Pertussis toxin selectively abolishes hormone induced lowering of cytosolic calcium in GH<sub>3</sub> cells

Schlegel, Werner; Wuarin, François; Zbaren, Claude; Wollheim, Claes B.; Zahnd, G

doi:10.1016/0014-5793(85)80835-8

Cited by 56 publications

(12 citation statements)

References 32 publications

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“…This effect was less marked, however, than the decrease following cell treatment with all three hormones together (not shown). As reported previously for the parent cell line GH, (32), VIP stimulation of a population of control GH,C, cells led to a small transient rise in the average [Ca' 'Ii. dependent on extracellular C a 2 + .…”

Section: Hormone Treartnent Decreases a C Activitysupporting

confidence: 78%

Spontaneous Intracellular Calcium Oscillations and G^sα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells

Chiavaroli

Cooper

Boyajian

et al. 1992

J Neuroendocrinology

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Cells of the pituitary tumour cell line GH(4) C(1) were exposed to epidermal growth factor, estradiol and insulin for 5 days, a treatment which resulted in 1) increased prolactin storage in secretory granules, 2) the loss of spontaneous [Ca(2+) ](1) oscillations, and 3) a selective reduction of the protein G(s) α, seen in immunoblots, cholera toxin labelling, and vasoactive intestinal peptide stimulation of adenylyl cyclase. In contrast, the glucocorticoid dexamethasone, which increases the expression of G(s) α, partially restored spontaneous [Ca(2+) ](1) oscillations and decreased prolactin storage. It is concluded that G(s) α levels in tumoral cells result in spontaneous electrical activity which may empty prolactin stores by the continuous activation of exocytosis.

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Section: Hormone Treartnent Decreases a C Activitysupporting

confidence: 78%

Spontaneous Intracellular Calcium Oscillations and G^sα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells

Chiavaroli

Cooper

Boyajian

et al. 1992

J Neuroendocrinology

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“…Thorner, Hackett, Murad & MacLeod, 1980; Ray & Wallis, 1982¿>; Merritt & Brown, 1984). Studies employing the calcium indicators Quin-2 and Fura-2 (Schofield, 1983;Koch et al 1985;Schlegel, Wuarin, Zbaren et al 1985;Hart, Ray & Wallis, 1986;Malgaroli, Vallar, Elahi et al 1987) and 45Ca2+ (Schofield & Bicknell, 1978;Schrey, Clark & Franks, 1986) have confirmed this idea. Whether this effect is associated with changes in the intracellular concentration of inositol trisphosphate is not clear and is the subject of some controversy (Brown, Baird, Quilliam et al 1985;Canónico, Jarvis, Judd & MacLeod, 1986;Enjalbert, Sladeczek, Guillon et al 1986;Journot, Hornberger, Pantaloni et al 1987).…”

Section: Introductionmentioning

confidence: 92%

Actions of pertussis toxin on the inhibitory effects of dopamine and somatostatin on prolactin and growth hormone release from ovine anterior pituitary cells

Boyd

Ray²,

Wallis³

1988

Journal of Molecular Endocrinology

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Forskolin and the phorbol ester 12-O-tetradecanoylphorbol 13-acetate stimulate prolactin and GH release from ovine anterior pituitary cells cultured in vitro. Dopamine and somatostatin inhibit release of prolactin and GH respectively, after stimulation by these agents, but without effects on intracellular cyclic AMP concentrations. In each case the inhibitory effects were reversed by pretreatment of cells with pertussis toxin, in a dose-related fashion (1-100 ng/ml), again without affecting cyclic AMP levels. The results suggest that the inhibitory effects of dopamine and somatostatin in this system are mediated by one or more pertussis toxin-sensitive G proteins, and that these act by a mechanism which does not involve inhibition of adenylate cyclase.

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“…Several lines of evidence implicate G ␣i in signaling by the SSTRs. With the exception of modulation of the Na ϩ /H ϩ antiporter (20), the effector-modulating activities of the SSTRs are sensitive to pertussis toxin-catalyzed ADP ribosylation, which inactivates G ␣i and G ␣o (27,45,57). Antibodies directed at the carboxy-terminal domains of G ␣i1-3 block formation of high-affinity binding sites in soluble brain SSTRs, in agreement with inhibition of the formation of an SSTR-G ␣i complex (35).…”

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confidence: 98%

Functional Coupling of a Mammalian Somatostatin Receptor to the Yeast Pheromone Response Pathway

Price¹,

Kajkowski²,

Hadcock³

et al. 1995

Molecular and Cellular Biology

127

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A detailed analysis of structural and functional aspects of G-protein-coupled receptors, as well as discovery of novel pharmacophores that exert their effects on members of this class of receptors, will be facilitated by development of a yeast-based bioassay. To that end, yeast strains that functionally express the rat somatostatin receptor subtype 2 (SSTR2) were constructed. High-affinity binding sites for somatostatin ([125I-Tyr-11]S-14) comparable to those in native tissues were detected in yeast membrane extracts at levels equivalent to the alpha-mating pheromone receptor (Ste2p). Somatostatin-dependent growth of strains modified by deletion of genes encoding components of the pheromone response pathway was detected through induction of a pheromone-responsive HIS3 reporter gene, enabling cells to grow on medium lacking histidine. Dose-dependent growth responses to S-14 and related SSTR2 subtype-selective agonists that were proportional to the affinity of the ligands for SSTR2 were observed. The growth response required SSTR2, G alpha proteins, and an intact signal transduction pathway. The sensitivity of the bioassay was affected by intracellular levels of the G alpha protein. A mutation in the SST2 gene, which confers supersensitivity to pheromone, was found to significantly enhance the growth response to S-14. In sst2 delta cells, SSTR2 functionally interacted with both a chimeric yeast/mammalian G alpha protein and the yeast G alpha protein, Gpa1p; to promote growth. These yeast strains should serve as a useful in vivo reconstitution system for examination of molecular interactions of the G-protein-coupled receptors and G proteins.

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Pertussis toxin selectively abolishes hormone induced lowering of cytosolic calcium in GH₃ cells

Cited by 56 publications

References 32 publications

Spontaneous Intracellular Calcium Oscillations and G^sα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells

Spontaneous Intracellular Calcium Oscillations and G^sα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells

Actions of pertussis toxin on the inhibitory effects of dopamine and somatostatin on prolactin and growth hormone release from ovine anterior pituitary cells

Functional Coupling of a Mammalian Somatostatin Receptor to the Yeast Pheromone Response Pathway

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Pertussis toxin selectively abolishes hormone induced lowering of cytosolic calcium in GH3 cells

Cited by 56 publications

References 32 publications

Spontaneous Intracellular Calcium Oscillations and Gsα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells

Spontaneous Intracellular Calcium Oscillations and Gsα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells

Actions of pertussis toxin on the inhibitory effects of dopamine and somatostatin on prolactin and growth hormone release from ovine anterior pituitary cells

Functional Coupling of a Mammalian Somatostatin Receptor to the Yeast Pheromone Response Pathway

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Pertussis toxin selectively abolishes hormone induced lowering of cytosolic calcium in GH₃ cells

Spontaneous Intracellular Calcium Oscillations and G^sα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells

Spontaneous Intracellular Calcium Oscillations and G^sα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells