Perturbation of formate pathway and NADH pathway acting on the biohydrogen production

Liu, Dong; Sun, Yunze; Li, Yuhao; Lu, Yuan

doi:10.1038/s41598-017-10191-7

Cited by 32 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From a holistic point of view, all the steps involved in BioH 2 generation metabolism could be targeted as steps to enhance BioH 2 generation (HY and HER). Two major metabolic pathways, namely, butyrate and acetate, are mainly associated with the activities of hydrogenase and the generation of H 2 during dark fermentation [93][94][95]. From a stoichiometric perspective, the metabolic route towards acetate generates two times that of butyrate pathways [96,97].…”

Section: Impact Of Ion Additionmentioning

confidence: 99%

A Review of Enhancement of Biohydrogen Productions by Chemical Addition Using a Supervised Machine Learning Method

Liu

et al. 2021

Energies

View full text Add to dashboard Cite

In this work, the impact of chemical additions, especially nano-particles (NPs), was quantitatively analyzed using our constructed artificial neural networks (ANNs)-response surface methodology (RSM) algorithm. Fe-based and Ni-based NPs and ions, including Mg2+, Cu2+, Na+, NH4+, and K+, behave differently towards the response of hydrogen yield (HY) and hydrogen evolution rate (HER). Manipulating the size and concentration of NPs was found to be effective in enhancing the HY for Fe-based NPs and ions, but not for Ni-based NPs and ions. An optimal range of particle size (86–120 nm) and Ni-ion/NP concentration (81–120 mg L−1) existed for HER. Meanwhile, the manipulation of the size and concentration of NPs was found to be ineffective for both iron and nickel for the improvement of HER. In fact, the variation in size of NPs for the enhancement of HY and HER demonstrated an appreciable difference. The smaller (less than 42 nm) NPs were found to definitely improve the HY, whereas for the HER, the relatively bigger size of NPs (40–50 nm) seemed to significantly increase the H2 evolution rate. It was also found that the variations in the concentration of the investigated ions only statistically influenced the HER, not the HY. The level of response (the enhanced HER) towards inputs was underpinned and the order of significance towards HER was identified as the following: Na+ > Mg2+ > Cu2+ > NH4+ > K+.

show abstract

Section: Impact Of Ion Additionmentioning

confidence: 99%

A Review of Enhancement of Biohydrogen Productions by Chemical Addition Using a Supervised Machine Learning Method

Liu

et al. 2021

Energies

View full text Add to dashboard Cite

show abstract

“…In terms of economy and environmental protection, they are not suitable for long-term industrial hydrogen production [5]. Compared with traditional thermochemical hydrogen production technology, biotechnological hydrogen fermentation is a more promising hydrogen production process, which does not require harsh conditions and requires only inexpensive equipment [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…During dark fermentation for hydrogen production, E. aerogenes produces hydrogen through the formate pathway and the NADH pathway [7,10]. In the formate pathway, pyruvate is converted by pyruvate formate lyase (p ) to produce acetyl coenzyme A and formic acid.…”

Section: Introductionmentioning

confidence: 99%

Optimization of hydrogen production in Enterobacter aerogenes by Complex Ⅰ peripheral fragments destruction and maeA overexpression

Jiang

Bai

Gao

et al. 2023

Preprint

View full text Add to dashboard Cite

As a concentrated energy source with high added value, hydrogen has great development prospects, with special emphasis on sustainable microbial production as a replacement for traditional fossil fuels. In this study, λ-Red recombination was used to alter the activity of Complex I by single and combined knockout of nuoE, nuoF and nuoG. In addition, the conversion of malic to pyruvic acid was promoted by overexpressing the maeA gene, which could increase the content of NADH and formic acid in the bacterial cells. Compared to the original strain, hydrogen production was 65% higher in the optimized strain IAM1183-EFG/M, in which the flux of the formic acid pathway was increased by 257%, the flux of the NADH pathway was increased by 13%, and the content of metabolites also changed significantly. In further bioreactor, scale-up IAM1183-EFG/M also showed strong industrial application potential, with a total hydrogen production of 4.76 L after 44h of fermentation, which significantly increased by 18% compared with the starting strain. This study provides a new direction for future exploration of microbial hydrogen production by combinatorial modification of multiple genes.

show abstract

“…In terms of economy and environmental protection, they are not suitable for long-term industrial hydrogen production [ 5 ]. Compared with traditional thermochemical hydrogen production technology, biotechnological hydrogen fermentation is a more promising hydrogen production process, which does not require harsh conditions and requires only inexpensive equipment [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…During dark fermentation for hydrogen production, E. aerogenes produces hydrogen through the formate pathway and the NADH pathway [ 7 , 10 ]. In the formate pathway, pyruvate is converted by pyruvate formate lyase ( pfl ) to produce acetyl coenzyme A and formic acid.…”

Section: Introductionmentioning

confidence: 99%

Optimization of hydrogen production in Enterobacter aerogenes by Complex I peripheral fragments destruction and maeA overexpression

et al. 2023

View full text Add to dashboard Cite

As a concentrated energy source with high added value, hydrogen has great development prospects, with special emphasis on sustainable microbial production as a replacement for traditional fossil fuels. In this study, λ-Red recombination was used to alter the activity of Complex I by single and combined knockout of nuoE, nuoF and nuoG. In addition, the conversion of malic to pyruvic acid was promoted by overexpressing the maeA gene, which could increase the content of NADH and formic acid in the bacterial cells. Compared to the original strain, hydrogen production was 65% higher in the optimized strain IAM1183-EFG/M, in which the flux of the formic acid pathway was increased by 257%, the flux of the NADH pathway was increased by 13%, and the content of metabolites also changed significantly. In further bioreactor, the total hydrogen production of the scale-up IAM1183-EFG/M after 44 h of fermentation was 4.76 L, which increased by 18% compared with the starting strain. This study provides a new direction for future exploration of microbial hydrogen production by combinatorial modification of multiple genes. Graphical Abstract

show abstract

Perturbation of formate pathway and NADH pathway acting on the biohydrogen production

Cited by 32 publications

References 32 publications

A Review of Enhancement of Biohydrogen Productions by Chemical Addition Using a Supervised Machine Learning Method

A Review of Enhancement of Biohydrogen Productions by Chemical Addition Using a Supervised Machine Learning Method

Optimization of hydrogen production in Enterobacter aerogenes by Complex Ⅰ peripheral fragments destruction and maeA overexpression

Optimization of hydrogen production in Enterobacter aerogenes by Complex I peripheral fragments destruction and maeA overexpression

Contact Info

Product

Resources

About