Abstract:Background: PEX5 binds newly synthesized peroxisomal proteins in the cytosol and releases them in the organelle matrix. Results: PEX5 binds monomeric catalase and releases it in the presence of PEX14. Conclusion: PEX14 participates in the cargo release step. Significance: Knowing how PEX5 interacts with cargo proteins and which factors disrupt this interaction are crucial for understanding this protein sorting pathway.
“…Interestingly, a stable subcomplex consisting of Pex5 and Pex14 with a 1:5 stoichiometry could be isolated from rat liver peroxisomes (6). Finally, Pex14 binding to the downstream-located WXXX(F/Y) motifs six and seven triggers cargo release (10). Pex13 might also play a critical role in pore assembly or disassembly because it was shown that WXXX(F/Y) motifs 2-4 of Pex5 are able to interact with this peroxin (13).…”
Section: Discussionmentioning
confidence: 99%
“…For the model organism Saccharomyces cerevisiae, it was shown that Pex14 together with Pex5 constitutes a large dynamic channel at the peroxisomal membrane, which is supposed to act as a protein conducting pore (9). Recent data suggest that the Pex14-Pex5 interaction also plays a critical role for release of the cargo protein into the lumen of the peroxisome (10).…”
Section: In Human Cells This Interaction Is Mediated By Seven Consermentioning
confidence: 99%
“…In addition, Pex5 Val-63 is involved in hydrophobic interactions with Pex14 Val-41 and Phe-35. The con- served Val-41 is centrally located in the short 3 10 -helix of Pex14-NTD between helices ␣1 and ␣2 and makes contact with both Val-63 and Phe-66 in the LVXEF motif of Pex5 (Fig. 3C).…”
Section: Nmr Analysis and Structure Of The Complex Of Pex14-ntdmentioning
“…Interestingly, a stable subcomplex consisting of Pex5 and Pex14 with a 1:5 stoichiometry could be isolated from rat liver peroxisomes (6). Finally, Pex14 binding to the downstream-located WXXX(F/Y) motifs six and seven triggers cargo release (10). Pex13 might also play a critical role in pore assembly or disassembly because it was shown that WXXX(F/Y) motifs 2-4 of Pex5 are able to interact with this peroxin (13).…”
Section: Discussionmentioning
confidence: 99%
“…For the model organism Saccharomyces cerevisiae, it was shown that Pex14 together with Pex5 constitutes a large dynamic channel at the peroxisomal membrane, which is supposed to act as a protein conducting pore (9). Recent data suggest that the Pex14-Pex5 interaction also plays a critical role for release of the cargo protein into the lumen of the peroxisome (10).…”
Section: In Human Cells This Interaction Is Mediated By Seven Consermentioning
confidence: 99%
“…In addition, Pex5 Val-63 is involved in hydrophobic interactions with Pex14 Val-41 and Phe-35. The con- served Val-41 is centrally located in the short 3 10 -helix of Pex14-NTD between helices ␣1 and ␣2 and makes contact with both Val-63 and Phe-66 in the LVXEF motif of Pex5 (Fig. 3C).…”
Section: Nmr Analysis and Structure Of The Complex Of Pex14-ntdmentioning
“…Although it has been shown that the C-terminal half of PEX5 has a crucial role in the interaction with the PTS1 (13,14), it is becoming increasingly apparent that the N-terminal half of PEX5 also contributes to the interaction with cargo proteins (16 -20). Interestingly, recent data suggest that PEX5 may also act as a chaperone/holdase at least for some peroxisomal matrix proteins (16).…”
Background:The mammalian deubiquitinase that hydrolyzes the ubiquitin-PEX5 thioester conjugate was unknown. Results: USP9X was found to be the most active deubiquitinase acting on ubiquitin-PEX5.
Conclusion:We propose that USP9X participates in the PEX5-mediated peroxisomal protein import pathway. Significance: The unbiased biochemical strategy described here will be useful to identify deubiquitinases acting on other substrates.
“…However, the import machinery prefers monomeric proteins (Freitas et al, 2015), and PEX5 binding to catalase (Freitas et al, 2011), acyl-CoA oxidase1, and urate oxidase (Freitas et al, 2015) prevents oligomerization of these cargo proteins.…”
Section: Matrix Protein Import: Cycling Receptorsmentioning
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