Pesticide vapor sensing using an aptamer, nanopore, and agarose gel on a chip

Fujii, Satoshi; Nobukawa, Aiko; Osaki, Toshihisa; Morimoto, Yuya; Kamiya, Kanichi; Misawa, Nobuo; Nie, Minghao

doi:10.1039/c7lc00361g

Cited by 46 publications

(37 citation statements)

References 40 publications

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“…Other research works involving a nanopore detection of a target with an aptamer in solution concerns small molecules such as ions [ 154 , 161 ], ATP [ 162 ], or sensitive compounds such as cocaine [ 156 , 159 , 163 , 164 ] or pesticides [ 165 , 166 , 167 ]. In 2011, Kawano et al presented an embedded device for a rapid aptamer-based detection of 1 µM cocaine in solution with an α-HL nanopore [ 156 , 163 ].…”

Section: Nanopores and Aptamers: A Winning Combinationmentioning

confidence: 99%

“…When the aptamer in solution is bound to its target, it is too large to go through the nanopore and clogs the entry, thus generating a specific current signature. Later, the same group has added a hydrogel to absorb and detect the organic volatile compounds from the vapor phase [ 167 ]. They exposed the hydrogel to vaporized omethoate at a concentration of 100 ppb (part per billion) for 10 min, resulting in an absorption of 600 nM vaporized omethoate and its detection with the aptamer and the nanopore.…”

Section: Nanopores and Aptamers: A Winning Combinationmentioning

confidence: 99%

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Sensing with Nanopores and Aptamers: A Way Forward

Reynaud

Bouchet-Spinelli

Raillon

et al. 2020

Sensors

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In the 90s, the development of a novel single molecule technique based on nanopore sensing emerged. Preliminary improvements were based on the molecular or biological engineering of protein nanopores along with the use of nanotechnologies developed in the context of microelectronics. Since the last decade, the convergence between those two worlds has allowed for biomimetic approaches. In this respect, the combination of nanopores with aptamers, single-stranded oligonucleotides specifically selected towards molecular or cellular targets from an in vitro method, gained a lot of interest with potential applications for the single molecule detection and recognition in various domains like health, environment or security. The recent developments performed by combining nanopores and aptamers are highlighted in this review and some perspectives are drawn.

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Section: Nanopores and Aptamers: A Winning Combinationmentioning

confidence: 99%

Section: Nanopores and Aptamers: A Winning Combinationmentioning

confidence: 99%

Sensing with Nanopores and Aptamers: A Way Forward

Reynaud

Bouchet-Spinelli

Raillon

et al. 2020

Sensors

View full text Add to dashboard Cite

show abstract

“…The reported device utilized the "traditional" cis-trans chamber configuration with an α-hemolysin nanopore embedded in an SLB separating between them. Using agarose in the cis side, which facilitated the dissolution of the volatile pesticide omethoate, and specific aptamers, blocking currents were recorded that corresponded to omethoate concentrations in the ppb range [78]. Insect ORs are attractive candidates for SLB-based nanopore sensors since they function as ligand-gated ion channels with a wide range of ligands [79].…”

Section: Suspended Lipid Bilayersmentioning

confidence: 99%

The Emergence of Insect Odorant Receptor-Based Biosensors

Bohbot

Vernick

2020

Biosensors

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The olfactory receptor neurons of insects and vertebrates are gated by odorant receptor (OR) proteins of which several members have been shown to exhibit remarkable sensitivity and selectivity towards volatile organic compounds of significant importance in the fields of medicine, agriculture and public health. Insect ORs offer intrinsic amplification where a single binding event is transduced into a measurable ionic current. Consequently, insect ORs have great potential as biorecognition elements in many sensor configurations. However, integrating these sensing components onto electronic transducers for the development of biosensors has been marginal due to several drawbacks, including their lipophilic nature, signal transduction mechanism and the limited number of known cognate receptor-ligand pairs. We review the current state of research in this emerging field and highlight the use of a group of indole-sensitive ORs (indolORs) from unexpected sources for the development of biosensors.

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“…This system has been used to measure signals from biological nanopores and various types of ion channels expressed on the plasma membrane or other organelle membranes in living cells [77]. Additionally, small-molecule single-strand DNA or miRNA has also been detected using biological nanopores [78][79][80][81][82].…”

Section: Asymmetric Lipid Vesicles Formation Using Microfluidic Technmentioning

confidence: 99%

Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology

Kamiya

2020

Micromachines

Self Cite

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Giant lipid vesicles or liposomes are primarily composed of phospholipids and form a lipid bilayer structurally similar to that of the cell membrane. These vesicles, like living cells, are 5–100 μm in diameter and can be easily observed using an optical microscope. As their biophysical and biochemical properties are similar to those of the cell membrane, they serve as model cell membranes for the investigation of the biophysical or biochemical properties of the lipid bilayer, as well as its dynamics and structure. Investigation of membrane protein functions and enzyme reactions has revealed the presence of soluble or membrane proteins integrated in the giant lipid vesicles. Recent developments in microfluidic technologies and synthetic biology have enabled the development of well-defined artificial cell models with complex reactions based on the giant lipid vesicles. In this review, using microfluidics, the formations of giant lipid vesicles with asymmetric lipid membranes or complex structures have been described. Subsequently, the roles of these biomaterials in the creation of artificial cell models including nanopores, ion channels, and other membrane and soluble proteins have been discussed. Finally, the complex biological functions of giant lipid vesicles reconstituted with various types of biomolecules has been communicated. These complex artificial cell models contribute to the production of minimal cells or protocells for generating valuable or rare biomolecules and communicating between living cells and artificial cell models.

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Pesticide vapor sensing using an aptamer, nanopore, and agarose gel on a chip

Cited by 46 publications

References 40 publications

Sensing with Nanopores and Aptamers: A Way Forward

Sensing with Nanopores and Aptamers: A Way Forward

The Emergence of Insect Odorant Receptor-Based Biosensors

Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology

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