Pest Control: Can Chitinases Help To Reduce Pesticide Use?

Silva, Ronivaldo Rodrigues da; Santos, Rafaela do Couto

doi:10.1021/acs.jafc.9b03219

Cited by 8 publications

(5 citation statements)

References 6 publications

(12 reference statements)

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“…Therefore, there is an urgent need to find other effective and friendly ways to solve such problems. Recently, biological control is one of the effective ways that have been widely studied [23,24]. Chitin exists mainly in the exoskeletal and gut linings of insects, so it is an effective target for pest control.…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Study of Chitin Metabolism and Detoxification-Related Genes in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) Based on Transcriptome Analysis

Shao

Zhang

et al. 2020

IJMS

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Glyphodes pyloalis Walker (Lepidoptera: Pyralididae) is a serious pest in the sericulture industry, which has caused damage and losses in recent years. With the widespread use of insecticides, the insecticide resistance of G. pyloalis has becomes increasingly apparent. In order to find other effective methods to control G. pyloalis, this study performed a transcriptome analysis of the midgut, integument, and whole larvae. Transcriptome data were annotated with KEGG and GO, and they have been shown to be of high quality by RT-qPCR. The different significant categories of differentially expressed genes between the midgut and the integument suggested that the transcriptome data could be used for next analysis. With the exception of Dda9 (GpCDA5), 19 genes were involved in chitin metabolism, most of which had close protein–protein interactions. Among them, the expression levels of 11 genes, including GpCHSA, GpCDA1, GpCDA2, GpCDA4, GPCHT1, GPCHT2a, GPCHT3a, GPCHT7, GpTre1, GpTre2, and GpRtv were higher in the integument than in the midgut, while the expression levels of the last eight genes, including GpCHSB, GpCDA5, GpCHT2b, GpCHT3b, GpCHT-h, GpPAGM, GpNAGK, and GpUAP, were higher in the midgut than in the integument. Moreover, 282 detoxification-related genes were identified and can be divided into 10 categories, including cytochrome P450, glutathione S-transferase, carboxylesterase, nicotinic acetylcholine receptor, aquaporin, chloride channel, methoprene-tolerant, serine protease inhibitor, sodium channel, and calcium channel. In order to further study the function of chitin metabolism-related genes, dsRNA injection knocked down the expression of GpCDA1 and GpCHT3a, resulting in the significant downregulation of its downstream genes. These results provide an overview of chitin metabolism and detoxification of G. pyloalis and lay the foundation for the effective control of this pest in the sericulture industry.

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Section: Discussionmentioning

confidence: 99%

Identification and Functional Study of Chitin Metabolism and Detoxification-Related Genes in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) Based on Transcriptome Analysis

Shao

Zhang

et al. 2020

IJMS

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“…An insect group h chitinase derived from the destructive agricultural pest Asian corn borer (Ostrinia furnacalis), Of Chi-h, is a critical chitinolytic enzyme as a result of its exclusive distribution in lepidopteran insects. , Of Chi-h possesses a long and narrow substrate-binding cleft with a catalytic center, which contains seven sugar subsites . This enzyme subsites are labeled as −5, −4, −3, −2, −1, +1, and +2, and the enzymatic cleavage happens between the −1 and +1 subsites.…”

Section: Introductionmentioning

confidence: 99%

Identification of Triazolo-quinazolinone Derivatives as Novel and Potent Chitinase OfChi-h Inhibitors Based on Structure-Based Virtual Screening

Dong,

Shen,

Jiang

et al. 2023

J. Agric. Food Chem.

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Insect chitinase, OfChi-h, from Ostrinia furnacalis, is considered as a promising target for the development of green pesticides. On the basis of the crystal structure of OfChi-h, we successfully obtained a triazolo-quinazolinone scaffold as the novel class of OfChi-h inhibitor via a structure-based virtual screening approach. Rational compound screening enabled us to acquire a potent OfChi-h inhibitor TQ19 with a K i value of 0.33 μM. Furthermore, the in vivo biological activity of target compounds was assayed. The results showed that compounds TQ8 and TQ19 could dramatically inhibit the growth and development of Ostrinia nubilalis larvae, and most of the compounds showed higher insecticidal activity than hexaflumuron. This present work reveals that triazolo-quinazolinone derivatives can serve as novel candidates for insect growth regulators.

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“…Chitin, poly(β-(1 → 4)- N -acetyl- d -glucosamine), is a protective but restrictive scaffold material of insect exoskeletons and internal chitinous structures. , In insects, glycoside hydrolase family 18 (GH18) chitinase (EC 3.2.1.14) cooperates with glycoside hydrolase family 20 (GH20) β- N -acetyl- d -hexosaminidase (Hex) (EC 3.2.1.52) to degrade chitin, which is tightly associated with the molting and pupation processes. , Chitinase-h is found exclusively in lepidopteran insects, and interference with the expression of chitinase-h leads to molting abnormalities and even death . Because of these features, chitinase-h has attracted interest as a promising target for pest control …”

Section: Introductionmentioning

confidence: 99%

“…6 Because of these features, chitinase-h has attracted interest as a promising target for pest control. 7 Of Chi-h is a processive chitinase-h from the destructive agricultural pest Ostrinia furnacalis. Its main function is to processively move along a single-carbohydrate chain and catalyze the hydrolysis of glycosidic bonds.…”

Section: ■ Introductionmentioning

confidence: 99%

Novel Inhibitors of an Insect Pest Chitinase: Design and Optimization of 9-O-Aromatic and Heterocyclic Esters of Berberine

Zhu

Chen

Shao

et al. 2021

J. Agric. Food Chem.

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OfChi-h, a lepidopteran-exclusive glycoside hydrolase family 18 (GH18) chitinase from the agricultural insect pest Ostrinia furnacalis, is a promising molecular target candidate for pest control and management. Berberine (BER), a traditional Chinese medicine, binds to a wide variety of glycosyl hydrolases via an identical mechanism, showing potential as a pesticide lead compound. In this work, we found that BER was a moderate inhibitor of OfChi-h with a Ki of 16.1 μM. To improve its efficacy, a series of BER derivatives featuring an ester bond linked to an aromatic or heterocyclic aromatic ring at the 9-position were designed and evaluated as effective OfChi-h inhibitors. The most potent compound, compound 19e with a nicotinate group, exhibited a Ki of 0.093 μM. Molecular docking analysis suggested that the common binding mode of BER derivatives featured a network of π–π stacking and electrostatic interactions and that the group at the 9-position enhanced the van der Waals and hydrogen bonding interactions. Administration of the BER derivative 19c to 4th-instar O. furnacalis larvae in an artificial diet led to their impaired growth and metamorphosis. This work provides a new starting point for the modification of BER for use in pest control.

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Pest Control: Can Chitinases Help To Reduce Pesticide Use?

Cited by 8 publications

References 6 publications

Identification and Functional Study of Chitin Metabolism and Detoxification-Related Genes in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) Based on Transcriptome Analysis

Identification and Functional Study of Chitin Metabolism and Detoxification-Related Genes in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) Based on Transcriptome Analysis

Identification of Triazolo-quinazolinone Derivatives as Novel and Potent Chitinase OfChi-h Inhibitors Based on Structure-Based Virtual Screening

Novel Inhibitors of an Insect Pest Chitinase: Design and Optimization of 9-O-Aromatic and Heterocyclic Esters of Berberine

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