pH-Degradable Mannosylated Nanogels for Dendritic Cell Targeting

Coen, Ruben De; Vanparijs, Nane; Risseeuw, Martijn; Lybaert, Lien; Louage, Benoit; Koker, Stefaan De; Kumar, V. S. Senthil; Grooten, Johan; Taylor, Leeanne; Ayres, Neil; Calenbergh, Serge Van; Nuhn, Lutz; Geest, Bruno G. De

doi:10.1021/acs.biomac.6b00685

Cited by 66 publications

(51 citation statements)

References 62 publications

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“…In a separate study, the De Geest group used a templating technique to prepare polymer nanogels to promote CTL priming in vivo . Also in the same year, the same group developed mannosylated nanogels made from tetra‐ O ‐acetyl‐α‐ d ‐mannosylethyl acrylamide and poly(pentafluorophenyl acrylate), which is pH‐degradable for endosomal release of antigens …”

Section: Nanoscale Materials For Immunotherapymentioning

confidence: 99%

Materials for Immunotherapy

et al. 2019

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Breakthroughs in materials engineering have accelerated the progress of immunotherapy in preclinical studies. The interplay of chemistry and materials has resulted in improved loading, targeting, and release of immunomodulatory agents. An overview of the materials that are used to enable or improve the success of immunotherapies in preclinical studies is presented, from immunosuppressive to proinflammatory strategies, with particular emphasis on technologies poised for clinical translation. The materials are organized based on their characteristic length scale, whereby the enabling feature of each technology is organized by the structure of that material. For example, the mechanisms by which i) nanoscale materials can improve targeting and infiltration of immunomodulatory payloads into tissues and cells, ii) microscale materials can facilitate cell‐mediated transport and serve as artificial antigen‐presenting cells, and iii) macroscale materials can form the basis of artificial microenvironments to promote cell infiltration and reprogramming are discussed. As a step toward establishing a set of design rules for future immunotherapies, materials that intrinsically activate or suppress the immune system are reviewed. Finally, a brief outlook on the trajectory of these systems and how they may be improved to address unsolved challenges in cancer, infectious diseases, and autoimmunity is presented.

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Section: Nanoscale Materials For Immunotherapymentioning

confidence: 99%

Materials for Immunotherapy

et al. 2019

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“…Furthermore, remaining PFP esters could be converted into hydrophilic moieties to yield fully hydrated nanogels after transfer to aqueous medium. In addition, we also recently demonstrated the use of this approach to engineer nanogels with dendritic cell-targeting properties by replacing the mTEGMA block by a mannosylated glycopolymer block [24].…”

mentioning

confidence: 99%

Core/shell Protein-Reactive Nanogels Via a Combination of RAFT Polymerization and Vinyl Sulfone Postmodification

Vanparijs

Nuhn

Paluck

et al. 2016

Nanomedicine (Lond.)

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Aim: A promising nanogel vaccine platform was expanded toward antigen conjugation. Materials & methods: Block copolymers containing a reactive ester solvophobic block and a PEG-like solvophilic block were synthesized via reversible additionfragmentation chain-transfer polymerization. Following self-assembly in DMSO, the esters allow for core-crosslinking and hydrophilization by amide bond formation with primary amines. Free thiols were accessed at the polymer chain ends through aminolysis of the reversible addition-fragmentation chain-transfer groups, and into the nanogel core by reactive ester conversion with cysteamine. Subsequently, free thiols were converted into vinyl sulfone moieties. Results: Despite sterical constraints, nanogel-associated vinyl sulfone moieties remained well accessible for cysteins to enforce protein conjugation successfully. Conclusion: Our present findings provide a next step toward well-defined vaccine nanoparticles that can co-deliver antigen and a molecular adjuvant.

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“…Glycosylated nanogels were prepared via core crosslinking of amphiphilic copolymers composed of an acetylated glycosylated block and a PFP activated ester block. A one‐pot approach of self‐assembly, pH‐sensitive crosslinking, and removal of remaining PFP esters and protecting groups yielded in fully hydrated nanogels . In a follow up study, De Geest and co‐workers furthermore introduced cationic groups to the core forming block after crosslinking with an pH‐responsive ketal crosslinker via PFP ester chemistry .…”

Section: Active Esters In Particle Analogous Reactionsmentioning

confidence: 99%

Reactive Precursor Particles as Synthetic Platform for the Generation of Functional Nanoparticles, Nanogels, and Microgels

Gruber

Navarro

Klinger

2019

Adv Materials Inter

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Precise control of the chemical functionality of polymer nanoparticles is a key requirement in tailoring their (dynamic) colloidal properties toward advanced applications. However, current synthetic techniques are still limited in the versatility of chemical design and preparation of such functional colloidal nanomaterials. Two major challenges remain: First, various particle preparation methods are restricted in their functional group tolerance, thus hindering certain combinations of polymer backbones with specific functional groups. Second, the preparation of particles with different functionalities requires the synthesis of different particle batches. But this often results in a simultaneous variation of colloidal features. As a result, the accurate determination of important structure–property relations is still hindered. To address these restrictions, postmodification of preformed reactive particles is gaining more attention. This technique has evolved from polymer synthesis, where postpolymerization functionalization enables the introduction of a plethora of functional groups without changing the degree of polymerization and the molecular weight distribution. Similarly, modifying precursor particles enables the introduction of functional groups into particles while reducing variations in colloidal features, e.g., particle size and size distribution. This powerful synthetic method complements established procedures for functionalization of particle surfaces, thereby enabling the facile preparation of (multi‐)functional particle libraries, which will allow precise investigations of structure–property relations.

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pH-Degradable Mannosylated Nanogels for Dendritic Cell Targeting

Cited by 66 publications

References 62 publications

Materials for Immunotherapy

Materials for Immunotherapy

Core/shell Protein-Reactive Nanogels Via a Combination of RAFT Polymerization and Vinyl Sulfone Postmodification

Reactive Precursor Particles as Synthetic Platform for the Generation of Functional Nanoparticles, Nanogels, and Microgels

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