PGC-1.ALPHA. mRNA Level and Oxidative Capacity of the Plantaris Muscle in Rats with Metabolic Syndrome, Hypertension, and Type 2 Diabetes

Nagatomo, Fumiko; Fujino, Hidemi; Kondo, Hiroyo; Gu, Ning; Takeda, Isao; Ishioka, Noriaki; Tsuda, Kinsuke; Ishihara, Akihiko

doi:10.1267/ahc.10041

Cited by 31 publications

(50 citation statements)

References 38 publications

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“…We have previously reported that the soleus muscles in rats with type 2 diabetes contain lower levels of PGC-1a mRNA than those in non-diabetic rats [16,17]. Therefore, we concluded that low PGC-1a mRNA levels in the soleus muscles in rats with type 2 diabetes are associated with a low percentage of high-oxidative fibers.…”

Section: Introductionmentioning

confidence: 65%

The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome

et al. 2012

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Skeletal muscles in animals with metabolic syndrome exhibit reduced oxidative capacity. We investigated the effects of running exercise on fiber characteristics, oxidative capacity, and mRNA levels in the soleus muscles of rats with metabolic syndrome [SHR/NDmcr-cp (cp/cp); CP]. We divided 5-week-old CP rats into non-exercise (CP) and exercise (CP-Ex) groups. Wistar-Kyoto rats (WKY) were used as the control group. CP-Ex rats were permitted voluntary exercise on running wheels for 10 weeks. Triglyceride levels were higher and adiponectin levels lower in the CP and CP-Ex groups than in the WKY group. However, triglyceride levels were lower and adiponectin levels higher in the CP-Ex group than in the CP group. The soleus muscles in CP-Ex rats contained only high-oxidative type I fibers, whereas those in WKY and CP rats contained type I, IIA, and IIC fibers. Muscle succinate dehydrogenase (SDH) activity was higher in the CP-Ex group than in the CP group; there was no difference in SDH activity between the WKY and CP-Ex groups. Muscle proliferator-activated receptor γ coactivator-1α (PGC-1α) mRNA levels were higher in the CP-Ex group than in the CP group; there was no difference in PGC-1α mRNA levels between the WKY and CP-Ex groups. In CP-Ex rats, longer running distance was associated with increased muscle SDH activity and PGC-1α mRNA levels. We concluded that running exercise restored decreased muscle oxidative capacity and PGC-1α mRNA levels and improved hypertriglyceridemia in rats with metabolic syndrome.

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Section: Introductionmentioning

confidence: 65%

The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome

et al. 2012

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“…All samples and nontemplate control reactions were performed in a 7500 Fast Sequence Detection System (Applied Biosystems). The mRNA levels were normalized to the control (WR) group 34, 35) .…”

Section: Methodsmentioning

confidence: 99%

Mild Hyperbaric Oxygen Inhibits Growth-related Decrease in Muscle Oxidative Capacity of Rats with Metabolic Syndrome

Takemura

Ishihara

2017

JAT

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Aim: We examined the effects of mild hyperbaric oxygen on the properties of the soleus muscle in rats with metabolic syndrome.Methods: Five-week-old metabolic syndrome (SHR/NDmcr-cp, cp/cp) rats were divided into normobaric (CP) and mild hyperbaric oxygen (CP-H) groups (n = 5/group). In addition, 5-week-old Wistar rats were assigned as the normobaric control (WR) group (n = 5). The CP-H group was exposed to 1.25 atmospheres absolute with 36% oxygen for 3 h daily for 16 weeks. Succinate dehydrogenase (SDH) activity and mRNA levels of peroxisome proliferator-activated receptor γ coactivator-1α (Pgc-1α) in the soleus muscle were examined. The fiber type composition, cross-sectional areas, and SDH staining intensity in the soleus muscle were also examined.Results: The CP-H group showed lower fasting and nonfasting blood glucose, glycated hemoglobin, total cholesterol, triglyceride, insulin, and systolic blood pressure levels; higher adiponectin levels; and higher SDH activity and mRNA levels of Pgc-1α in the muscle than the CP group. Compared with the CP group, the CP-H group had a lower percentage of type I fibers and observed type IIA fibers in the muscle. The CP-H group also had higher SDH staining intensity of type I and type IIC fibers in the muscle than the CP group. No differences in these values were observed in the muscles of the WR and CP-H groups.Conclusion: Mild hyperbaric oxygen inhibited growth-related increase in blood glucose levels and decrease in muscle oxidative capacity of rats with metabolic syndrome because of improved oxidative metabolism.

