Perspectives on the synthesis and use of ageladine A

Mordhorst, Thorsten; Bickmeyer, Ulf

doi:10.1016/j.tetlet.2015.05.081

Cited by 12 publications

(6 citation statements)

References 29 publications

(27 reference statements)

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“…chuii . This difference in peak position between the algal species as well as its deviance from the characteristics of Ag A in water, may be due to the intracellular conditions such as differences in pH [ 19 ]. The only eukaryote which did not exhibit a local maximum in this wavelength range was C .…”

Section: Discussionmentioning

confidence: 99%

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Sponge-derived Ageladine A affects the in vivo fluorescence emission spectra of microalgae

et al. 2020

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In several marine hosts of microalgae, fluorescent natural products may play an important role. While the ecological function of these compounds is not well understood, an interaction of these molecules with the photosynthesis of the symbionts has been suggested. In this study, the effect of Ageladine A (Ag A), a pH-dependent fluorophore found in sponges of the genus Agelas, on microalgal fluorescence was examined. The spectra showed an accumulation of Ag A within the cells, but with variable impacts on fluorescence. While in two Synechococcus strains, fluorescence of phycoerythrin increased significantly, the fluorescence of other Synechococcus strains was not affected. In four out of the five eukaryote species examined, chlorophyll a (Chl a) fluorescence intensity was modulated. In Tisochrysis lutea, for example, the position of the fluorescence emission maximum of Chl a was shifted. The variety of these effects of Ag A on microalgal fluorescence suggests that fluorophores derived from animals could play a crucial role in shaping the composition of marine host/symbiont systems.

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Section: Discussionmentioning

confidence: 99%

“…The peak in excitation wavelength is at 370 nm with the emission ranging from 415 nm to 500 nm with a maximum at 415 nm [ 18 ], both measured in water. The excitation/emission profile changes with the environment [ 19 ]. Its specific function within the sponge holobiont, has not been identified.…”

Section: Introductionmentioning

confidence: 99%

Sponge-derived Ageladine A affects the in vivo fluorescence emission spectra of microalgae

et al. 2020

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“…Experiments were performed at room temperature (22 ± 1.5°C) Ageladine A used was synthetized by Thorsten Koschmieder (nee Mordhorst) at the Alfred Wegener Institute in Bremerhaven [ 11 ]. Preliminary tests revealed no influence of the fluorescent dye Ageladine A on the response of the oxygen optodes.…”

Section: Methodsmentioning

confidence: 99%

“…The widely distributed sponges of the genus Agelas ( Fig 1a ) [ 6 ] produce the fluorescing pyrrole imidazole alkaloid Ageladine A. This compound was first detected and described during the search for an antiangiogenic matrixmetalloproteinases inhibitor [ 7 ] and later chemically synthetized [ 8 – 11 ]. Ageladine A [ 7 ] accumulates in acidic cellular compartments [ 12 ] and has previously been used as a pH sensitive, fluorescent dye [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of photosynthesis in Synechococcus bacillaris by sponge-derived Ageladine A

et al. 2019

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This study is a proof of concept that the sponge derived pyrrole-imidazole alkaloid Ageladine A acts as an additional light harvesting molecule for photosynthesis of symbionts of marine sponges. The absorbance of Ageladine A is in the UV range and fluoresces blue, matching the blue absorbance of chlorophyll a. A joint modeling and experimental approach demonstrates that Ageladine A increases photosynthetic O2 production of Synechococcus bacillaris WH5701 (CCMP1333), when the cells are exposed to UV light, which is marginally used for photosynthesis. Due to the presence of Ageladine A, production of O2 increased 2.54 and 3.1-fold, in the experiments and the model, respectively.

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“…This shift in molecular composition corresponds to an increase in fluorescence, which peaks at pH 3–4. The peak in excitation wavelength in water at 370 nm corresponds to an emission ranging from 415 nm to 500 nm with a maximum at 415 nm [ 2 ], however these excitation/emission profiles can change with chnaging environments [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of sponge-derived Ageladine A on the photosynthesis of different microalgal species and strains

et al. 2020

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Fluorescent natural compounds have been identified in several marine hosts of microalgae. Their prevalence, and the energy the host is expending on their synthesis, suggests an important, yet poorly understood ecological role. It has been suggested that some of these natural products may enhance the photosynthesis of microbial symbionts. In this study, the effect of Ageladine A (Ag A), a pH-dependent fluorophore found in sponges of the genus Agelas, on the photosynthesis of nine microalgal species and strains was examined. The data showed that the variety of effects of Ag A additions differed between species, and even strains within a species. While in one strain of Synechococcus sp., the presence of Ag A increased gross photosynthesis under UV light exposure, it decreased in another. And while in the chlorophyte T. chuii overall metabolic activity was greatly reduced under all forms of lighting, photosynthesis in T. lutea was positively affected by the addition of Ag A. The variety of effects of Ag A on photosynthesis observed in this study indicate a complex interaction of Ag A with microalgal cells and suggests that a host may be able to shape its own symbiotic microbiome with self-produced natural products.

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Perspectives on the synthesis and use of ageladine A

Cited by 12 publications

References 29 publications

Sponge-derived Ageladine A affects the in vivo fluorescence emission spectra of microalgae

Sponge-derived Ageladine A affects the in vivo fluorescence emission spectra of microalgae

Enhancement of photosynthesis in Synechococcus bacillaris by sponge-derived Ageladine A

Effects of sponge-derived Ageladine A on the photosynthesis of different microalgal species and strains

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