Animal tissue is material studied in class XI with coverage of epithelial tissue, connective tissue, muscle tissue and nerve tissue. Animal tissue material has characteristics that focus on visualizing animal tissue because tissue is an abstract material and to make it more real, it requires media in the form of emodules to visualize animal tissue material clearly. The purpose of this study was to determine the motivational response of students to the use of animal tissue e-modules. This type of research is descriptive research with a quantitative approach. The instrument used is a questionnaire with aspects assessed, namely the feasibility of content, language, benefits and graphics. Based on the results of the study, it was found that the response of students' learning motivation to the e-module was very good, namely with an average score of 77.14%, which means that students' learning motivation increased after using the animal tissue e-module.