Perspectives in Super-Resolved Fluorescence Microscopy: What Comes Next?

Tip‐Enhanced Raman Spectroscopy: A Tool for Nanoscale Chemical and Structural Characterization of Biomolecules

“…Among them, the most popular is probably fluorescence microscopy, owing to the high emission cross section (≈10 −16 cm 2 sr −1 ), which allows the study of small structures and processes in living cells and tissues. Although the spatial optical resolution (SOR) is then limited by diffraction to a few hundreds of nanometers, it can be significantly improved and reach a value as low as about 5 nm by means of super‐resolved fluorescence microscopy techniques, such as stimulated emission depletion (STED), stochastic optical reconstruction microscopy (STORM), or photoactivated localization microscopy (PALM) . However, fluorescent labeling is required, which alters the molecular specificity of the analysis, and necessarily high light doses may inflict damage on the sample.…”

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

“…An intensively developing group of SRM methods opens new perspectives in this field [149]. Theoretically, SRM may achieve a resolution of up to 1 nm: therefore, it is a unique tool to be used to study processes on a scale ranging from an individual molecule to a whole cell.…”

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

“…The topicality of light microscopy for the investigation of intranuclear processes has always been high. An intensively developing group of SRM methods opens new perspectives in this field [ 149 ]. Theoretically, SRM may achieve a resolution of up to 1 nm: therefore, it is a unique tool to be used to study processes on a scale ranging from an individual molecule to a whole cell.…”