Pertinence of Apoptosis Markers for the Improvement of In Vitro Fertilization (IVF)

Haouzi, D.; Hamamah, S.

doi:10.2174/092986709788186075

Cited by 39 publications

(29 citation statements)

References 130 publications

(161 reference statements)

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“…It is expected that CCs show signs of apoptosis at the final maturation stage. Some authors have even suggested to use these apoptotic genes during folliculogenesis as potential markers of oocyte development potential [52]. Surprisingly and in parallel to these apoptotic events, the expression of some cell death genes in CCs was markedly reduced.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Assidi

Montag

Ven

et al. 2010

J Assist Reprod Genet

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Purpose To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria. Methods Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intrapatient analysis were performed on 17 selected candidates. Results Seven genes were differentially (p≤0.05) associated to successful pregnancy and implantation. These biomarkers could be used to predict the oocyte developmental competence. Conclusions These genomic markers are a powerful reinforcement of morphological approaches of oocyte selection.Their large-scale validation could increase pregnancy outcome and single embryo transfer efficiency.

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Section: Discussionmentioning

confidence: 99%

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Assidi

Montag

Ven

et al. 2010

J Assist Reprod Genet

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“…During postnatal life, the oocyte stockpile continues to be depleted until exhaustion at the moment of menopause (for reviews, see and Haouzi & Hamamah (2009)). Moreover, if the few ovulated oocytes (300-400 during a woman's reproductive life) are not fertilized on time, they will succumb to apoptotic death .…”

Section: Introductionmentioning

confidence: 99%

Involvement of BCL2 family members in the regulation of human oocyte and early embryo survival and death: gene expression and beyond

Boumela¹,

Assou²,

Aouacheria³

et al. 2011

Reproduction

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In women, up to 99.9% of the oocyte stockpile formed during fetal life is decimated by apoptosis. Apoptotic features are also detected in human preimplantation embryos both in vivo and in vitro. Despite the important consequences of cell death processes to oocyte competence and early embryonic development, little is known about its genetic and molecular control. B cell lymphoma-2 (BCL2) family proteins are major regulators of cell death and survival. Here, we present a literature review on BCL2 family expression and protein distribution in human and animal oocytes and early embryos. Most of the studies focused on the expression of two antagonistic members: the founding and survival family member BCL2 and its proapoptotic homolog BAX. However, recent transcriptomic analyses have identified novel candidate genes related to oocyte and/or early embryonic viability (such as BCL2L10) or commitment to apoptosis (e.g. BIK). Interestingly, some BCL2 proteins appear to be differentially distributed at the subcellular level during oocyte maturation and early embryonic development, a process probably linked to the functional compartmentalization of the ooplasm and blastomere. Assessment of BCL2 family involvement in regulating the survival of human oocytes and embryos may be of particular value for diagnosis and assisted reproductive technology. We suggest that implications of not only aberrant gene expression but also abnormal subcellular protein redistribution should be established in pathological conditions resulting in infertility.

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“…Apoptosis occurs frequently early embryonic development and has a significant impact on the development of the embryo [39]. Therefore, we investigated the expression patterns of three apoptosis-related genes, Bcl-xl, Bak and Caspase-3.…”

Section: Discussionmentioning

confidence: 99%

Scriptaid affects histone acetylation and the expression of development-related genes at different stages of porcine somatic cell nuclear transfer embryo during early development

et al. 2013

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Although the somatic cell nuclear transfer (SCNT) technique has been used extensively for cloning and generating transgenic pigs, the cloning efficiency is still very low. It has been proposed that the low efficiency of this technique is the result of incomplete epigenetic reprogramming and abnormal gene expression during early embryonic development. In this study, we investigate the effect of Scriptaid, a low-toxicity histone deacetylase inhibitor, on the developmental competence of porcine SCNT embryos. We found that treating SCNT embryos with 500 nmol/L Scriptaid for 15 h after activation significantly enhanced the blastocyst formation rate (27.7%) compared with the untreated group (control) (12.2%, P<0.05). Using an immunofluorescence technique to measure the average fluorescence intensity, we also found that treating SCNT embryos with Scriptaid increased the level of histone acetylation on histone H3 at lysine 14 (acH3K14). Furthermore, treating embryos with Scriptaid increased the expression level of three genes that play important roles during embryonic development (Oct4, Klf4 at the blastocyst stage and Nanog at the 4-cell stage). Moreover, the expression level of the apoptosis-related gene Caspase-3 was significantly lower in the Scriptaid-treated SCNT embryos compared with the control SCNT embryos at the 4-cell and blastocyst stages. In conclusion, these results indicate that Scriptaid treatment improves the development and nuclear reprogramming of porcine SCNT embryos.

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Pertinence of Apoptosis Markers for the Improvement of In Vitro Fertilization (IVF)

Cited by 39 publications

References 130 publications

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study

Involvement of BCL2 family members in the regulation of human oocyte and early embryo survival and death: gene expression and beyond

Scriptaid affects histone acetylation and the expression of development-related genes at different stages of porcine somatic cell nuclear transfer embryo during early development

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