pH-controllable cell-penetrating polypeptide that exhibits cancer targeting

Lee, DaeYong; Noh, Ilkoo; Yoo, Jisang; Rejinold, N. Sanoj; Kim, Yeu‐Chun

doi:10.1016/j.actbio.2017.05.040

Cited by 23 publications

(24 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In ACPPs, tumor microenvironment responsive materials are exploited to display CPPs either at lower pH or in presence of overexpression of ECM development-remodeling proteases, or in response to external stimuli of heat or light to a disease site (MacEwan and Chilkoti, 2013 ). Lee et al ( 2017 ) synthesized poly-l-lysine-based pH-controllable CPPs which undergo pH-dependent conformational transition and display CPPs at the target site. It was reported that at physiological pH, the electrostatic attractions existing between carboxylate and protonated amine groups in side chain of CPPs contributed to its low helical tendency, thereby preventing unselective cellular internalization.…”

Section: Types Of Nanocarriersmentioning

confidence: 99%

“…It was reported that at physiological pH, the electrostatic attractions existing between carboxylate and protonated amine groups in side chain of CPPs contributed to its low helical tendency, thereby preventing unselective cellular internalization. However, in tumor acidic microenvironment, the helical conformation was attained enabling cell-penetrating properties at specific cancer sites (Lee et al, 2017 ). Zhang Y. et al ( 2017 ) developed DOX- and vincristine-loaded liposomes, which were modified with 2 ligands, i.e., T7, seven-peptide ligand of transferrin receptors (TfR) and D A7R, d-peptide ligand of VEGFR 2 for efficient targeting to glioma.…”

Section: Types Of Nanocarriersmentioning

confidence: 99%

See 1 more Smart Citation

Tumor Microenvironment Targeted Nanotherapy

2018

View full text Add to dashboard Cite

Recent developments in nanotechnology have brought new approaches to cancer diagnosis and therapy. While enhanced permeability and retention effect promotes nano-chemotherapeutics extravasation, the abnormal tumor vasculature, high interstitial pressure and dense stroma structure limit homogeneous intratumoral distribution of nano-chemotherapeutics and compromise their imaging and therapeutic effect. Moreover, heterogeneous distribution of nano-chemotherapeutics in non-tumor-stroma cells damages the non-tumor cells, and interferes with tumor-stroma crosstalk. This can lead not only to inhibition of tumor progression, but can also paradoxically induce acquired resistance and facilitate tumor cell proliferation and metastasis. Overall, the tumor microenvironment plays a vital role in regulating nano-chemotherapeutics distribution and their biological effects. In this review, the barriers in tumor microenvironment, its consequential effects on nano-chemotherapeutics, considerations to improve nano-chemotherapeutics delivery and combinatory strategies to overcome acquired resistance induced by tumor microenvironment have been summarized. The various strategies viz., nanotechnology based approach as well as ligand-mediated, redox-responsive, and enzyme-mediated based combinatorial nanoapproaches have been discussed in this review.

show abstract

Section: Types Of Nanocarriersmentioning

confidence: 99%

Section: Types Of Nanocarriersmentioning

confidence: 99%

Tumor Microenvironment Targeted Nanotherapy

2018

View full text Add to dashboard Cite

show abstract

“…Both cell types were treated with increasing concentrations of peptide for 2 and 24 h. ACPs are known to first interact with negatively charged membranes (i.e., cancer cell membranes) via electrostatic interactions, after which they tend to adopt helical conformations, which causes cell membrane permeabilization or even membrane disruption that may lead to necrosis [ 33 ]. These peptides may also be internalized into the cell, leading to the disruption of the mitochondrial membrane and causing apoptosis [ 33 ]. Torres et al [ 9 ] described similar helical structure propensity and physicochemical properties for Dec-NH 2 and [Leu] 10 -Dec-NH 2 .…”

Section: Resultsmentioning

confidence: 99%

Natural and redesigned wasp venom peptides with selective antitumoral activity

Torres¹,

Andrade²,

Sato³

et al. 2018

Beilstein J. Org. Chem.

