PGPR-induced defense responses in the soybean plant against charcoal rot disease

Mufti, Rabia; Bano, Asghari

doi:10.1007/s10658-019-01828-6

Cited by 13 publications

(9 citation statements)

References 54 publications

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“…In vitro and greenhouse testing of our newly isolated strains, P. putida and B. clausii produced prominent results regarding the root length of plants in the case of nonpathogenic conditions as well as in case of fungal stress on plants thereby increasing plant survival rates. Similar results were reported in many studies by the application of PGPB in stressed and non-stressed conditions in soybean and other plants [6,49].…”

Section: Discussionsupporting

confidence: 91%

“…Similar results are reported in various studies concerning the positive effects of PGPB on stressed and unstressed plants [23,50,51]. The positive results for root and shoot weights are correlated with the previous studies involving the exploitation of PGPB to reduce disease severity and at the same time strengthen their growth parameters [6,23,51]. Adequate leaf area for plants facilitates the absorption of sunlight and results in the promotion of photosynthetic processes in plants to produce an enhanced amount of photosynthetic products which increase the degree of well-being for plants.…”

Section: Discussionsupporting

confidence: 89%

“…Our findings proved that PGPB can induce activated antioxidant activities in stressed plants to reduce damage caused by diseases. Similar effects of PGPB on catalase in diseased plants are reported [6]. The role of POD and APX is mainly the degradation of hydrogen peroxide radicals; their activities were boosted in stressed plants as compared to control substantially by P. putida as compared to B. clausii whose effect was less pronounced; these results are same as indicated by different studies on various plant stresses involving the use of different PGPB [6,12,23,51].…”

Section: Discussionsupporting

confidence: 79%

“…Remnants of infected plants in the soil incubate the pathogen M. phaseolina which subsequently infects the seeds and roots of next crop. It penetrates the surface of roots through germination tubes formed from sclerotia [6]. In the early stages of infection, from 7-42 days, mycelia of fungus perforate in the epidermis of soybean.…”

Section: Introductionmentioning

confidence: 99%

“…Elevated soil temperatures and low moisture content of soil increase the growth of M. phaseolina [7]. Early morphological indication of charcoal rot is rolling and wilting of leaves along with their reduced size [6].…”

Section: Introductionmentioning

confidence: 99%

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Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina phaseolina in Soybean (Glycine max L.)

et al. 2020

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Controlling agricultural pests using suitable biocontrol agents has been considered the best strategy for sustainable agriculture. Charcoal rot caused by a necrotrophic fungus Macrophomina phaseolina is responsible for a 30–50% annual reduction in soybean yield worldwide. Little is known about the role of Bacillus clausii in reducing charcoal rot disease severity in the soybean crop. In this study, we investigated plant growth promoting and antagonistic potential of Pseudomonas putida (MT604992) and Bacillus clausii (MT604989) against charcoal rot disease incidence in soybean. Among twenty bacteria isolated from soil and water samples of two different hot springs of Gilgit-Baltistan, Pakistan, 80% were siderophore positive; 65% were hydrogen cyanide (HCN) positive; 55%, 30%, and 75% were phosphate, potassium, and zinc solubilizers, respectively. Based on higher antagonistic activities and plant growth promoting traits five strains were selected for in vitro screening. Out of all tested strains, Pseudomonas putida and Bacillus clausii showed a significant increase in germination, growth, and disease suppression in soybean. These strains produced a pronounced increase in relative water content, photosynthetic pigments, membrane stability, proline, antioxidant enzymes status, phytohormones content (Salicylic acid, and Jasmonic acid), and disease suppression in comparison to control plants. Bacillus clausii mitigated the disease by 97% with a marked increase in the proline content (73% and 89%), superoxide dismutase (356% and 208%), peroxidase (439% and 138.6%), catalase (255.8% and 80.8%), and ascorbate peroxidase (228% and 90%) activities in shoots and roots, respectively. Infected plants showed an increase in salicylic acid and jasmonic acid content which was further increased with the application of the selected strains to increase resistance against pathogens. To our knowledge, this is the first study showing a rise in salicylic acid and jasmonic acid in Macrophomina phaseolina infected plants. These two strains are suggested as a cost-effective, eco-friendly, and sustainable alternative to chemical fungicides. However, there is a need to explore the field testing and molecular mechanisms leading to disease suppression by these strains.

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Section: Discussionsupporting

confidence: 91%