Peste des Petits Ruminants (PPR) Outbreak in Tajikistan

Kwiatek, Olivier; Minet, Cécile; Grillet, Colette; Hurard, Corinne; Carlsson, Emil; Karimov, B.; Albina, Emmanuel; Diallo, Adama; Libeau, Geneviève

doi:10.1016/j.jcpa.2006.12.002

Cited by 137 publications

(138 citation statements)

References 28 publications

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“…High morbidity and mortality rates in young animals were also reported in other studies (Yeşilbağ et al, 2005;Albayrak and Alkan, 2009;Aytekin et al, 2011). As our observations showed (unpublished data), in young animals severity of symptoms and mortality rate were higher in PPR virus infection in the presence of other viruses such as sheep and goat pox or secondary bacterial infections (Kwiatek et al, 2007;Ozmen et al, 2009).…”

Section: Discussionsupporting

confidence: 73%

“…Identification of virus lineage is also essential for understanding epidemiology and for control of the disease. Phylogenetically, the PPR virus is classified into 4 lineages based on partial sequencing of F (Özkul et al, 2002) and N genes (Kwiatek et al, 2007;Munir et al, 2012aMunir et al, , 2012b. The lineages are generally correlated with geographic distribution of the virus (Shaila et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…PPR viruses belonging to lineages I and II have been isolated exclusively from west and central African countries including the Ivory Coast, where the PPR virus was first detected in the 1940s (Chard et al, 2008;Luka et al, 2011;Munir et al, 2012b). Lineage III has been isolated from eastern Africa and the Arabian peninsula (Shaila et al, 1996;Kwiatek et al, 2011); lineage IV has been isolated in Asia (Kwiatek et al, 2007;Kerur et al, 2008;Wang et al, 2009;Balamurugan et al, 2010;Munir et al, 2012a;Anees et al, 2013), in the Middle East (Esmaelizad et al, 2011), and more recently in northern Africa (Sanz-Alvarez et al, 2008;Kwiatek et al, 2011;De Nardi et al, 2012 locations 5737-5761 and 6184-6160, respectively (Özkul et al, 2002). The RT-PCR reaction was performed with a 1-step RT-PCR kit (QIAGEN) in a final volume of 20 µL, which contained 4 µL of 5X 1-step RT-PCR buffer, 0.4 µM of each primer, 0.8 µL of 10 mM of each dNTP, 0.8 µL enzyme mix, 4 µL of 5X Q solution, 7 µL of PCR-grade water, and 2.6 µL of sample RNA.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic analysis of peste des petits ruminants virus from outbreaks in Turkey during 2008–2012

Guler¹,

Şevik²,

Hasöksüz³

2014

Turk J Biol

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Peste des petits ruminants (PPR) is an important viral disease of sheep and goats and is endemic in all regions of Turkey. In this study, PPR virus infection was investigated by RT-PCR assay based on the fusion (F) gene in PPR-suspected sheep and goat samples. PPR virus RNA was detected in 65 small ruminants (51 sheep, 14 goats) from independent outbreaks during [2008][2009][2010][2011][2012] in provinces in the central and Mediterranean regions and the central-west part of the Aegean region in Turkey. The virus was detected in an aborted sheep fetus sample by RT-PCR, and diagnosis was also confirmed by virus isolation. Vaccine strain Nigeria 75/1 was differentiated from field isolates by restriction fragment length polymorphism analysis of RT-PCR products using EcoRI. Phylogenetic analysis of 16 viruses indicated that all viruses, including the one from the aborted sheep fetus, belonged to lineage IV, as had the PPR viruses previously isolated in Turkey. Nucleotide sequence identity among 16 viruses was 99.1%-100%. Results showed that PPR virus lineage IV has been in circulation in Turkey since the first detection of the disease.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic analysis of peste des petits ruminants virus from outbreaks in Turkey during 2008–2012

