Perusing biochemical antioxidant enzymes as selection criteria under drought stress in wheat varieties

Tabarzad, Amir; Ayoubi, Boshra; Riasat, Mehrnaz; Saed-Moucheshi, Armin; Pessarakli, Mohammad

doi:10.1080/01904167.2017.1346679

Cited by 20 publications

(16 citation statements)

References 20 publications

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“…Similarly, Riasat et al (2019) nominated SOD activity as the most appropriate trait to obtain high tolerant triticale genotypes. In addition, there are several studies on different crops that are in concordance with our results regarding the importance of biochemical features as the selection criteria (Riasat et al, 2020;Tabarzad et al, 2017;Vosough et al, 2019;Zahedi et al, 2016). Zahedi et al (2016) showed that SOD along with CAT and proline content were the effective biochemical traits toward the grain yield of barley genotypes and they introduced these traits as the candidate features capable of being used as indirect criteria in barely breeding.…”

Section: Discussionsupporting

confidence: 91%

“…magnesium-SOD (Mn-SOD), copper/zinc-SOD (Cu/Zn-SOD), and iron-SOD (Fe-SOD). Many studies have substantiated altered expression rate for each of the SOD isozymes under varies situation and stresses in different plants species (Alscher et al, 2002;Hasanuzzaman et al, 2020;Saed-Moucheshi et al, 2014b) and even different genotypes within a species (Elshafei, 2020;Tabarzad et al, 2017;Zahedi et al, 2016). Therefore, delving over the SOD expressional mechanisms that are active in its responsive pathways along with identifying related regulatory elements may provide new insights toward understanding and recognizing the resistance mechanisms in plants.…”

Section: Introductionmentioning

confidence: 99%

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Superoxide Dismutase (SOD): From Genomics and Expression Profiling to Heritability and Phenotypic Variability in Triticale Under Drought Stress

Saed-Moucheshi

Sohrabi

Fasihfar

et al. 2020

Preprint

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BackgroundThe main objectives of the current study were to find the possible structural association between the activity of enzymatic antioxidants and grain yield of triticale plants along with identify the genotypic variability of SOD activity among different triticale elite genotypes using novel statistical methods. Additionally, the difference between expression rate of SOD isozymes (Mn-SOD, Cu/Zn-SOD, and Fe-SOD) were tested to find out about the relationship between expression rate and the drought resistance of triticale selected genotypes. The final goal of this study was to introduce source code in SAS language to estimate the genetic parameters based on combined ANOVA model that could be applicable in all field studies.MethodsThirty genotypes of triticale were studied during six years in three different locations to model the relationship of enzymatic antioxidants and grain yield under drought stress condition. In every year, two separate field trials were used to assess the effect of normal irrigation (irrigation trial) and drought stress conditions (stress trials) by withholding irrigation at early heading stage. Accordingly, based on the results of genetic variability, heatmap analysis, biplot graph, and clustering, two genotypes with the highest genetic distance were selected to appraise the differential expression profiling of three SOD isozyme in shoot and root organs.ResultsField experiments and bioinformatics results showed that superoxide dismutase (SOD) was the most influential antioxidant in triticale tolerance to drought stress. Additionally, Mn-SOD showed high expression levels for both genotypes but with higher rates in tolerant genotype. Also, Mn-SOD expression rate in shoot was higher than root, but the expression level of Fe-SOD was conversely higher in the root. Additionally, Fe-SOD expression levels showed no significant difference in two used genotypes. The expression level of Cu/Zn-SOD was higher in root of the tolerant genotype while lower in root of susceptible genotype, as compared with their shoot.ConclusionHeatmap analysis that is applied for the first time in a screening program, showed to be highly capable of distinguishing elite genotypes and pointing out the proper features for selection criteria. Bioinformatics results indicated that SOD is more important than other enzymatic antioxidant for being considered as selection criteria or candidate gene for transgenic purposes. Based on expressional results, Mn-SOD announced as general isozyme that probably is highly expressed in most of the species, while, Cu/Zn-SOD as was introduced as a genotype specific isozyme that is likely more expressed in tolerant genotypes of triticale.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Superoxide Dismutase (SOD): From Genomics and Expression Profiling to Heritability and Phenotypic Variability in Triticale Under Drought Stress

Saed-Moucheshi

Sohrabi

Fasihfar

et al. 2020

Preprint

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“…Furthermore, LFW and LDW in the normal environment and SFW, SDW, RWC and SOD in the HTS environment had strong associations with TGW and yield, which support our hypothesis that seedling traits can be used as a selection parameter for heat tolerance. Previously, SOD and CAT were identified as useful indirect selection criteria for drought tolerance in wheat based on their strong positive correlations with grain yield (Afzal et al 2017; Tabarzad et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Agronomic, physiological and molecular characterization of rice mutants revealed key role of ROS and catalase in high temperature stress tolerance

