Petrol and diesel exhaust particles accelerate the horizontal transfer of plasmid-mediated antimicrobial resistance genes

Zhang, Ye; Gu, April Z.; Cen, Tianyu; Li, Xiangyang; Li, Dan; Chen, Jianmin

doi:10.1016/j.envint.2018.02.038

Cited by 53 publications

(42 citation statements)

References 48 publications

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“…The size, morphology of NMOs, and impact of NMOs on cell structures and morphology was evaluated by observing the interaction of NMOs and the cells via previous procedures (Qiu et al, 2012; Zhang et al, 2018), and the detailed protocols are presented in Text S2.…”

Section: Methodsmentioning

confidence: 99%

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Nano-metal oxides induce antimicrobial resistance via radical-mediated mutagenesis

Zhang

Xie

et al. 2018

Environment International

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The widespread use of nanoparticles has triggered increasing concern and interest due to the adverse effects on global public health and environmental safety. Whether the presence of nano-metal oxides (NMOs) could facilitate the formation of new antimicrobial resistance genes (ARGs) via de novo mutation is largely unknown. Here, we proved that two widely used NMOs could significantly improve the mutation frequencies of CIP- and CHL-resistant E. coli isolates; however, the corresponding metal ions have weaker effects. Distinct concentration-dependent increases of 1.0–14.2 and 1.1–456.3 folds were observed in the resistance mutations after treatment with 0.16–100 mg/L nano-Al2O3 and 0.16–500 mg/L nano-ZnO, respectively, compared with those in the control. The resistant mutants showed resistance to multiple antibiotics and hereditary stability after sub-culturing for 5 days. We also explored the mechanism underlying the induction of antimicrobial resistance by NMOs. Whole-genome sequencing analysis showed that the mutated genes correlated with mono- and multidrug resistance, as well as undetected resistance to antibiotics. Furthermore, NMOs significantly promoted intracellular reactive oxygen species (ROS), which would lead to oxidative DNA damage and an error-prone SOS response, and consequently, mutation rates were enhanced. Our findings indicate that NMOs could accelerate the mutagenesis of multiple-antibiotic resistance and expanded the understanding of the mechanisms in nanoparticle-induced resistance, which may be significant for guiding the production and application of nanoparticles.

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Section: Methodsmentioning

confidence: 99%

“…The intracellular ROS levels in the E. coli were assessed by staining with dye 2′,7′‑Dichlorofluorescein diacetate (DCFH-DA) (Invitrogen; Carlsbad, CA). The detailed protocols and data analysis were according to our previous publications (Zhang et al, 2017, 2018) and are shown in Text S3.…”

Section: Methodsmentioning

confidence: 99%

Nano-metal oxides induce antimicrobial resistance via radical-mediated mutagenesis

Zhang

Xie

et al. 2018

Environment International

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“…Recent in vitro studies show that subinhibitory concentration of disinfectants (as present in drinkable water) such as chlorine, chloramine or hydrogen peroxide [56] and heavy metals [57] foster the conjugative transfer of plasmid holding multiple antibiotic resistance genes. Petrol and diesel exhaust particles also accelerate in vitro transfer of plasmids carrying antibiotic resistance genes [58]. In addition, a recent study shows that microplastic particles also promote the transfer of antibiotic resistance genes in microcosm experiments [59].…”

Section: Food Industry Associated Microorganismsmentioning

confidence: 99%

Potential impacts of horizontal gene transfer on human health and physiology and how anthropogenic activity can affect it

Boto

Pineda

2019

The FEBS Journal

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Horizontal gene transfer (HGT) is widespread among prokaryotes driving their evolution. In this paper, we review the potential impact in humans of the HGT between prokaryotes living in close association with humans in two scenarios: horizontal transfer in human microbiomes and transfer between microbes living in human managed environments. Although our vision is focused on the possible impact of these transfers in the propagation of antibiotic resistance genes or pathogenicity determinants, we also discuss possible human physiological adaptations via gene transfer between resident and occasional bacteria in the human microbiome.

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“…Except for the antibiotic-driven spread of ARGs (Blazquez et al, 2012;Lopatkin et al, 2016;Liu et al, 2017;Jutkina et al, 2018), non-antibiotic materials also accelerated the dissemination of plasmid-mediated ARGs (Qiu et al, 2012;Jiao et al, 2017;Zhang et al, 2018b;Cen et al, 2020). In particular, previous studies demonstrated that Cu 2+ could promote the conjugative transfer of plasmid DNA within bacterial genera (from E. coli S17-1 to E. coli K12 MG1655) (Zhang et al, 2018a) or across bacterial genera (from E. coli K-12 LE392 to P. putida KT2440) (Zhang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Copper Ions Facilitate the Conjugative Transfer of SXT/R391 Integrative and Conjugative Element Across Bacterial Genera

Song

Zuo

et al. 2021

Front. Microbiol.

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Copper can persist stably in the environment for prolonged periods. Except for inducing antibiotic resistance in bacteria, copper ions (Cu2+) can facilitate the horizontal transfer of plasmid DNA. However, whether and how Cu2+ can accelerate the conjugative transfer of SXT/R391 integrative and conjugative element (ICE) is still largely unknown. In this study, Proteus mirabilis ChSC1905, harboring an SXT/R391 ICE that carried 21 antibiotic resistance genes (ARGs), was used as a donor, and Escherichia coli EC600 was used as a recipient. Cu2+, at subinhibitory and environmentally relevant concentrations (1–10 μmol/L), significantly accelerated the conjugative transfer of SXT/R391 ICE across bacterial genera (from P. mirabilis to E. coli) (p < 0.05). The combined analyses of phenotypic tests and genome-wide sequencing indicated that reactive oxygen species (ROS) production and cell membrane permeability were critical in the enhanced conjugative transfer of SXT/R391 ICE. Furthermore, the expression of genes related to cell adhesion and ATP synthesis was also significantly upregulated on exposure to Cu2+ at a concentration of 5 μmol/L. This study clarified the potential mechanisms of Cu2+ to promote the conjugative transfer of SXT/R391 ICE, revealing the potential risk imposed by Cu2+ on the horizontal transfer of SXT/R391 ICE-mediated ARGs.

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Petrol and diesel exhaust particles accelerate the horizontal transfer of plasmid-mediated antimicrobial resistance genes

Cited by 53 publications

References 48 publications

Nano-metal oxides induce antimicrobial resistance via radical-mediated mutagenesis

Nano-metal oxides induce antimicrobial resistance via radical-mediated mutagenesis

Potential impacts of horizontal gene transfer on human health and physiology and how anthropogenic activity can affect it

Copper Ions Facilitate the Conjugative Transfer of SXT/R391 Integrative and Conjugative Element Across Bacterial Genera

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