Perspectives for Diagnosis and Control of Leishmaniasis Based on Volatile Organic Compounds

Andrade, Jaílson Bittencourt de; Magalhes, Jairo Torres; Mesquita, Paulo Roberto Ribeiro; Santos, Fbio; Medeiros, Frederico de; Maria, Stella

doi:10.5772/57279

Cited by 2 publications

(1 citation statement)

References 156 publications

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“…Various metabolomic methods, used to measure the abundance or flux of metabolites, or small molecule metabolites in a biological system, have been employed to provide insight into the complex molecular interactions that underpin the proliferation of vector-borne pathogens 9 10 11 12 13 , but similar efforts have been scarce for Lyme disease spirochetes 14 15 . A wide variety of instruments are employed for metabolomics projects, often utilizing a particular metabolite extraction solvent system 16 followed by a chromatographic separation scheme (gas chromatography (GC), liquid chromatography (LC), hydrophilic interaction (HILIC)) coupled with mass spectrometry (MS) 17 .…”

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Metabolomics of the tick-Borrelia interaction during the nymphal tick blood meal

Hoxmeier

Fleshman

Broeckling

et al. 2017

Sci Rep

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The causal agents of Lyme disease in North America, Borrelia burgdorferi and Borrelia mayonii, are transmitted primarily by Ixodes scapularis ticks. Due to their limited metabolic capacity, spirochetes rely on the tick blood meal for nutrients and metabolic intermediates while residing in the tick vector, competing with the tick for nutrients in the blood meal. Metabolomics is an effective methodology to explore dynamics of spirochete survival and multiplication in tick vectors before transmission to a vertebrate host via tick saliva. Using gas chromatography coupled to mass spectrometry, we identified statistically significant differences in the metabolic profile among uninfected I. scapularis nymphal ticks, B. burgdorferi-infected nymphal ticks and B. mayonii-infected nymphal ticks by measuring metabolism every 24 hours over the course of their up to 96 hour blood meals. Specifically, differences in the abundance of purines, amino acids, carbohydrates, and fatty acids during the blood meal among the three groups of nymphal ticks suggest that B. mayonii and B. burgdorferi may have different metabolic capabilities, especially during later stages of nymphal feeding. Understanding mechanisms underlying variable metabolic requirements of different Lyme disease spirochetes within tick vectors could potentially aid development of novel methods to control spirochete transmission.

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confidence: 99%

Metabolomics of the tick-Borrelia interaction during the nymphal tick blood meal

Hoxmeier

Fleshman

Broeckling

et al. 2017

Sci Rep

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Metabolomic Bioinformatic Analysis

Dailey

2017

Methods in Molecular Biology

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Metabolomics allows for the investigation of the small molecules found within living systems. Based on the design of the experiments, it is not uncommon for these analyses to include matrices of thousands of variables. In order to handle such large datasets, many have turned to multivariate statistical analyses to analyze and understand their data. Herein, we present protocols for using R to analyze metabolomic data using some of the more common multivariate statistical techniques.

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Perspectives for Diagnosis and Control of Leishmaniasis Based on Volatile Organic Compounds

Cited by 2 publications

References 156 publications

Metabolomics of the tick-Borrelia interaction during the nymphal tick blood meal

Metabolomics of the tick-Borrelia interaction during the nymphal tick blood meal

Metabolomic Bioinformatic Analysis

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