pFiD188, the linear virulence plasmid of Rhodococcus fascians strain D188

Francis, Isolde M.; Keyser, Annick De; Backer, Philippe De; Simón‐Mateo, Carmen; Kalkus, Jutta; Pertry, Ine; Ardiles-Diaz, Wilson; Rycke, Riet De; Vandeputte, Olivier M.; Holsters, Marcelle Maria; Jaziri, Mondher El; Vereecke, Danny

doi:10.1094/mpmi-08-11-0215-r

Cited by 5 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, preliminary data indicate that the cytokinin profile produced by S. turgidiscabies is very different from that of R. fascians (our unpublished data), implying that the biochemistry of the Fas proteins in both organisms differs and that leafy galls can be established by different cytokinin mixes. In both organisms, the fas operon is located either in a unique region of a conjugative linear plasmid (Francis et al ., ) or on a mobilisable pathogenicity island on the chromosome (Joshi & Loria, ); therefore, it has seemingly been acquired via horizontal gene transfer. However, as long as no other organisms are identified that carry a fas operon as well, its origin will remain elusive.…”

Section: How Unique Is the R Fascians Pathology?mentioning

confidence: 99%

“…Analysis of pFiD188 revealed that it is characterized by a composite structure ( Fig. 2): the four regions that are syntenic with linear plasmids of Rhodococcus erythropolis, Rhodococcus jostii, and Rhodococcus opacus (R regions) are implicated in plasmid maintenance and dispersal, whereas the three regions that are unique for R. fascians (U regions) are involved in virulence and interaction with the host (Francis et al, 2012). The unique region U1 contains the best-studied genetic determinants in R. fascians: the attenuation (att) and the fasciation (fas) operons (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…The occurrence of linear plasmids within the actinomycetes is common, and moreover, it is not unusual that the main virulence determinants of pathogens are located on plasmids. However, hitherto, R. fascians is the only pathogen that has a linear virulence plasmid (Francis et al, 2012). Among the plant pathogens, not many induce the formation of tissue hyperplasia.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The leafy gall syndrome induced byRhodococcus fascians

Stes

Francis

Pertry

et al. 2013

FEMS Microbiol Lett

Self Cite

View full text Add to dashboard Cite

The Actinomycete Rhodococcus fascians causes the leafy gall syndrome, an infectious plant disease that affects a wide range of plants, primarily dicotyledonous herbs. The syndrome is associated with delayed senescence, loss of apical dominance, activation of dormant axillary meristems, and formation of multiple inflorescences, leading to a stunted and bushy plant appearance. A major breakthrough in the elucidation of the virulence strategy of this pathogen was the discovery of a linear virulence plasmid, pFiD188 for R. fascians strain D188. Upon perception of a compatible host plant, an autoregulatory mechanism mediated by the att operon directs a switch in the bacterial life style from a harmless epiphyte into a pathogenic endophyte and, concomitantly, activates gene expression of the fas operon that encodes a cytokinin biosynthesis pathway. A mixture of five cytokinins determines the cytokinin activity of R. fascians that directly affects plant responses and development. Moreover, the bacterial cytokinins stimulate the host to produce auxins and polyamines, that function as accessory signals to aid in symptom development. The plant reacts against the developmental hijacking by R. fascians by activating a set of counteracting measures that ultimately results in a delicate balance, allowing a long-lasting biotrophic interaction.

show abstract

Section: How Unique Is the R Fascians Pathology?mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The leafy gall syndrome induced byRhodococcus fascians

Stes

Francis

Pertry

et al. 2013

FEMS Microbiol Lett

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other bacterial genera with linear plasmids include Streptomyces and Rhodococcus species (5,6). Some of the larger plasmids of these genera have been well characterized and shown to be occupied largely by genes involved in antibiotic production and metabolic processes (7,8). Comparatively less is known about the linear plasmids of Borrelia species.…”

mentioning

confidence: 99%

Large Linear Plasmids of Borrelia Species That Cause Relapsing Fever

et al. 2013

View full text Add to dashboard Cite

c Borrelia species of relapsing fever (RF) and Lyme disease (LD) lineages have linear chromosomes and both linear and circular plasmids. Unique to RF species, and little characterized to date, are large linear plasmids of ϳ160 kb, or ϳ10% of the genome. By a combination of Sanger and next-generation methods, we determined the sequences of large linear plasmids of two New World species: Borrelia hermsii, to completion of its 174-kb length, and B. turicatae, partially to 114 kb of its 150 kb. These sequences were then compared to corresponding sequences of the Old World species B. duttonii and B. recurrentis and to plasmid sequences of LD Borrelia species. The large plasmids were largely colinear, except for their left ends, about 27 kb of which was inverted in New World species. Approximately 60% of the B. hermsii lp174 plasmid sequence was repetitive for 6 types of sequence, and half of its open reading frames encoded hypothetical proteins not discernibly similar to proteins in the database. The central ϳ25 kb of all 4 linear plasmids was syntenic for orthologous genes for plasmid maintenance or partitioning in Borrelia species. Of all the sequenced linear and circular plasmids in Borrelia species, the large plasmid's putative partition/replication genes were most similar to those of the 54-kb linear plasmids of LD species. Further evidence for shared ancestry was the observation that two of the hypothetical proteins were predicted to be structurally similar to the LD species' CspA proteins, which are encoded on the 54-kb plasmids.

show abstract

“…Streptomyces hosts carrying the linear plasmids pKSL, pSLA2-L, pSCL1 and SCP1 have been found to carry genes encoding echinomycin, lankacidins, clavulanic acid and methylenomycin, consecutively. Linear plasmids belonging to this class have also been reported in actinomycetes Planobispora, Rhodococcus, Mycobacterium and Salmonella enterica serovar Typhi (15)(16)(17)(18)(19)(20)(21)(22).…”

mentioning

confidence: 98%

Linear plasmids and their replication

Ahsan

Kabir

2013

S.J. Microbiol.

View full text Add to dashboard Cite

It is still a common belief that plasmids are circular. However, linear plasmids have been reported to exist more than a decade ago. Two types of linear plasmids are known. One type contains covalently closed ends and are commonly found in Borrelia, the causative agent of tick fever. The other type is characterized by the covalent attachment of proteins at the 5' ends and exists in a number of bacterial genera including Streptomyces, Rhodococcus, Mycobacterium and Planobispora. Recently, a linear plasmid in Salmonella enterica serovar Typhi of the Enterobacteriaceae family have been reported for the first time. This paper reviews various postulated mechanisms of replication of linear plasmids and focuses on the components of the replication machinery of linear plasmids studied to date.

show abstract

pFiD188, the linear virulence plasmid of Rhodococcus fascians strain D188

Cited by 5 publications

References 0 publications

The leafy gall syndrome induced byRhodococcus fascians

The leafy gall syndrome induced byRhodococcus fascians

Large Linear Plasmids of Borrelia Species That Cause Relapsing Fever

Linear plasmids and their replication

Contact Info

Product

Resources

About