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“…4E) and PGC-1α (Fig. 4F) mRNA levels than that of normal rats 44) . The plantaris muscle of GK rats exhibits lower PGC-1α mRNA levels 44) (Fig.…”

Section: Gene Expression Levels In Skeletal Musclementioning

confidence: 82%

“…PGC-1α mRNA levels in the vastus lateralis muscle are lower in patients with type 2 diabetes than in healthy individuals 62) . Lower PGC-1α mRNA levels in the soleus and plantaris muscles of GK rats are associated with lower oxidative capacity in those muscles and a fiber profile comprising only lowoxidative type I fibers in the soleus muscle 33,44) . It is concluded that reduced PGC-1α mRNA levels in the skeletal muscles of rats with type 2 diabetes are associated with a lower proportion of high-oxidative fibers.…”

Section: Gene Expression Levels In Skeletal Musclementioning

confidence: 96%

“…Obese Zucker rats with type 2 diabetes exhibit a lower proportion of type I fibers in the soleus muscle than lean Zucker rats, whereas obese Zucker rats without diabetes exhibit normal fiber type distribution in the soleus muscle 43) . The deep regions in the plantaris muscle of SHR and GK rats exhibit a lower proportion of high-oxidative type I and IIA fibers and a higher proportion of low-oxidative type IIB fibers than those of normal and CP rats 44) (Fig. 2).…”

Section: Skeletal Muscle Characteristics Of Animal Modelsmentioning

confidence: 96%

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Lifestyle-related disease and skeletal muscle: A review

Ishihara

Nagatomo

Fujino

et al. 2012

JPFSM

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The skeletal muscles in animal models with metabolic syndrome and lifestyle-related diseases, e.g., type 2 diabetes, have decreased oxidative capacity, which is associated with reduced expression levels of the gene for peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α). Running restores decreased oxidative capacity in the skeletal muscles of rats with metabolic syndrome and type 2 diabetes, and hypertriglyceridemia and hyperglycemia are improved, respectively. Longer running distances are associated with increased oxidative capacity and PGC-1α mRNA levels in the skeletal muscles of rats with metabolic syndrome and type 2 diabetes. In contrast, a high-fat diet reduces the muscle oxidative capacity and PGC-1α mRNA levels of rats with metabolic syndrome, and weight gain, hypertriglyceridemia, and hyperglycemia are accelerated. Recently, mild hyperbaric oxygen at 1.25 atmospheres absolute with 36% oxygen has been proven effective for hypertension and type 2 diabetes. Mild hyperbaric oxygen inhibits a growth-related increase in glucose levels and further decreases high glucose levels in adult rats with type 2 diabetes-changes that are associated with improvements in muscle oxidative capacity and PGC-1α mRNA levels. This paper is a review of the fiber type distribution, oxidative enzyme activity, mRNA levels, and capillary architecture of skeletal muscles in animal models with metabolic syndrome and lifestyle-related diseases. In addition, the effects of a high-fat diet, exercise, and mild hyperbaric oxygen have been summarized.

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PGC-1.ALPHA. mRNA Level and Oxidative Capacity of the Plantaris Muscle in Rats with Metabolic Syndrome, Hypertension, and Type 2 Diabetes

Cited by 31 publications

References 38 publications

The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome

The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome

Mild Hyperbaric Oxygen Inhibits Growth-related Decrease in Muscle Oxidative Capacity of Rats with Metabolic Syndrome

Lifestyle-related disease and skeletal muscle: A review

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