Perthes Disease: Immunological Aspects

Abstract

Considering a stage of the pathological process patients (boys at the age of 8-12 years) were divided into two (2) groups. Group I included 14 patients with the fragmentation stage (Perthes disease Stage II). Group 2 included 15 children with Perthes disease Stage III (the stage of re-ossification). Perthes disease regardless of the stage of the disease was characterized by the increase in oxygen-dependent and lysosomal phagocytic activity of neutrophils, the increase in the number of early extracellular traps, as well as by increased concentrations of pro-inflammatory cytokines (IL-1β and TNFa), IgE, decreased concentrations of IL-18. The fragmentation stage was characterized by moderate activation of cellular immunity with a prevailing increase in the number of T-lymphocytes with early activation markers (CD25). At the re-ossification stage the predominance of T-lymphocytes was observed with late activation markers (HLADR), being accompanied by moderate activation of humoral immunity (increased concentrations of class A and G serum immunoglobulins). The obtained data can be used as additional criteria for clarifying Perthes disease stage, predicting osteonecrosis development when making decision of the feasibility of performing reconstructive surgeries on the joint.

Construction of ceRNA network based on RNA-seq for identifying prognostic lncRNA biomarkers in Perthes disease

“…For instance, VEGF plays an important role in promoting the growth and repair of blood vessels that may be disrupted in individuals with Perthes disease (Kim et al, 2004). Additionally, the occurrence of inflammation is fundamental to the pathogenesis of Perthes disease, and previous studies have reported that genetic variations in certain inflammation-related genes, including IL-6, TLR4, TNFA, and IL-3, are associated with the development of this condition (Srzentić et al, 2014;Teplen'kiy et al, 2020).…”