Pesquisa De Anticorpos Anti-Leptospíricos Em Bubalinos / Leptospiral Antibodies in Buffaloes

Oliveira, G. C.; Silva, D. B.; Pinheiro, Vladimir; Langoni, Hélio

doi:10.15361/2175-0106.2013v29n4p33

Cited by 3 publications

(4 citation statements)

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“…In this region, serological studies have revealed the presence of anti-Leptospira spp. antibodies in buffaloes, with a prevalence ranging from 34.37 to 80.0% and predominance of the Sejroe serogroup (13)(14)(15), similar to what is observed in cattle. Nevertheless, there have been few studies on leptospirosis in buffaloes; thus, the aim of this study was to detect and perform the molecular characterization of Leptospira spp.…”

Section: Introductionsupporting

confidence: 73%

Identification of Pathogenic Leptospira Species in the Urogenital Tract of Water Buffaloes (Bubalus bubalis) From the Amazon River Delta Region, Brazil

et al. 2020

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In the current context of deforestation and fire in the Amazon, buffaloes could be a cost-effective and sustainable alternative for cattle production in the region, as they can convert low-quality foods and be raised in floodplain areas. However, little is known about the reproductive diseases that affect these animals; thus, the purpose of this study was to perform the molecular characterization of Leptospira spp. in the urogenital tract of water buffaloes (Bubalus bubalis) raised in the Amazon River Delta region in Brazil. Samples were collected from 114 kidneys, 204 ovaries, and 160 uterine swabs of slaughtered buffaloes in the Macapá microregion of Amapá State (Brazil) and were subjected to PCR to detect bacterial DNA. Positive amplicons were sequenced to identify Leptospira species. Among the total samples, 11/473 were PCR positive (2.3%), including 10 kidney samples and one uterine swab sample. DNA sequencing identified two pathogenic species from the kidney samples: L. interrogans, accounting for 60.0% (6/10) of these samples, and L. borgpetersenii, accounting for 20.0% (2/10), while 20.0% (2/10) were identified only at the genus level. The bacterium in the uterine swab sample was identified as L. interrogans with genetic proximity to strains belonging to the serovar Hardjo. This is the first report of leptospires species identified in buffaloes from the Amazon River Delta region and revealed that these animals may be carriers of different pathogenic Leptospira species, similar to bovines, including showing genital colonization.

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Section: Introductionsupporting

confidence: 73%

Identification of Pathogenic Leptospira Species in the Urogenital Tract of Water Buffaloes (Bubalus bubalis) From the Amazon River Delta Region, Brazil

et al. 2020

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“…Hardjo serovar was the third most frequent serovar between seropositive buffaloes in the state of Paraná, with 23.00% (49/213) seroreagent animals for this serovar. Some studies consider this the most frequent serovar found in bubaline herds in Brazil, with a prevalence of 43.4% (165/381) and 33.6% (51/152) in São Paulo (Langoni et al 1999, Favero et al 2002, 23,25% (20/86) and 76,44% (2901/3795) in Pará (Silva et al 2009, Dias et al 2014 in Pernambuco (Oliveira et al 2018), and 20.4% (18/88) in Marajó Island (Oliveira et al 2013). Thus, the prevalence of Hardjo serovar in bubaline from the state of Paraná can be considered lower than in other states and regions of Brazil.…”

Section: Discussionmentioning

confidence: 99%

“…Leptospirosis is a zoonosis caused by gram-negative bacteria of the genus Leptospira spp. belonging to the family Leptospiraceae, of the order Spirochetales (Adler & de la Peña 2010, Oliveira et al 2013, Pimenta et al 2019). The bacteria have several pathogenic serovars, whose distribution varies according to region, climate and susceptible hosts (Levett 2004, Balamurugan et al 2016.…”

