PGS:Gelatin nanofibrous scaffolds with tunable mechanical and structural properties for engineering cardiac tissues

Kharaziha, Mahshid; Nikkhah, Mehdi; Shin, Su Ryon; Annabi, Nasim; Masoumi, Nafiseh; Gaharwar, Akhilesh K.; Camci‐Unal, Gulden; Khademhosseini, Ali

doi:10.1016/j.biomaterials.2013.04.045

Cited by 279 publications

(212 citation statements)

References 62 publications

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“…Another important factor to consider is the scaffold chemistry which has a profound effect on cellular response (Saha et al 2007;Kharaziha et al 2013;Shah et al 2014;Collins & Birkinshaw 2013). …”

Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…Whereas, in another study, PGS and gelatin were blended before electrospinning resulting to the fabrication of scaffolds with well-defined anisotropy that can mimic the left ventricular myocardium architecture [110]. From the in vitro studies using neonatal rat cardiac fibroblast cells (CFs) and CMs, it was found that aligned fibrous scaffold, consisting of 33 wt.…”

Section: Poly(glycerol Sebacate)-based Scaffoldsmentioning

confidence: 99%

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

et al. 2017

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Please cite this article as: Kitsara, M., Agbulut, O., Kontziampasis, D., Chen, Y., Menasché, P., Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering, Acta Biomaterialia (2016), doi: http://dx.doi.org/ 10. 1016/j.actbio.2016.11.014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractCardiac cell therapy holds a real promise for improving heart function and especially of the chronically failing myocardium. Embedding cells into 3D biodegradable scaffolds may better preserve cell survival and enhance cell engraftment after transplantation, consequently improving cardiac cell therapy compared with direct intramyocardial injection of isolated cells.The primary objective of a scaffold used in tissue engineering is the recreation of the natural 3D environment most suitable for an adequate tissue growth. An important aspect of this commitment is to mimic the fibrillar structure of the extracellular matrix, which provides essential guidance for cell organization, survival, and function. Recent advances in nanotechnology have significantly improved our capacities to mimic the extracellular matrix. Among them, electrospinning is well known for being easy to process and cost effective. Consequently, it is becoming increasingly popular for biomedical applications and it is most definitely the cutting edge technique to make scaffolds that mimic the extracellular matrix for industrial applications.Here, the desirable physico-chemical properties of the electrospun scaffolds for cardiac therapy are described, and polymers are categorized to natural and synthetic. Moreover, the methods used for improving functionalities by providing cells with the necessary chemical cues and a more in vivo-like environment are reported.

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“…In this approach, cells are seeded onto the scaffolds before going through tissue maturation. In scaffold fabrication, several methods, such as solvent casting [28][29][30] , molding [31] and electrospinning [32][33][34][35][36][37][38] , have been used. Features on the fabricated scaffolds can affect cell responses directly.…”

Section: Conventional Techniques In Cardiac Tissue Engineeringmentioning

confidence: 99%

Bioprinting in cardiovascular tissue engineering: a review

Lee¹,

Sing²,

Tan³

et al. 2016

ijb

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Fabrication techniques for cardiac tissue engineering have been evolving around scaffold-based and scaffold-free approaches. Conventional fabrication approaches lack control over scalability and homogeneous cell distribution. Bioprinting provides a technological platform for controlled deposition of biomaterials, cells, and biological factors in an organized fashion. Bioprinting is capable of alternating heterogeneous cell printing, printing anatomical relevant structure and microchannels resembling vasculature network. These are essential features of an engineered cardiac tissue. Bioprinting can potentially build engineered cardiac construct that resembles native tissue across macro to nanoscale.

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PGS:Gelatin nanofibrous scaffolds with tunable mechanical and structural properties for engineering cardiac tissues

Cited by 279 publications

References 62 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

Bioprinting in cardiovascular tissue engineering: a review

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