PET/CT with [68Ga]gallium-oxine-labeled heat-denatured red blood cells for detection of dystopic splenic tissue

Freesmeyer, Martin; Gröber, Sebastian; Greiser, Julia; Seifert, Philipp; Gühne, Falk; Drescher, Robert

doi:10.1007/s00259-020-04899-4

Cited by 9 publications

(6 citation statements)

References 4 publications

(2 reference statements)

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“…However, [ 68 Ga]Ga-oxine has limited use for cell tracking applications that require long imaging timeframes because of the short half-life of 68 Ga (68 min). Nevertheless, [ 68 Ga]Ga-oxine was recently used clinically for the labeling and tracking of heat-denatured RBCs over short periods with clinical PET/CT imaging …”

Section: Chemical Probes For Ex Vivo Direct Cell Radiolabelingmentioning

confidence: 99%

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Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging

et al. 2022

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The arrival of cell-based therapies is a revolution in medicine. However, its safe clinical application in a rational manner depends on reliable, clinically applicable methods for determining the fate and trafficking of therapeutic cells in vivo using medical imaging techniquesknown as in vivo cell tracking. Radionuclide imaging using single photon emission computed tomography (SPECT) or positron emission tomography (PET) has several advantages over other imaging modalities for cell tracking because of its high sensitivity (requiring low amounts of probe per cell for imaging) and whole-body quantitative imaging capability using clinically available scanners. For cell tracking with radionuclides, ex vivo direct cell radiolabeling, that is, radiolabeling cells before their administration, is the simplest and most robust method, allowing labeling of any cell type without the need for genetic modification. This Review covers the development and application of direct cell radiolabeling probes utilizing a variety of chemical approaches: organic and inorganic/coordination (radio)chemistry, nanomaterials, and biochemistry. We describe the key early developments and the most recent advances in the field, identifying advantages and disadvantages of the different approaches and informing future development and choice of methods for clinical and preclinical application.

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Section: Chemical Probes For Ex Vivo Direct Cell Radiolabelingmentioning

confidence: 99%

“…Heat-damaged RBCs are also used for spleen imaging (Figure B-E). [ 68 Ga]Ga-oxine was recently evaluated in the clinic for the labeling of heat-denatured RBC, helping to identify a benign splenic nodule that could otherwise have been mistaken for a metastatic lesion …”

Section: Applications and Clinical Translation Of Cell Trackingmentioning

confidence: 99%

Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging

et al. 2022

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“…6,7 PET/CT with this tracer, injected intravenously, is performed to differentiate functional splenic tissue from malignant lesions and to detect splenosis. 8 Damage (denaturation) of the erythrocytes and its labeling is done in vitro in 1 laboratory step (incubation for 10 minutes at 48°C-50°C). 9 Using the same protocol, but incubating for 10 minutes at 36°C-37°C, leads to labeled but undamaged erythrocytes, representing a suitable tracer for bleeding site detection.…”

Section: Clinical Nuclear Medicine • Volume 49 Number 3 March 2024mentioning

confidence: 99%

“…In our clinic, the synthesis of 68 Ga-oxine and the labeling of heat-damaged erythrocytes according to GMP standards have been established 6,7 . PET/CT with this tracer, injected intravenously, is performed to differentiate functional splenic tissue from malignant lesions and to detect splenosis 8 . Damage (denaturation) of the erythrocytes and its labeling is done in vitro in 1 laboratory step (incubation for 10 minutes at 48°C–50°C) 9 .…”

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confidence: 99%

68Ga-Oxine–Labeled Erythrocytes as a New PET Tracer for the Localization of Gastrointestinal Bleeding

Leder,

Kühnel,

Gröber

et al. 2023

Clin Nucl Med

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A 69-year-old man presented with recurring drops in hemoglobin levels and suspected gastrointestinal bleeding. Endoscopy did not show a site of bleeding so further examinations became necessary. Scintigraphy and SPECT/CT with 99mTcO4 −–labeled red blood cells were performed without evidence of a hemorrhage. Based on an established protocol for splenic PET/CT, autologous erythrocytes can be labeled with 68Ga-oxine and used as a tracer for the localization of active bleeding sites. In the patient, PET/CT with 68Ga-oxine–labeled undamaged erythrocytes was performed successfully and revealed a hemorrhage of the gastric corpus that was confirmed and treated by endoscopy.

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“…A trend in nuclear medicine imaging is the replacement of 99m Tc-based scintigraphy with positron emission tomography (PET)/computed tomography (CT) methods to utilize the higher spatial and temporal resolution of this modality. PET/CT with RBCs labeled with gallium-68-oxine ([ 68 Ga]Ga-8-hydroxyquinoline) was first reported in 2021 and has since been in clinical use in our institution [4,5]. We report the case of a patient in whom disseminated abdomino-pelvic splenosis was diagnosed using spleen-specific PET/CT.…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Splenic Imaging: [68Ga]Ga-Oxine Red Blood Cell PET/CT for Differentiation of Splenosis Mimicking Malignant Lymphoma

2022

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The differentiation of splenic tissue from malignant lesions via imaging may be challenging, particularly considering aberrant or accessory lesions and diseases that are rarely encountered. Functioning splenic tissue can be identified using technetium-99m red blood cell (99mTc-RBC) scintigraphy, but its sensitivity is limited and may not be available. We present the case of a patient in whom disseminated abdomino-pelvic splenosis was diagnosed using PET/CT with gallium-68-oxine-labeled RBCs. The method represents a feasible and probably superior alternative to splenic scintigraphy.

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PET/CT with [68Ga]gallium-oxine-labeled heat-denatured red blood cells for detection of dystopic splenic tissue

Cited by 9 publications

References 4 publications

Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging

Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging

68Ga-Oxine–Labeled Erythrocytes as a New PET Tracer for the Localization of Gastrointestinal Bleeding

High-Resolution Splenic Imaging: [68Ga]Ga-Oxine Red Blood Cell PET/CT for Differentiation of Splenosis Mimicking Malignant Lymphoma

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