Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription

Fyodorov, Dmitry V.; Nelson, Tom; Deneris, Evan S.

doi:10.1002/(sici)1097-4695(19980205)34:2<151::aid-neu5>3.0.co;2-1

Cited by 54 publications

(18 citation statements)

References 46 publications

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“…Importantly, no X-gal staining was observed within the metanephric mesenchyme, thus suggesting that in Pet1 210 -Cre transgenic mouse line Cre-mediated recombination occurred specifically within the ureteric bud precursors. No Cre recombinase activity was detected in the adrenal gland of Pet1 210 -Cre/ROSA26R mice (Figure S4 c, i-i’) as expected in agreement with Fyodorov and collaborators (1998) [45].…”

Section: Resultssupporting

confidence: 91%

“…In good agreement with our observation that Pet1 is expressed in mammalian kidney, microarray analysis performed on mouse and rat transcriptomes indicated that Pet1 transcript was enriched in the ureteric bud as compared to other cell types in the developing kidney such as the metanephric mesenchyme [63]. Nevertheless, although Pet1 expression has been shown in the adrenal gland by Fyodorov and collaborators [45], Cre recombinase activity in this district was not detected either by Scott and collaborators [16], or from our analysis, using ePet-Cre and ePet-YFP , or Pet1 210 -Cre transgenic mouse lines, respectively. The presence of an intact recombined BAC in Pet1 210 -Cre mice suggests that the specific enhancer driving expression of Pet1 in the adrenal gland may be a long-range acting regulatory element, which is not likely present in the 210 kb genomic region contained in the RP23_165_D11 BAC clone.…”

Section: Discussionsupporting

confidence: 92%

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Generation of Pet1210-Cre Transgenic Mouse Line Reveals Non-Serotonergic Expression Domains of Pet1 Both in CNS and Periphery

et al. 2014

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Neurons producing serotonin (5-hydroxytryptamine, 5-HT) constitute one of the most widely distributed neuronal networks in the mammalian central nervous system (CNS) and exhibit a profuse innervation throughout the CNS already at early stages of development. Serotonergic neuron specification is controlled by a combination of secreted molecules and transcription factors such as Shh, Fgf4/8, Nkx2.2, Lmx1b and Pet1. In the mouse, Pet1 mRNA expression appears between 10 and 11 days post coitum (dpc) in serotonergic post-mitotic precursors and persists in serotonergic neurons up to adulthood, where it promotes the expression of genes defining the mature serotonergic phenotype such as tryptophan hydroxylase 2 (Tph2) and serotonin transporter (SERT). Hence, the generation of genetic tools based on Pet1 specific expression represents a valuable approach to study the development and function of the serotonergic system. Here, we report the generation of a Pet1210-Cre transgenic mouse line in which the Cre recombinase is expressed under the control of a 210 kb fragment from the Pet1 genetic locus to ensure a reliable and faithful control of somatic recombination in Pet1 cell lineage. Besides Cre-mediated recombination accurately occurred in the serotonergic system as expected and according to previous studies, Pet1210-Cre transgenic mouse line allowed us to identify novel, so far uncharacterized, Pet1 expression domains. Indeed, we showed that in the raphe Pet1 is expressed also in a non-serotonergic neuronal population intermingled with Tph2-expressing cells and mostly localized in the B8 and B9 nuclei. Moreover, we detected Cre-mediated recombination also in the developing pancreas and in the ureteric bud derivatives of the kidney, where it reflected a specific Pet1 expression. Thus, Pet1210-Cre transgenic mouse line faithfully drives Cre-mediated recombination in all Pet1 expression domains representing a valuable tool to genetically manipulate serotonergic and non-serotonergic Pet1 cell lineages.

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Section: Resultssupporting

confidence: 91%

Section: Discussionsupporting

confidence: 92%

Generation of Pet1210-Cre Transgenic Mouse Line Reveals Non-Serotonergic Expression Domains of Pet1 Both in CNS and Periphery

et al. 2014

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“…Therefore, the impact of their early embryonic loss of function cannot be investigated in adults until conditional gene targeting approaches are used. An exception, however, is Pet-1 whose expression is restricted to serotonin neurons and a small number of neural and non-neural cell types outside the brain (Fyodorov et al, 1998;Hendricks et al, 1999). The function of the Pet-1 gene in these cell types is not essential for viability and therefore the majority of Pet-1 null mice live to adulthood.…”

Section: Molecular Genetics Of Serotonin Neuron Developmentmentioning

confidence: 99%

Making and breaking serotonin neurons and autism

Scott

Deneris

2005

Intl J of Devlp Neuroscience

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Dysfunction of brain serotonin system development is hypothesized to contribute to autistic behaviors. The testing of this hypothesis will likely depend on a better understanding of the genes and mechanisms involved in serotonin neuron cell fate specification. In this review we summarize the main features of vertebrate serotonin neuroanatomical development and recent studies that have revealed critical steps in the molecular genetic program that controls serotonin neuron phenotype. We then discuss the potential relevance of these findings to advances in autism research and to new molecular genetic tools under development that will impact future testing of the hypothesis.

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“…1a). Intriguingly, the initiation of the translation of mPet-1 differs completely from the rat Pet-1 gene (Fyodorov et al 1998), but is identical to the equivalent position in the human FEV gene (Peter et al 1997). The predicted mouse mPet-1 protein comprises 237 amino acids displaying 96% similarity to the human FEV protein.…”

mentioning

confidence: 98%

mPet-1, a mouse ETS-domain transcription factor, is expressed in central serotonergic neurons

et al. 2002

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Here we describe the expression pattern of a previously unknown mouse gene mPet-1. The isolated cDNA codes for an ETS-domain transcription factor of 237 amino acids in length, which is localized to the nucleus. mPet-1 is a member of the winged helix transcription factor gene family like its rat homologue Pet-1 and the human homologue FEV. The start ATG of mPet-1 and the size of the predicted protein are identical to the human FEV. The mPet-1 protein is clearly smaller since it lacks the first 103 N-terminal amino acids of rat Pet-1. mPet- 1 is expressed in central serotonergic (5-hydroxytryptaminergic) neurons located in the mes-/metencephalic raphe nuclei from E11 on until adulthood. In these regions mPet-1 expression co-localizes precisely with the serotonin transporter (Sert),which it initially precedes. Interestingly, mPet-1 was not found in neurons transiently expressing Sert.

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Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription

Cited by 54 publications

References 46 publications

Generation of Pet1210-Cre Transgenic Mouse Line Reveals Non-Serotonergic Expression Domains of Pet1 Both in CNS and Periphery

Generation of Pet1210-Cre Transgenic Mouse Line Reveals Non-Serotonergic Expression Domains of Pet1 Both in CNS and Periphery

Making and breaking serotonin neurons and autism

mPet-1, a mouse ETS-domain transcription factor, is expressed in central serotonergic neurons

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