive characterization of dimethylation in quantitative analysis has been recently performed by our group, demonstrating, in accordance with other published works, , that this approach is cheap, quick, and applicable to any sample, including complex samples. The completion of the reaction was evaluated by running singularly the D 0 - and D 2 -labeled samples in nL–MS/MS by using a Q-Tof Premier mass spectrometer (Waters, Manchester, U.K.) operating in DDA mode .…”
Section: Methodssupporting
confidence: 84%
“…Here we report a novel 3-plexed diethylation (DE) method for highly accurate quantitative proteomics, in which DE is acetaldehyde-based reductive alkylation of peptides. Because all mass shifts are introduced by 13 C isotopologues of acetaldehyde, our DE-3plex method is free from the deuterium effect; therefore, the multiplexed peptides are coeluted in LC–MS analysis. Also, 13 C isotopologues of acetaldehyde are all commercially available and cost-effective overall (<$10 for labeling of 100 μg proteome).…”
Section: Introductionmentioning
confidence: 99%