Guler¹,

Şevik²,

Hasöksüz³

2014

Turk J Biol

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“…It has negative sense single stranded RNA with the genome sized approximately 16 kilobases (kb). The PPRV is grouped into four (I-IV) genetically different lineages, which are based on the sequence variability of the carboxy terminal in the N protein gene (Kwiatek et al, 2007). Small wild ruminant species and antelope are also severely affected (Elzein et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Detection of <em>Peste des Petits Ruminants</em> Virus genome in fecal material of goats after an outbreak in Punjab Province of Pakistan: A longitudinal study

Ullah

Zahur²,

Irshad³

et al. 2015

SL J Food & Agric

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Peste des Petits Ruminants (PPR) is a highly contagious viral disease of domestic and wild small ruminants causing high morbidity and mortality (up to 100%) and thus, has serious socioeconomic implications. In Pakistan, estimated losses due to PPR during the year 2012-13 were worth Pakistan Rs. 31.51 billion. Close contact between infected and susceptible animal is an important route of transmission of PPR. Therefore, carrier animals play an important role in unnoticed transmission of the disease. Limited information is available about the persistence of PPR virus in goats. Hence, the objective of the study was to investigate the persistence of PPR virus (PPRV) in goats recovered from PPR. A suspected PPR outbreak in sub-urban area of Lahore district of the Punjab Province in Pakistan was investigated. The outbreak was confirmed as PPR after analyzing appropriate samples (nasal/ocular swabs, fecal and tissue samples) collected from the infected animals. A longitudinal study was conducted over a period of 16 weeks to ascertain the persistence of PPRV in the fecal samples of recovered goats (n=96) collected at 4, 8, 12 and 16 weeks after the outbreak. Samples were analyzed using RT-PCR. Of the 96 fecal samples, 46, 37, 29 and 7 goats remained positive for PPRV genome at 4, 8, 12 and 16 weeks after recovery, respectively. The results revealed the detection of PPRV genome in goats at 16 weeks after recovery. Therefore, it can be concluded that PPR disease has a carrier potential and goats recovered from PPR infection may act as source of carrier animals.

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“…Reverse transcriptase polymerase chain reaction (RT-PCR), a molecular diagnostic test based on amplification of the gene target offers a new strategy for diagnosis of PPRV, and is more sensitive than other tests. RT-PCR also offers the possibility of analysing the relationship between different PPRV strains for epidemiological studies (Shaila et al, 1996;Ozkul et al, 2002;Kwiatek et al, 2007;Wang et al, 2009;Balamurgan et al, 2010). Although RT-PCR overcomes the limitations of conventional tests, the sensitivity varies depending on the primer used and the gene targeted.…”

Section: Introductionmentioning

confidence: 99%

Comparison of genes as target for molecular diagnosis of peste des petits ruminants in goats

et al. 2013

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Peste des petits ruminants (PPR) are an acute viral disease of sheep and goats. Rapid and accurate diagnosis is essential for successful control. Reverse transcriptase polymerase chain reaction (RT-PCR), a molecular diagnostic test based on amplification of the gene target is more sensitive than other tests. The study was to find an efficient primer set and structural gene, which would be more specific and sensitive for detecting PPR virus (PPRV) in field samples. Six primer sets for six structural genes of PPR were used. Primer against NP gene (np3/np4) was specific and sensitive. To ensure efficient amplification and detection of viruses in field samples, more than one set of primers should be used and F and N gene specific primers were the most suitable. (Bangl. vet.

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Peste des Petits Ruminants (PPR) Outbreak in Tajikistan

Cited by 137 publications

References 28 publications

Phylogenetic analysis of peste des petits ruminants virus from outbreaks in Turkey during 2008–2012

Phylogenetic analysis of peste des petits ruminants virus from outbreaks in Turkey during 2008–2012

Detection of <em>Peste des Petits Ruminants</em> Virus genome in fecal material of goats after an outbreak in Punjab Province of Pakistan: A longitudinal study

Comparison of genes as target for molecular diagnosis of peste des petits ruminants in goats

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