Pertactin-deficient Bordetella pertussis isolates in Poland—a country with whole-cell pertussis primary vaccination

Polak, Maciej; Zasada, Aleksandra Anna; Mosiej, Ewa; Krysztopa-Grzybowska, Katarzyna; Witkowski, Lucjan; Rzeczkowska, Magdalena; Piekarska, Katarzyna; Lutyńska, Anna

doi:10.1016/j.micinf.2018.12.001

Cited by 19 publications

(13 citation statements)

References 43 publications

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“…All 170 isolates contained sequences encoding the same protein as the tcfA2 allele, which is reactive with the pertussis LFIA. Broadening our geographic focus, we reviewed 784 other B. pertussis isolates for which TcfA sequence information was available 34,[36][37][38][39][40] . Only 19 of these isolates were from the U.S. 34,39 .…”

Section: Resultsmentioning

confidence: 99%

“…Only 19 of these isolates were from the U.S. 34,39 . The remaining 765 were from Poland, the Netherlands, China, the United Kingdom, Finland, Sweden, France, Germany, Japan, Italy, and Belgium (listed in descending order based on the number of isolates sourced from each country) 34,[36][37][38][39][40] . These isolates were collected between 1949 and 2017 34,[36][37][38][39][40] .…”

Section: Resultsmentioning

confidence: 99%

“…The LFIA's inclusivity was further analyzed in silico with sequences from 954 B. pertussis isolates from 12 countries [34][35][36][37][38][39][40] . In sum, of 954 B. pertussis isolates (including 10 tcfA deletion and frameshift mutants identified through a dedicated search for isolates with deficient TcfA expression 39 ), we would predict that the pertussis LFIA would be highly inclusive and would react with 98% of them (i.e.…”

Section: Discussionmentioning

confidence: 99%

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Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis

Burnham-Marusich

Olsen

Scarbrough

et al. 2020

Sci Rep

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Pertussis is a highly contagious disease for which prompt, point-of-care diagnosis remains an unmet clinical need. Results from conventional test modalities (nucleic acid detection, serology, and culture) take hours to days. To overcome this challenge, we identified a new biomarker (tracheal colonization factor A, TcfA) for detection of Bordetella pertussis infection by lateral flow immunoassay (LFIA). We developed a library of 28 epitope-mapped monoclonal antibodies against TcfA and incorporated three antibodies into a LFIA. The LFIA did not cross-react with common bacterial or fungal organisms, but did react with nine distinct B. pertussis strains. The minimal linear epitope sequences targeted by the LFIA were conserved in 98% of 954 B. pertussis isolates collected across 12 countries from 1949–2017. The LFIA’s limit of detection was 3.0 × 105 CFU/mL with B. pertussis cells in buffer, 6.2 × 105 CFU/mL with nasopharyngeal washes from a non-human primate model, and 2.3 ng/mL with recombinant TcfA. The LFIA reacted with patient nasopharyngeal swab specimens containing as few as 1.8 × 106B. pertussis genomes/mL and showed no false-positives. Rapid (< 20 min) LFIA detection of TcfA as a biomarker for B. pertussis infection is feasible and may facilitate early detection of pertussis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis

Burnham-Marusich

Olsen

Scarbrough

et al. 2020

Sci Rep

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“…Bordetella holmesii strain ATCC 51541 (DSM 13416) was obtained from DSMZ collection (Leibniz-Institut, Berlin, Germany). B. pertussis strains 186 and 606, used in the current wP vaccine manufactured in Poland, were acquired from the National Medicines Institute (Warsaw, Poland) [ 30 ]. Bordetella parapertussis PCM 529 (ATCC 15311), Bordetella bronchiseptica PCM 530 (ATCC 19395), and Bordetella bronchiseptica PCM 1943 (ATCC 4617) came from the PCM collection (Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland).…”

Section: Methodsmentioning

confidence: 99%

Bordetella holmesii Lipopolysaccharide Hide and Seek Game with Pertussis: Structural Analysis of the O-Specific Polysaccharide and the Core Oligosaccharide of the Type Strain ATCC 51541

Ucieklak

Koj

Niedziela

2020

IJMS

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Whooping cough is a highly contagious disease caused predominantly by Bordetella pertussis, but it also comprises of a pertussis-like illness caused by B. holmesii. The virulence factors of B. holmesii and their role in the pathogenesis remain unknown. Lipopolysaccharide is the main surface antigen of all Bordetellae. Data on the structural features of the lipopolysaccharide (LPS) of B. holmesii are scarce. The poly- and oligosaccharide components released by mild acidic hydrolysis of the LPS were separated and investigated by 1H and 13C NMR spectroscopy, mass spectrometry, and chemical methods. The structures of the O-specific polysaccharide and the core oligosaccharide of B. holmesii ATCC 51541 have been identified for the first time. The novel pentasaccharide repeating unit of the B. holmesii O-specific polysaccharide has the following structure: {→2)-α-l-Rhap-(1→6)-α-d-Glcp-(1→4)-[β-d-GlcpNAc-(1→3]-α-d-Galp-(1→3)-α-d-GlcpNAc-(1→}n. The SDS-PAGE and serological cross-reactivities of the B. holmesii LPS suggested the similarity between the core oligosaccharides of B. holmesii ATCC 51541 and B. pertussis strain 606. The main oligosaccharide fraction contained a nonasaccharide. The comparative analysis of the NMR spectra of B. holmesii core oligosaccharide fraction with this of the B. pertussis strain 606 indicated that the investigated core oligosaccharides were identical.

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“…Przeprowadzone badania molekularne wykazały, że brak syntezy pertaktyny nie był wynikiem ekspansji pojedynczego klonu lecz był skutkiem powstania niezależnych mutacji. Opisano szereg mechanizmów takich jak: insercja IS481 lub IS1002 w gen kodujący Prn, delecja całego genu prn lub jego fragmentów, pojawienie się kodonu STOP w miejscu powodującym przedwczesne zakończenie syntezy białka, insercja guaniny do homopolimerycznego fragmentu poly(G) prowadząca do odwracalnego przesunięcia ramki odczytu w mechanizmie zmienności fazowej, inwersja części promotora prn, tranzycja G → A w pozycji -162 uniemożliwiająca transkrypcję genu prn, a także dotychczas niezidentyfikowane mutacje występujące poza promotorem oraz rejonem kodującym genu prn [76].…”

Section: Bordetella Pertussisunclassified

Causes Of Pertussis Incidence Increase

Prygiel¹,

Mosiej²,

Zasada³

2020

Postępy Mikrobiologii - Advancements of Microbiology

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Pertactin-deficient Bordetella pertussis isolates in Poland—a country with whole-cell pertussis primary vaccination

Cited by 19 publications

References 43 publications

Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis

Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis

Bordetella holmesii Lipopolysaccharide Hide and Seek Game with Pertussis: Structural Analysis of the O-Specific Polysaccharide and the Core Oligosaccharide of the Type Strain ATCC 51541

Causes Of Pertussis Incidence Increase

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