PGP9.5, a ubiquitin C-terminal hydrolase; pattern of mRNA and protein expression during neural development in the mouse

Schofield, Julian; Day, Ian N.M.; Thompson, R.J.; Edwards, Yvonne H.

doi:10.1016/0165-3806(94)00217-n

Cited by 87 publications

(57 citation statements)

References 28 publications

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“…Traditionally, these endings are detected by anti-PGP9.5 antibody staining (Fundin et al, 1997a;Navarro et al, 1997;Rice et al, 1997). Note that anti-PGP9.5 labels all neurons, including sympathetic and motor neurons as well as sensory neurons (Schofield et al, 1995). In addition, we found that anti-PGP9.5 antibody also stains blood cells, but the staining appears to be nonspecific.…”

Section: Generation Of Avil-hplap Micementioning

confidence: 59%

Analyzing Somatosensory Axon Projections with the Sensory Neuron-SpecificAdvillinGene

Hasegawa¹,

Abbott²,

Han³

et al. 2007

J. Neurosci.

151

173

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Peripheral sensory neurons detect diverse physical stimuli and transmit the information into the CNS. At present, the genetic tools for specifically studying the development, plasticity, and regeneration of the sensory axon projections are limited. We found that the gene encoding Advillin, an actin binding protein that belongs to the gelsolin superfamily, is expressed almost exclusively in peripheral sensory neurons. We next generated a line of knock-in mice in which the start codon of the Advillin is replaced by the gene encoding human placenta alkaline phosphatase (Avil-hPLAP mice). In heterozygous Avil-hPLAP mice, sensory axons, the exquisite sensory endings, as well as the fine central axonal collaterals can be clearly visualized with a simple alkaline phosphatase staining. Using this mouse line, we found that the development of peripheral target innervation and sensory ending formation is an ordered process with specific timing depending on sensory modalities. This is also true for the in-growth of central axonal collaterals into the brainstem and the spinal cord. Our results demonstrate that Avil-hPLAP mouse is a valuable tool for specifically studying peripheral sensory neurons. Functionally, we found that the regenerative axon growth of Advillin-null sensory neurons is significantly shortened and that deletion of Advillin reduces the plasticity of whisker-related barrelettes patterns in the hindbrain.

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Section: Generation Of Avil-hplap Micementioning

confidence: 59%

Analyzing Somatosensory Axon Projections with the Sensory Neuron-SpecificAdvillinGene

Hasegawa¹,

Abbott²,

Han³

et al. 2007

J. Neurosci.

151

173

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“…7 PGP9.5 is a ubiquitin hydrolase widely expressed in neuronal tissues at all stages of neuronal differentiation. 8,9 Ubiquitination of cellular proteins and targeting them for subsequent degradation via ubiquitin-mediated proteolysis is potentially an important mechanism that regulates cell cycle genes. 10,11 In tumors, increased deubiquitination of cyclins by PGP9.5 could contribute to the uncontrolled growth of somatic cells.…”

mentioning

confidence: 99%

PGP9.5 As a Candidate Tumor Marker for Non-Small-Cell Lung Cancer

Hibi

Westra

Borges

et al. 1999

The American Journal of Pathology

157

119

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PGP9.5 is a neurospecific peptide that functions to remove ubiquitin from ubiquitinated proteins and prevents them from targeted degradation by proteasomes. Using the serial analysis of gene expression method (SAGE) , we observed that the PGP9.5 transcript was highly expressed in primary lung cancers and lung cancer cell lines but was not detectable in the normal lung. Here we examined the expression of PGP9.5 protein in normal lung epithelium , lung tumor cell lines , and 98 resected primary non-small-cell lung carcinomas (NSCLCs). We found PGP9.5 reactivity in normal lung in a pattern compatible with Kcells of the diffuse neuroendocrine system. However, the PGP9.5 was present in both small-cell lung cancer (SCLC) and NSCLC cell lines ( Lung cancer is the second most common malignancy worldwide and is the leading cause of cancer death in men.

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“…At 10.5 days and 14 days of gestation PGP9.5 was demonstrated at low to moderate leves in the endodermal and mesodermal tissues, such as testis and bronchi. Although the distribution of PGP9.5 mRNA in developing gonads in the mouse was reported by Schofield et al [14], this is the first description of the expression of PGP9.5 protein in the developing mouse embryo. The distribution of the protein in the developing lung was reported in human and rat [2,16].…”

mentioning

confidence: 56%

“…The protein gene product 9.5 (PGP9.5) is equiva- Immunohistochemical studies have demonstrated that PGP9.5 is highly expressed in neuronal and neuroendocrine tissues [3,6,10,15,24,25], and PGP9.5 has also been shown to be expressed in the distal tubular epithelial cells, ova and corpus luteum cells of the ovary, Leydig cells and Sertoli cells of the testis of the mouse [8,22], synoviocytes in the joints of the horse [7], and hair follicle cells of the rat [11]. Localization of PGP9.5 in the mouse embryo has also been described [6,14]. PGP9.5 is present at high levels in the neuronal tissues in mouse embryo after 10.5 days of gestation [19], and in rat embryos after 11.5 days of gestation [6].…”

mentioning

confidence: 99%

Immunohistochemical Analysis of Protein Gene Product 9.5, a Ubiquitin Carboxyl-terminal Hydrolase, during Placental and Embryonic Development in the Mouse.

et al. 2003

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Protein gene product 9.5 (PGP9.5) is expressed at high level in the neural and neuroendocrine systems. We investigated the localization and degree of expression of PGP9.5 in the developing mouse placenta and embryo at 6.5, 10.5 and 14 days of gestation using an immunohistochemical technique. At 6.5 days of gestation PGP9.5 was detected at various levels in decidual and primary trophoblast giant cells in the placenta, and in embryonic ectodermal cells in the embryo. At 10.5 and 14 days of gestation PGP9.5 was expressed at moderate to strong levels in neurons in the embryo, but rarely in the placenta. These findings suggest that the protein may play a significant role in implantation and placental development, and differentiation of embryonic ectoderm.

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PGP9.5, a ubiquitin C-terminal hydrolase; pattern of mRNA and protein expression during neural development in the mouse

Cited by 87 publications

References 28 publications

Analyzing Somatosensory Axon Projections with the Sensory Neuron-SpecificAdvillinGene

Analyzing Somatosensory Axon Projections with the Sensory Neuron-SpecificAdvillinGene

PGP9.5 As a Candidate Tumor Marker for Non-Small-Cell Lung Cancer

Immunohistochemical Analysis of Protein Gene Product 9.5, a Ubiquitin Carboxyl-terminal Hydrolase, during Placental and Embryonic Development in the Mouse.

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