Section: Introductionmentioning

confidence: 99%

“…Most leptospirosis cases in buffaloes are asymptomatic, characterized mainly by reproductive problems such as repeated oestrus and abortions in the final third of pregnancy (Galiero 2007, Marianelli et al 2007, Ijaz et al 2020. In acute cases, the disease buffaloes may present clinical signs such as lethargy, anorexia, fever, hemorrhages, jaundice, agalactia, mastitis, diarrhea, and abortions (Galiero 2007, Felt et al 2011, Oliveira et al 2013. Buffaloes can also be pathogen reservoirs since they may be asymptomatic and transmit the disease to humans and other mammals (Felt et al 2011, Denipitiya et al 2017.…”

Section: Introductionmentioning

confidence: 99%

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Serological frequency of Leptospira spp. in buffaloes (Bubalus bubalis) in Paraná state, Brazil

et al. 2023

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Leptospirosis is a zoonosis that can infect buffaloes and other mammalians, and it’s caused by Gram-negative bacteria of the genus Leptospira spp. The infection occurs through contact with urine or a contaminated environment with the mucous membranes of the animals, and the disease has two phases: leptospiremia and leptospiruria. The objective of this study was to determine the frequency of leptospirosis in buffaloes in the state of Paraná, Brazil, as well as the main serovars present in this area. A total of 276 blood samples were collected from buffaloes from 18 properties located in different cities and mesoregions of Paraná state. A microscopic agglutination test (MAT) was performed to detect the presence of anti-Leptospira spp. antibodies and to identify its serovars. The prevalence was 77.17% (213/276) of buffaloes seropositive for at least one serovar. The most frequent serovar was Icterohaemorrhagiae, followed by the Pomona serovar and serovar Hardjo. This study seems to be the first to determine the frequency of buffaloes with leptospirosis in the state of Paraná. Prevention and control measures are important to control the disease in herds.

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Prevalence of Leptospira serogroups in buffaloes from the Brazilian Amazon

Guedes

Souza

Oliveira

et al. 2020

Veterinary Medicine & Sci

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Although Brazil has one of the largest buffalo populations in the Americas, buffalo leptospirosis is still poorly explored when compared to that in bovines; thus, the aim of this research was to carry out a large serological study for leptospirosis in this species in the Brazilian Amazon. For this, we collected 1,405 serum samples from buffaloes raised in the Amazon delta region, which is considered a major area of buffalo production in Brazil. The test used was a microscopic agglutination test (MAT) adopting 34 Leptospira antigens, some of which have never been tested for buffaloes in Brazil, including autochthonous strains; in total, 20 serogroups were evaluated. From the total of 1,405 serum samples, 894 (63.6%) reacted in the MAT to at least one of the 20 serogroups, and 511 (36.4%) did not react. The serogroups Sejroe, Autumnalis and Pomona were the most prevalent, with titres ranging from 100 to 12,800, and the autochthonous strains used were not significant in relation to the reference serovars. Leptospirosis in buffaloes seems to have a serological profile similar to leptospirosis in cattle, mainly due to the prevalence of the Sejroe serogroup; however, the results of this study suggested that in the Brazilian Amazon, Leptospira strains that are serologically distinct from the autochthonous strains isolated in the southeastern region of Brazil may be circulating in these animals. Other serovars could also be inserted into the panel of antigens used in MAT for serological studies on buffaloes.

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Pesquisa De Anticorpos Anti-Leptospíricos Em Bubalinos / Leptospiral Antibodies in Buffaloes

Cited by 3 publications

References 0 publications

Identification of Pathogenic Leptospira Species in the Urogenital Tract of Water Buffaloes (Bubalus bubalis) From the Amazon River Delta Region, Brazil

Identification of Pathogenic Leptospira Species in the Urogenital Tract of Water Buffaloes (Bubalus bubalis) From the Amazon River Delta Region, Brazil

Serological frequency of Leptospira spp. in buffaloes (Bubalus bubalis) in Paraná state, Brazil

Prevalence of Leptospira serogroups in buffaloes from the Brazilian Amazon

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