Perspectives on flow biocatalysis: the engine propelling enzymatic reactions

Abstract

Flow biocatalysis has emerged as an empowering tool to boost the potential of enzymatic reactions towards more automatized, sustainable, and generally efficient synthetic processes. In the last fifteen years, the increasing number of biocatalytic transformations carried out in continuous flow exemplified the benefits that this technology can bring to incorporate biocatalysis into industrial operations. This perspective aims to capture in a nutshell the available methodologies for flow biocatalysis as well as to discuss the current limitations and the future directions in this field. Graphical abstract

Biocatalytic Synthesis of l‐Pipecolic Acid by a Lysine Cyclodeaminase: Batch and Flow Reactors

“…[38] To sum up, we would like to highlight that the choice of biocatalyst and type of reactor must be carefully evaluated according to the desired output as different biotechnological approaches can be successfully implemented offering different advantages. [39,40] Experimental Section Materials All the chemicals were purchased from Sigma Aldrich (Gillingham, UK), Thermo Fisher (Loughborough, UK), Acros Organics (Reinach, Switzerland), and Apollo Scientific and used without further purification. The methacrylate supports were acquired from Resindion S.R.L.…”

Biocatalysis in microfluidic systems: an experimental basis for data science

“…Some excellent examples are reported in two recent reviews. 42,43 It is also clear that using such reactor systems, monitoring and control (of conditions) becomes easier since operation is (mostly) in steady-state. 44 The need for mathematical modelling based on a theoretical understanding of hydrodynamics and reaction phenomena is also of great importance.…”