Perturbation of Fgf10 signal pathway in mouse embryonic palate by dexamethasone and vitamin B12 in vivo

Wang, He; Meng, Titan; Wu, Min; Shi, Bing; Lu, Shengjun; Li, Chenghao

doi:10.1016/j.jpedsurg.2010.05.018

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…While the exact mechanism was still unclear, the findings of some cohorts indicated that use of medication was observed to combine with destructive genes that result in NSCL/P. For instance, phenytoin combines Satb2 and Hoxa2 ( 25 ), and dexamethasone interacts with the Fgf10 signal pathway ( 26 ), which may increase the risk of NSCL/P development.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Environmental Exposures for Nonsyndromic Cleft Lip and/or Palate: A Case-Control Study

Sakran¹,

Abotaleb²,

Al-Rokhami³

et al. 2022

ijph

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Background: Orofacial cleft is among the most common developmental malformations in humans. This study aimed to identify the relationship between environmental factors and nonsyndromic cleft lip and/or palate (NSCL/P) in Northwest China. Methods: This case-control study was conducted in Gansu Province, China over two years (Jan. 1, 2017–Jan. 1, 2019). Overall, 600 NSCL/P cases and 660 normal control cases were finally enrolled in the current study. Data were collected by conducting face-to-face interviews with both parents of each case. Results: Univariate (χ2) analysis revealed 22 factors as being significantly associated with NSCL/P. Multivariate (stepwise logistic regression) analysis identified that 14 factors had statistically significant association with NSCL/P. Male gender (OR=0.789), paternal age at childbirth of 25-29 yr (OR=0.690), and folic acid supplement (OR=0.197) were found to be protective factors against NSCL/P. On the other hand, blood A-type, multiple births, positive family history of NSCLP (OR=6.660), parental consanguinity (OR=6.107), positive abortion history, high or low maternal childbearing age, and maternal passive smoking (OR=4.349), malnutrition (OR=4.431), infections, and drug use (OR=2.188) during early gestation were significant risk factors for NSCL/P. Conclusion: Parental age at childbirth of 25–29 yr, and folic acid supplement can reduce the risk of NSCL/P. By contrast, maternal passive smoking, infections, and drug use during early gestation period, and multiple births, parental consanguinity, positive family history, and maternal abortion history can increase the risk of NSCL/P. Identification of risk factors is essential in minimizing the incidence of NSCL/P in a particular population.

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Section: Discussionmentioning

confidence: 99%

Analysis of Environmental Exposures for Nonsyndromic Cleft Lip and/or Palate: A Case-Control Study

Sakran¹,

Abotaleb²,

Al-Rokhami³

et al. 2022

ijph

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“…An in vitro study on cultured murine palatal cells found that dexamethasone induced the differentiation of MEE cells into squamous epithelial cells, perturbing development of the palate epithelium (Huang, Shi, Sun, & Wang, 2005). An in vivo study found that dexamethasone exposure decreased expression of Fgf10 in the mesenchyme as well as Fgfr2b and sonic hedgehog ( Shh ) expression in the epithelium, thereby perturbing Fgf‐Hedgehog signaling axis (He et al, 2010). Interestingly, this study found that co‐exposure with vitamin B 12 restored gene expression and mesenchymal proliferation.…”

Section: Environmental Signaling Pathways In Orofacial Cleftsmentioning

confidence: 99%

Environmental mechanisms of orofacial clefts

Garland

Reynolds

Zhou

2020

Birth Defects Research

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Orofacial clefts (OFCs) are among the most common birth defects and impart a significant burden on afflicted individuals and their families. It is increasingly understood that many nonsyndromic OFCs are a consequence of extrinsic factors, genetic susceptibilities, and interactions of the two. Therefore, understanding the environmental mechanisms of OFCs is important in the prevention of future cases. This review examines the molecular mechanisms associated with environmental factors that either protect against or increase the risk of OFCs. We focus on essential metabolic pathways, environmental signaling mechanisms, detoxification pathways, behavioral risk factors, and biological hazards that may disrupt orofacial development.

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“…Recently, Cbl was shown to support palate fusion in the presence of concentrations of dexamethasone, which normally impaired fusion of murine palates in organ culture [119]. In vivo , Cbl in the presence of dexamethasone restored proliferation of the mesenchymal cells of the palate via increased expression of a growth factor—Fgf10—which is normally involved in craniofacial development [120], and thus, suggesting that Cbl may indirectly regulate the levels of growth factors essential for normal organogenesis.…”

Section: Mouse Modelsmentioning

confidence: 99%

Vitamin B12 Metabolism during Pregnancy and in Embryonic Mouse Models

2013

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Vitamin B12 (cobalamin, Cbl) is required for cellular metabolism. It is an essential coenzyme in mammals for two reactions: the conversion of homocysteine to methionine by the enzyme methionine synthase and the conversion of methylmalonyl-CoA to succinyl-CoA by the enzyme methylmalonyl-CoA mutase. Symptoms of Cbl deficiency are hematological, neurological and cognitive, including megaloblastic anaemia, tingling and numbness of the extremities, gait abnormalities, visual disturbances, memory loss and dementia. During pregnancy Cbl is essential, presumably because of its role in DNA synthesis and methionine synthesis; however, there are conflicting studies regarding an association between early pregnancy loss and Cbl deficiency. We here review the literature about the requirement for Cbl during pregnancy, and summarized what is known of the expression pattern and function of genes required for Cbl metabolism in embryonic mouse models.

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Perturbation of Fgf10 signal pathway in mouse embryonic palate by dexamethasone and vitamin B12 in vivo

Cited by 10 publications

References 16 publications

Analysis of Environmental Exposures for Nonsyndromic Cleft Lip and/or Palate: A Case-Control Study

Analysis of Environmental Exposures for Nonsyndromic Cleft Lip and/or Palate: A Case-Control Study

Environmental mechanisms of orofacial clefts

Vitamin B12 Metabolism during Pregnancy and in Embryonic Mouse Models

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