PET and SPECT Reporter Gene Imaging

Yaghoubi, Shahriar

doi:10.1142/9789814293686_0013

Cited by 4 publications

(3 citation statements)

References 124 publications

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“…The choice of the substrates depends on their safety profile, pharmacokinetics and biodistribution. 18 F-FHBG is safe for clinical use in PET tracer doses and the combination of 18 F-FHBG and HSV1-sr39tk gives the best signal to background ratio for tracking the T cells outside the gut area 92 . Indirect labeling has been used for tracking the tumor-specific T cells in SCID mice bearing human tumor xenografts (using 124 I-FIAU or 131 I-FIAU) 93 and in nonhuman primates (using 18 F-FEAU) 89 ; detecting TCR-dependent T cell activation (using 124 I-FIAU) 94 ; and evaluating the tumor-killing activity of therapeutic cytotoxic T cells in a clinical trial (using 18 F-FHBG, Fig.…”

Section: Tracking the T Cells By Pet/spectmentioning

confidence: 99%

Molecular Imaging in Tracking Tumor-Specific Cytotoxic T Lymphocytes (CTLs)

Liu¹,

Zheng²

2014

Theranostics

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Despite the remarkable progress of adoptive T cell therapy in cancer treatment, there remains an urgent need for the noninvasive tracking of the transfused T cells in patients to determine their biodistribution, viability, and functionality. With emerging molecular imaging technologies and cell-labeling methods, noninvasive in vivo cell tracking is experiencing impressive progress toward revealing the mechanisms and functions of these cells in real time in preclinical and clinical studies. Such cell tracking methods have an important role in developing effective T cell therapeutic strategies and steering decision-making process in clinical trials. On the other hand, they could provide crucial information to accelerate the regulatory approval process on the T cell therapy. In this review, we revisit the advances in tracking the tumor-specific CTLs, highlighting the latest development in human studies and the key challenges.

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Section: Tracking the T Cells By Pet/spectmentioning

confidence: 99%

Molecular Imaging in Tracking Tumor-Specific Cytotoxic T Lymphocytes (CTLs)

Liu¹,

Zheng²

2014

Theranostics

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“…For instance, superparamagnetic iron oxides (SPIOs) as MRI trackers can ensure excellent anatomic information in deep organs, but the signal becomes ambiguous when cell numbers are low [ 22 , 23 ]. PET-CT detection is ultrasensitive, they are potential ionizing hazards, and the temporal resolution is relatively low [ 24 , 25 ]. On the other hand, fluorescence imaging enjoys the merits of high sensitivity, high temporal resolution, and excellent maneuverability, which promotes their broad applications in a variety of biomedical imaging tasks in animal models.…”

Section: Introductionmentioning

confidence: 99%

Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker

et al. 2021

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Stem cell therapy holds high promises in regenerative medicine. The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution, migration, and engraftment, which cannot be easily achieved by current techniques. To address this issue, for the first time, we have developed a molecular cell tracker with a strong fluorescence signal in the second near-infrared (NIR-II) window (1,000-1,700 nm) for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models. The NIR-II tracker (CelTrac1000) has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high spatiotemporal resolution for semiquantification of the biodistribution of transplanted stem cells. Taking advantage of the unique merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled. Furthermore, we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a 100 μm single-cell cluster spatial resolution, along with the lung contraction and heart beating. As such, this NIR-II tracker will shift the optical cell tracking into a single-cell cluster and millisecond temporal resolution for better evaluating and understanding stem cell therapy, affording optimal doses and efficacy.

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“…22,23 PET/CT detection is ultrasensitive, they are potential ionizing hazards and the temporal resolution is relatively low. 24,25 On the other hand, fluorescence imaging enjoys the merits of high sensitivity, high temporal resolution, and excellent maneuverability, which promotes their broad applications in a variety of biomedical imaging tasks in animal models. To realize in vivo, noninvasive, deep tissue fluorescence imaging with high temporal-spatial resolution, exogenous probes with emission in near-infrared (NIR) region are preferred.…”

Section: Introductionmentioning

confidence: 99%

Differential responses of transplanted stem cells to the diseased environment unveiled by a single molecular NIR II cell tracker

Chen

Yang

Zhang

et al. 2020

Preprint

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designed and conducted the probe synthesis, in vitro experiments, in vivo experiments, analyzed and interpreted data, wrote the manuscript; C.Z. M.Z. and Z.W. contributed to the manuscript writing; S.C. performed the ALI and MCAO model experiments; X.Z. carried out the MI model experiments; H.T.W. differentiated the iPSC-ECs; Z.C. provided experimental design and advice, manuscript writing, and funding support. All authors reviewed the manuscript. AbstractStem cell therapy holds high promises in regenerative medicine. The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution, migration, and engraftment, which cannot be easily achieved by current techniques. To address this issue, for the first time, we have developed a single molecular cell tracker with a strong fluorescence signal in the second near-infrared (NIR-II) window (1000-1700 nm) for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models. The NIR-II tracker (CelTrac1000) has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high temporospatial resolution for semi-quantification of the biodistribution of primary mesenchymal stem cell and induced pluripotent stem cell-derived endothelial cells. Taking advantage of the unique merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled. Furthermore, we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a single cell cluster resolution, along with the lung contraction and heart beating. As such, this single molecular NIR-II tracker will shift the optical cell tracking into a single cell cluster and millisecond temporospatial resolution for better evaluating and understanding stem cell therapy, affording optimal doses and efficacy. Significance StatementFor the first time, we synthesized a NIR-II tracker (CelTrac1000) for ultrafast real-time tracking of the migration trajectory of transplanted mesenchymal stem cells in the circulatory system with a single cell cluster resolution. Taking advantage of the merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments, including acute lung injury, myocardial infarction, and middle cerebral artery occlusion, have been discriminated and unveiled in mice models. As such, our approach can help correlate critical biomedical information in stem cell therapies, such as stem cell dosing and engraftment and their relationships with efficacy, providing more accurate therapeutic treatment and outcomes in certain diseases during a long evaluation period (>30 days) in comparison with the commercial Qtracker (7-10 days). Main Text

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PET and SPECT Reporter Gene Imaging

Cited by 4 publications

References 124 publications

Molecular Imaging in Tracking Tumor-Specific Cytotoxic T Lymphocytes (CTLs)

Molecular Imaging in Tracking Tumor-Specific Cytotoxic T Lymphocytes (CTLs)

Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker

Differential responses of transplanted stem cells to the diseased environment unveiled by a single molecular NIR II cell tracker

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