Pertraction-adsorption in situ product removal system: Intensification of 2-phenyelthanol bioproduction

Červeňanský, Ivan; Mihaľ, Mário; Markoš, Jozef

doi:10.1016/j.seppur.2020.117283

Cited by 9 publications

(8 citation statements)

References 40 publications

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“…A regular offline cleaning (once every 3-5 weeks) for the external membrane module by flushing with water to remove the accumulated foulants was performed in previous studies (Xu et al, 2015;Xu et al, 2021), while a continuous high extraction rate was achieved in this work at least for 248 days by regularly recirculating without any offline washing or application of anti-fouling chemical agents. The broth biomass concentration in this work (Supplementary Figure 4) was relatively lower compared to most previous studies (Ge et al, 2015;Xu et al, 2018), and it has been reported that increase in biomass could result in an increase in conversion rate and concentration of products (Xu et al, 2018;Červeňanský et al, 2020), thus, further leading to a high extraction rate (Kucek et al, 2016a;Carvajal-Arroyo et al, 2020). Therefore, increasing the biomass concentration (e.g., adding carriers) would result in further increases in the MCCA extraction rate using internal membrane, albeit probably negatively affecting membrane fouling.…”

Section: The Effect Of Anti-membrane Fouling Strategies On Medium-chain Carboxylic Acid Production Rate and Extraction Rate By Internal Hmentioning

confidence: 53%

Long-Term Continuous Extraction of Medium-Chain Carboxylates by Pertraction With Submerged Hollow-Fiber Membranes

Bian

Angenent

et al. 2021

Front. Bioeng. Biotechnol.

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Medium-chain carboxylic acids (MCCAs), which can be generated from organic waste and agro-industrial side streams through microbial chain elongation, are valuable chemicals with numerous industrial applications. Membrane-based liquid-liquid extraction (pertraction) as a downstream separation process to extract MCCAs has been applied successfully. Here, a novel pertraction system with submerged hollow-fiber membranes in the fermentation bioreactor was applied to increase the MCCA extraction rate and reduce the footprint. The highest average surface-corrected MCCA extraction rate of 655.2 ± 86.4 mmol C m−2 d−1 was obtained, which was higher than any other previous reports, albeit the relatively small surface area removed only 11.6% of the introduced carbon via pertraction. This submerged extraction system was able to continuously extract MCCAs with a high extraction rate for more than 8 months. The average extraction rate of MCCA by internal membrane was 3.0- to 4.7-fold higher than the external pertraction (traditional pertraction) in the same bioreactor. A broth upflow velocity of 7.6 m h−1 was more efficient to extract MCCAs when compared to periodic biogas recirculation operation as a means to prevent membrane fouling. An even higher broth upflow velocity of 40.5 m h−1 resulted in a significant increase in methane production, losing more than 30% of carbon conversion to methane due to a loss of H2, and a subsequent drop in the H2 partial pressure. This resulted in the shift from a microbial community with chain elongators as the key functional group to methanogens, because the drop in H2 partial pressure led to thermodynamic conditions that oxidizes ethanol and carboxylic acids to acetate and H2 with methanogens as the syntrophic partner. Thus, operators of chain elongating systems should monitor the H2 partial pressure when changes in operating conditions are made.

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Section: The Effect Of Anti-membrane Fouling Strategies On Medium-chain Carboxylic Acid Production Rate and Extraction Rate By Internal Hmentioning

confidence: 53%

Long-Term Continuous Extraction of Medium-Chain Carboxylates by Pertraction With Submerged Hollow-Fiber Membranes

Bian

Angenent

et al. 2021

Front. Bioeng. Biotechnol.

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“…Even though a considerable increase in the production of 2-PE has been achieved through the aforementioned strategy, this concentration still leaves room for improvement when compared to the concentrations reported for other genetically modified microorganisms and using other operating strategies. 11,[32][33][34]…”

Section: Discussionmentioning

confidence: 99%

“…The step‐wise fed‐batch mode with L‐Phe presents advantages in terms of precursor inhibition and consumption 30,31 . Nevertheless, and even though increases in the production of 2‐PE have been achieved through the aforementioned strategy, there is still room for improvement 11,32‐34 …”

Section: Introductionmentioning

confidence: 99%

“…30,31 Nevertheless, and even though increases in the production of 2-PE have been achieved through the aforementioned strategy, there is still room for improvement. 11,[32][33][34] The main goal of this work was the study of 2-PE production from L-Phe biotransformation, using cheap feedstock as carbon source and Yarrowia as cell factory. Thus, the work addresses strains selection, culture conditions optimization, and scale-up of the process to bioreactor level.…”

Section: Introductionmentioning

confidence: 99%

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Valorization of crude glycerol as carbon source for the bioconversion of L‐phenylamine to 2‐phenylethanol by Yarrowia species

Braga

Freitas

Cordeiro

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND 2‐Phenylethanol (2‐PE) is an aromatic alcohol with a delicate fragrance of rose petals that is widely used in perfumes, cosmetics, foods, beverages, and pharmaceutical industries. The yeast Yarrowia can produce 2‐PE through L‐Phenylalanine (L‐Phe) biotransformation. However, it has not been properly explored in this field, despite its interesting characteristics for this process, such as the Crabtree negative trait. RESULTS This study explored the potential of different Yarrowia species (Y. lipolytica W29, CH 1/5, and CH 3/4, as well as Y. divulgata M 445/4) for 2‐PE production. It was observed that all strains were inhibited by 2‐PE concentrations above 2 g L−1, but only Y. lipolytica W29 was inhibited by 15 g L−1 L‐Phe. It was also confirmed that all strains were able to produce 2‐PE, with concentrations ranging from 1 to 2.2 g L−1. Afterwards, crude glycerol was used as an alternative carbon source for 2‐PE production. All strains grew and produced 2‐PE on this carbon source, but CH 1/5 strain was selected for further studies because it allowed the highest 2‐PE throughput. The production of 2‐PE with CH 1/5 was further studied in bioreactor batch cultures, where a low accumulation of 2‐PE was observed. However, a step‐wise fed‐batch culture allowed an increase in the production of 2‐PE to 3.2 g L−1. CONCLUSION To the best of the authors' knowledge, this value corresponds to the highest titer of 2‐PE obtained to date with Y. lipolytica. In this study, Y. lipolytica CH 1/5 is, for the first time, described as a good natural producer for 2‐PE. © 2021 Society of Chemical Industry (SCI).

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“…Mass transfer occurs by diffusion at pores mouth which allows a high interfacial area [34]. The use of HFMCs as an interface in a LLE process to reduce the toxicity of solvents and inhibition of fermentation products towards microorganisms has been studied by several authors [35][36][37][38][39]. The work of Jin and Yang in 1998 is a particularly successful and encouraging example, they presented an extractive fermentation process using an amine/oleyl alcohol extractant and a hollow-fiber membrane contactor to selectively remove propionic acid from the fermentation broth.…”

Section: Introductionmentioning

confidence: 99%

In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor

Combes

Imatoukene

Moussa

et al. 2022

Separation and Purification Technology

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Pertraction-adsorption in situ product removal system: Intensification of 2-phenyelthanol bioproduction

Cited by 9 publications

References 40 publications

Long-Term Continuous Extraction of Medium-Chain Carboxylates by Pertraction With Submerged Hollow-Fiber Membranes

Long-Term Continuous Extraction of Medium-Chain Carboxylates by Pertraction With Submerged Hollow-Fiber Membranes

Valorization of crude glycerol as carbon source for the bioconversion of L‐phenylamine to 2‐phenylethanol by Yarrowia species

In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor

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