Perturbed angular correlation studies of 111In-labelled platelets

Methodologic and Basic Aspects of Indium-111 Platelets

“…The rather striking discrepancies between the chromatographic findings of Hudson et al 17 on the one hand, and those of Mathias and Welch 24 and Joist et al, 20 on the other hand, are likely to be due to technical differences in homogenation procedure and/or chromatography (buffer systems), since the association of 111 In with platelet components is relatively weak and highly dependent on ion and protein composition of the medium. This makes it likely that transfer of 111 In among cytosol proteins occurs following platelet disruption, a concept supported also by the results of perturbed angular correlation studies carried out by Pandian et al 32 In attempts to define further the nature of the platelet components that bind 111 In, Triton X-100 treated platelet cytosol and particulate fraction from 111 In platelets were subjected to crossed Immunoelectrophoresis using antiserum against solubilized whole human platelets prepared in rabbits. Panel A in Figure 4 shows the profile obtained with cytosol after protein staining with Coomassie blue and panel B, the autoradiograph of the same plate.…”

Perturbed angular correlation spectroscopy and its application to metal sites in proteins: possibilities and limitations

“…Another biological application of the integral PAC technique is measurements on 111 In-labelled human platelets (Pandian, Mathias & Welch, 1982, and references therein), demonstrating the binding of l u I n to large macromolecules within the cells.…”