PFMG1 promotes osteoblast differentiation and prevents osteoporotic bone loss

Li, Liyuan; Wang, Pan; Hu, Kaiqiang; Wang, Xiaoyan; Cai, Wen; Ai, Chao; Liu, Shangfeng; Wang, Zhao

doi:10.1096/fj.201700422r

Cited by 10 publications

(12 citation statements)

References 60 publications

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“…The transcriptome or RNA expression levels in these organisms are also being explored with the goal of understanding the timing of transcription, translation, and protein secretion as it pertains to the events occurring in the biomineralizing matrix . What has emerged from these studies…”

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

“…As an example, consider the PFMG (Pinctada fucata mantle gene) protein family of the Japanese pearl oyster, Pinctada fucata , which consists of 12 different protein sequences . As of this writing, not all of the 12 proteins have been characterized, but some proteins, such as PFMG1, PFMG2, PFMG3, PFMG4, have been demonstrated to enhance cell differentiation in bone cells and hence may function as communicators which activate the mantle organ to re‐initiate the biomineralization process. Conversely, PFMG5 inhibits cell differentiation and thus may serve as a signaling agent or communicator to end the pearl formation process.…”

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

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The Biomineralization Proteome: Protein Complexity for a Complex Bioceramic Assembly Process

Evans

2019

Proteomics

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There are over 62 different biominerals on Earth and a diverse array of organisms that generate these biominerals for survival. This review will introduce the process of biomineralization and the current understanding of the molecular mechanisms of mineral formation, and then comparatively explore the representative secretomes of two well-documented skeletal systems: vertebrate bone (calcium phosphate) and invertebrate mollusk shell (calcium carbonate). It is found that both skeletal secretomes have gross similarities and possess proteins that fall into four functional categories: matrix formers, nucleation assisters, communicators, and remodelers. In many cases the mineral-associated matrix former and nucleation assister sequences in both skeletal systems are unique and possess interactive conserved globular domains, intrinsic disorder, post-translational modifications, sequence redundancy, and amyloid-like aggregation-prone sequences. Together, these molecular features create a protein-based environment that facilitates mineral formation and organization and argue in favor of conserved features that evolve from the mollusk shell to bone. Interestingly, the mollusk shell secretome appears to be more complex compared to that of bone tissue, in that there are numerous protein subcategories that are required for the nucleation and organization of inner (nacre) and outer (prismatic) calcium carbonate regions of the shell. This may reflect the organizational and material requirements of an exoskeletal protective system.

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Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

Section: Biomineral‐associated Secretomesmentioning

confidence: 99%

The Biomineralization Proteome: Protein Complexity for a Complex Bioceramic Assembly Process

Evans

2019

Proteomics

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“…Osteoblasts are critical components of the bone multicellular unit and play an important role in bone remodeling, which is essential for maintaining the structural integrity and metabolic capacity of the skeleton (47). The accumulation of osteoclasts increases the osteoclast effect, which promotes bone metabolism and reduces bone formation, and ultimately affects the bone mass and density of the vertebral body (48,49). In the elderly, vertebral body degeneration, for instance during osteoporosis or upon osteoporotic fractures, is caused by the deregulation of bone metabolism due to the imbalance of osteoblasts and osteoclasts.…”

Section: Postn Is Involved In Regulating Vertebral Body Bone Mass and Densitymentioning

confidence: 99%

Periostin: An Emerging Molecule With a Potential Role in Spinal Degenerative Diseases

Zhu

Zhou²,

Wang³

et al. 2021

Front. Med.

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Periostin, an extracellular matrix protein, is widely expressed in a variety of tissues and cells. It has many biological functions and is related to many diseases: for example, it promotes cell proliferation and differentiation in osteoblasts, which are closely related to osteoporosis, and mediates cell senescence and apoptosis in chondrocytes, which are involved in osteoarthritis. Furthermore, it also plays an important role in mediating inflammation and reconstruction during bronchial asthma, as well as in promoting bone development, reconstruction, repair, and strength. Therefore, periostin has been explored as a potential biomarker for various diseases. Recently, periostin has also been found to be expressed in intervertebral disc cells as a component of the intervertebral extracellular matrix, and to play a crucial role in the maintenance and degeneration of intervertebral discs. This article reviews the biological role of periostin in bone marrow-derived mesenchymal stem cells, osteoblasts, osteoclasts, chondrocytes, and annulus fibrosus and nucleus pulposus cells, which are closely related to spinal degenerative diseases. The study of its pathophysiological effects is of great significance for the diagnosis and treatment of spinal degeneration, although additional studies are needed.

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“…( 13 ) Furthermore, several nacre matrix factors have been identified and known to have biological activities on bone cells. Protein PFMG1 ( 14 ) and water/ethanol‐soluble matrix ( 15 , 16 ) have been derived from Pinctada 's pearl oyster family, which accelerate osteoblast differentiation. Beside the regulation of mineralization, protein N16 ( 17 ) and other molecules ( 18 ) from nacre have been demonstrated to inhibit osteoclastogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…The preclinical studies revealed that nacreous factors prevent induced bone loss. ( 8 , 14 ) Most of these studies have been conducted in ovariectomized mouse model under oral administration (gavage) or virus injection techniques, whereas our experimental powders were integrated in dietary foods, which is considered as a classic nutritional supplementation in human.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect on Bone of Nacre Supplementation in Ovariectomized Rats

et al. 2022

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Nacre has emerged as a beneficial natural product for bone cells and tissues, but its effect was only studied by gavage in the ovariectomized mouse model. We sought to assess the antiosteoporotic effect of nacre through a nutritional supplementation in the ovariectomized rat model. Sixteen‐week‐old female Wistar rats were either Sham‐operated or bilateral ovariectomized (OVX) and then fed with standard diet (Sham and OVX groups) or standard diet supplemented with either 0.25% CaCO 3 or nacre (OVX CaCO 3 and OVX Nacre group, respectively) for 28 days ( n = 10/group). The bone microarchitecture was assessed at appendicular and axial bones by micro‐computed tomography (μCT). Histomorphometric analysis was performed to determine cellular and dynamic bone parameters. Bone metabolism was also evaluated by biochemical markers and gene expression levels. Nacre‐based diet prevented the OVX‐induced bone loss better than that of the CaCO 3 supplement, given the significant changes in trabecular bone volume fraction (BV/TV) both at the femoral distal metaphysis (difference, 35%; p = 0.004) and at the second lumbar spine (difference, 11%; p = 0.01). Trabecular osteoclast surfaces (Oc.S/BS) were also 1.5‐fold lower at the tibial proximal metaphysis in OVX Nacre group compared with OVX CaCO 3 group ( p = 0.02) . By principal component analysis (PCA), OVX Nacre group formed a cluster away from OVX group and with a trend closest to Sham group. These data were consistent with biological measurements demonstrating a positive profile related to nacre supplementation, which blunted an increase in serum CTX level and enhanced serum P1NP secretion 14 days post‐OVX compared with CaCO 3 supplementation. Bmp2 mRNA expression in OVX Nacre group was +1.76‐fold ( p = 0.004) and +1.30‐fold ( p = 0.20) compared with OVX and OVX CaCO 3 groups, respectively. We conclude that supplementation with nacre could effectively limit bone loss induced by estrogen deficiency just after OVX in rats by modulating the negative imbalance of bone turnover. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

show abstract

PFMG1 promotes osteoblast differentiation and prevents osteoporotic bone loss

Cited by 10 publications

References 60 publications

The Biomineralization Proteome: Protein Complexity for a Complex Bioceramic Assembly Process

The Biomineralization Proteome: Protein Complexity for a Complex Bioceramic Assembly Process

Periostin: An Emerging Molecule With a Potential Role in Spinal Degenerative Diseases

Protective Effect on Bone of Nacre Supplementation in Ovariectomized Rats

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