Pesudomance sp. Bacteria Associated with Marine Sponge as a Promising and Sustainable Source of Bioactive Molecules

Abstract

Background: The study was conducted to identify the bacterial strain associated with marine sponge Hyrtiosaff. erectus collected from the Red Sea coastal water and to assess the utilization of their secondary metabolites for human benefit as antioxidant, anti-Alzheimer, anti-viral, anticancer and anti-inflammatory agent. Methods: After biochemical identification of Pesudomance sp. bacterial strain, the total polyphenol contents, cytotoxic, antioxidant, anti-Alzheimer, anti-viral, anticancer and anti-inflammatory activity of the Pesudomance sp. ethyl acetate extract were investigated by applying different biochemical assays. Polyphenol contents were investigated using spectrophotometric techniques. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and 2,2/-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ABTS radical scavenging activity assays. The cytotoxic effects were investigated by using the human cancerous cell lines. Results: The anti-Alzheimer, anti-viral, anticancer and anti-inflammatory activities were determined using ELISA. Qualitative phytochemical analysis of the Pesudomance sp. extract demonstrated the presence of a large and diverse group of substances such as alkaloids, carbohydrates, flavonoids, phenols, terpenoids, saponins, and tannins. The strong antioxidant activity of the Pesudomance sp. extract was mainly attributed to the protective role of polyphenols against reactive oxygen. It was also observed that Pesudomance sp. extract possessed significant anti-Alzheimer activity with 94% at 1 mg. The extract showed also high antiviral activity (90%) using reverse transcriptase enzymes inhibition assay. The examination of the anticancer activity by applying two experimental models, i.e., PTK and SHKI cleared out high significant percentages of 76.19 and 83.09 %; respectively. Conclusion: The anti-inflammatory profiling using TNF, COX1, COX2, IL6 also revealed high antiinflammatory activity with different metabolic pathway of 62.70, 75.444, 79.27 and 54.15 %; respectively. The present study concluded that ethyl acetate extract of Pesudomance sp. possessed strong antioxidant, anti-Alzheimer, and anti-viral, anticancer and anti-inflammatory activities. Further studies are required to purify the bioactive compounds.

Red algae natural products for prevention of lipopolysaccharides (LPS)-induced liver and kidney inflammation and injuries

“…The G. oblongata marine extract was analyzed by GC-ITQ-MS using a Thermo Trace GC Ultra TM gas chromatograph system (Thermo Scientific, U.S.A.) according to the methods described by Nabil-Adam et al [ 6 ]. The identification and interpretation of G. oblongata marine extract results of mass-spectrum GC-MS were done by comparing their mass spectra with those obtained from the NIST (National Institute of Standards and Technology) mass spectral database as conducted using the database of National Institute Standard and Technology (NIST).…”

“…Marine algae are important and valuable sources of structurally diverse biomaterials having antioxidant, anti-inflammatory, anti-coagulant, and anti-thrombotic activities amongst others [4,5,6]. Because free radicals resulted from oxidative stress have important effect in inflammatory reactions process and also in carcinogenesis, the marine algal natural bioactive compounds have been representing potential for use in different area and applications such as anti-cancer, antioxidant and anti-inflammatory drugs candidates.…”

“…As a matter of fact, they produce a broad variety of unique potent substances, which include among others, carotenoids, terpenoids, xanthophylls, chlorophylls, phycobilins, polyunsaturated fatty acids, polysaccharides, vitamins etc. exhibiting potential beneficial use and stable properties for therapeutic use with low potential toxicity including antioxidant, anti-inflammatory, antiallergenic, antiaging and antiwrinkling effects and ultraviolet protection [ 5 , 6 , 25–28 ]. Moreover, the high level of minerals, vitamins, essential amino acids, indigestible carbohydrates and polyphenols, as well as their bioactive metabolites in the red algae and their relatively easy cultivation makes them attractive [ 30 ].…”

Flavonoids Biosynthesis in Plants as a Defense Mechanism: Role and Function Concerning Pharmacodynamics and Pharmacokinetic Properties

“…The major concern of several studies was the regulatory effects of polyphenolics on the human body to understand the Pharmacodynamics mechanism [99][100][101][102][103][104][105]. The current study investigates the effect of 14 polyphenolic compounds from the sponge, and the pharmacodynamics of the 14 compounds were listed and investigated according to ADME (Adsorption, Distribution, Metabolism, and Excretion).…”

Modulation of MAPK/NF-κB Pathway and NLRP3 Inflammasome by Secondary Metabolites from Red Algae: A Mechanistic Study

“…The Red Sea marine environment is a unique and rich natural resource for many bioactive substances with unique structural features due to its phenomenal biodiversity. Marine bioactive compounds, including marine plants, macro- and microalgae, microorganisms, and sponges, can be derived from various sources containing their own unique set of biomolecules. − Macroalgae, or seaweed, are found in tropical waters and intertidal regions. They are rich in interesting bioactive components, including vitamins, hormones, carotenoids, etc., showing potential positive uses in healthcare. − The study aims to investigate the protective effect of Galaxaura oblongata (G.…”