Zafar

Hameed

Ashraf

et al. 2019

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Plants adapt to harsh environments particularly high temperature stress by regulating their physiological and biochemical processes, which are key tolerance mechanisms. Thus, identification of heat-tolerant rice genotypes and reliable selection indices are crucial for rice improvement programs. Here, we evaluated the response of a rice mutant population for high-temperature stress at the seedling and reproductive stages based on agronomic, physiological and molecular traits. The estimate of variance components revealed significant differences (P<0.001) among genotypes, treatments and their interaction for almost all traits. Principal component analysis showed significant diversity among the genotypes and traits under high-temperature stress. The mutant ‘HTT-121’ was identified as the most heat tolerant mutant with higher grain yield, panicle fertility, cell membrane thermo-stability (CMTS) and antioxidant enzyme levels under heat stress conditions. Various seedling-based morpho-physiological traits (leaf fresh weight, relative water contents, malondialdehyde, CMTS) and biochemical traits (superoxide dismutase, catalase and hydrogen peroxide) explained variations in grain yield that could be used as selection indices for heat tolerance in rice at early growth stages. Notably, heat sensitive mutants showed a significant accumulation of ROS level, reduced activities of catalase and upregulation of OsSRFP1 expression under heat stress, suggesting their key role in regulating heat tolerance in rice. The heat-tolerant mutants identified in this study could be used in breeding programs and the development of mapping populations to unravel the underlying genetic architecture for heat-stress adaptability.Summary text for table of contentsHeat stress probably due to changing climate scenario has become a serious threat for global rice production. On the other side, efforts to develop high yielding cultivars have led to the reduced genetic variability to withstand harsh environmental conditions. This study aimed to identify novel heat tolerant mutants developed through gamma irradiation which will provide a unique genetic resource for breeding programs. Further, we have identified reliable selection indices for screening heat-tolerant rice germplasm at early growth stages.

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“…It was observed that concentration of various antioxidant enzymes like peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) increased significantly to mediate the effects of ROS for mitigating the oxidative stress in wheat [34][35][36][37]. Hence, enzymatic antioxidants have important role in coping drought stress, and thus can be used in screening drought tolerant wheat varieties [10].…”

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confidence: 99%

EMS-based mutants are useful for enhancing drought tolerance in spring wheat

Zahra¹,

Zulfiqar

Hussain

et al. 2021

Preprint

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Sustainable wheat production in drought prone areas can be achieved by developing resilient wheat varieties. In the present study, chemical mutagenesis was used to induce mutations in a cultivated wheat variety ‘NN-Gandum-1’. In total, 44 mutants were selected based on their high yield potential for exposing to well-watered (W1) and rainfed (W2) conditions for one season. Then 24 mutants were selected, and were exposed to W1 and W2 regimes. On the basis of least relative reduction in physiological parameters under W2 regime, five mutants were selected for conducting exome capturing assays. In total, 184 SNPs were identified in nine genes (ABC transporter type 1, Aspartic peptidase, Cytochrome P450, transmembrane domain, Heavy metal-associated domain, HMA, NAC domain, NAD (P)-binding domain, S-type anion channel, Ubiquitin-conjugating enzyme E2 and UDP-glucuronosyl/UDP-glucosyltransferase). Maximum number of mutations were observed in chr.2D, which contained mutations in three genes i.e. ABC transporter type 1, NAD (P)-binding domain and UDP-glucuronosyl/UDP-glucosyltransferase which may have a role in conferring drought tolerance. The selected mutants were further tested for studying their biochemical responses under both the regimes for two years. The extent of membrane damage was estimated through malondialdehydeand hydrogen per oxidase and tolerance to drought stress was assessed via antioxidant enzymes in leaves. The selected mutants under drought stress increased the accumulation of proline content, total soluble sugars, total free amino acids, while decreased total chlorophyll content, carotenoids and total soluble protein. Finally, the procedure of narrowing down the number of developed mutants from a large mutation population (>4000) is found useful for exploring the complex trait like drought without compromising yield potential. These mutants can further be explored to understand the genetic circuits of drought tolerance in wheat which will pave the way towards improving livelihood of resource poor farming community mostly relying on cereal food.

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Perusing biochemical antioxidant enzymes as selection criteria under drought stress in wheat varieties

Cited by 20 publications

References 20 publications

Superoxide Dismutase (SOD): From Genomics and Expression Profiling to Heritability and Phenotypic Variability in Triticale Under Drought Stress

Superoxide Dismutase (SOD): From Genomics and Expression Profiling to Heritability and Phenotypic Variability in Triticale Under Drought Stress

Agronomic, physiological and molecular characterization of rice mutants revealed key role of ROS and catalase in high temperature stress tolerance

EMS-based mutants are useful for enhancing drought tolerance in spring wheat

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