View full text Add to dashboard Cite

About 1 in 8 U.S. women (≈12%) will develop invasive breast cancer over the course of their lifetime. Surgery, chemotherapy, radiotherapy, and hormone manipulation constitute the major treatment options for breast cancer. Here, we show that both a natural antimicrobial peptide (AMP) derived from wasp venom (decoralin, Dec-NH2), and its synthetic variants generated via peptide design, display potent activity against cancer cells. We tested the derivatives at increasing doses and observed anticancer activity at concentrations as low as 12.5 μmol L−1 for the selective targeting of MCF-7 breast cancer cells. Flow cytometry assays further revealed that treatment with wild-type (WT) peptide Dec-NH2 led to necrosis of MCF-7 cells. Additional atomic force microscopy (AFM) measurements indicated that the roughness of cancer cell membranes increased significantly when treated with lead peptides compared to controls. Biophysical features such as helicity, hydrophobicity, and net positive charge were identified to play an important role in the anticancer activity of the peptides. Indeed, abrupt changes in peptide hydrophobicity and conformational propensity led to peptide inactivation, whereas increasing the net positive charge of peptides enhanced their activity. We present peptide templates with selective activity towards breast cancer cells that leave normal cells unaffected. These templates represent excellent scaffolds for the design of selective anticancer peptide therapeutics.

show abstract

“…The TH analogues formed were conjugated to a camptothecin (Cpt)-and butyl-TH-modified conjugate and displayed a pronounced cytotoxicity effect on cancer cells more strongly in a pH-dependent manner compared to TH and other conjugates. 82 Very recently, new pH-controllable CPPs (PCCPPs) were reported on by Lee et al 83 According to this study, synthesized poly-l-lysine-based PCCPPs were capable of undergoing pH-dependent conformational transition, thereby displaying specific cell-penetrating properties at the target site. At a physiological pH, the PCCPPs contained a low helical tendency due to electrostatic attractions between carboxylate and protonated amine groups in each side chain.…”

Section: Acid-activated Cppsmentioning

confidence: 99%

Tumor-targeting delivery of herb-based drugs with cell-penetrating/tumor-targeting peptide-modified nanocarriers

Kebebe¹,

Liu²,

Wu³

et al. 2018

IJN

View full text Add to dashboard Cite

Cancer has become one of the leading causes of mortality globally. The major challenges of conventional cancer therapy are the failure of most chemotherapeutic agents to accumulate selectively in tumor cells and their severe systemic side effects. In the past three decades, a number of drug delivery approaches have been discovered to overwhelm the obstacles. Among these, nanocarriers have gained much attention for their excellent and efficient drug delivery systems to improve specific tissue/organ/cell targeting. In order to enhance targeting efficiency further and reduce limitations of nanocarriers, nanoparticle surfaces are functionalized with different ligands. Several kinds of ligand-modified nanomedicines have been reported. Cell-penetrating peptides (CPPs) are promising ligands, attracting the attention of researchers due to their efficiency to transport bioactive molecules intracellularly. However, their lack of specificity and in vivo degradation led to the development of newer types of CPP. Currently, activable CPP and tumor-targeting peptide (TTP)-modified nanocarriers have shown dramatically superior cellular specific uptake, cytotoxicity, and tumor growth inhibition. In this review, we discuss recent advances in tumor-targeting strategies using CPPs and their limitations in tumor delivery systems. Special emphasis is given to activable CPPs and TTPs. Finally, we address the application of CPPs and/or TTPs in the delivery of plant-derived chemotherapeutic agents.

show abstract

pH-controllable cell-penetrating polypeptide that exhibits cancer targeting

Cited by 23 publications

References 35 publications

Tumor Microenvironment Targeted Nanotherapy

Tumor Microenvironment Targeted Nanotherapy

Natural and redesigned wasp venom peptides with selective antitumoral activity

Tumor-targeting delivery of herb-based drugs with cell-penetrating/tumor-targeting peptide-modified nanocarriers

Contact Info

Product

Resources

About