Perspectivas do uso de fitoterápicos no tratamento do sêmen caprino infectado pelo vírus da Artrite Encefalite Caprina

Peixoto, Renato Mesquita; Teixeira, Maria Fátima da Silva; Andrioli, A.; Pinheiro, R. R.; Silva, Antonio Adailson de Sousa; Aguiar, Tereza D'ávila de Freitas; Azevedo, Dalva Alana Aragão de; Sousa, A. L. M. de

doi:10.26605/medvet-v12n3-2395

Cited by 3 publications

(1 citation statement)

References 52 publications

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“…Much research has been conducted on bioactive substances to investigate their possible antiviral effects against infections in humans and animals. In this context, plants from several botanical families, and their parts, such as the leaves, roots, flowers, and seeds, have been studied and revealed to possess promising substances in their composition 27 .…”

Section: Discussionmentioning

confidence: 99%

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

Sousa

Pinheiro

Araújo³

et al. 2023

Sci Rep

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This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum.

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Section: Discussionmentioning

confidence: 99%

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

Sousa

Pinheiro

Araújo³

et al. 2023

Sci Rep

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In vivo evaluation of the antiretroviral activity of Melia azedarach against small ruminant lentiviruses in goat colostrum and milk

Souza,

Pinheiro,

Peixoto

et al. 2023

Braz J Microbiol

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Immune response dynamics of recent and chronic small ruminant lentivirus infection in the male reproductive system

Peixoto¹,

Pinheiro

et al. 2023

Sem. Ci. Agr.

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The objective of this study was to analyze the immune responses of bucks to small ruminant lentivirus (SRLV) with a focus on the reproductive system of males with recent and chronic infection. A total of 12 bucks were selected, six seronegative and six seropositive with chronic natural infection for more than 18 months (chronic infection group). After selecting the animals, the six seronegative males were intravenously inoculated with caprine arthritis-encephalitis virus (CAEV)-Co viral strain at a titer of 10-5,6 TCID50/mL. After viral inoculation, this group was called the recent infection group and was monitored weekly with the chronically infected group for 180 days with blood serum and seminal plasma Western Blot (WB) analysis. Of the animals with chronic SRLV infection, 18.94% (50/264) showed anti-SRLV antibodies in at least one of the samples, and 81.06% (214/264) were negative. Anti-SRLV antibodies were detected in 27.27% (36/132) of the blood serum samples from this group, while 10.60% (14/132) were reactive in the seminal plasma WB test. The animals inoculated with CAEV-Co became seropositive after the third week of viral inoculation. In this group, 31.06% (41/132) of seminal plasma samples had anti-SRLV antibodies, and of these, 70.73% (29/41) coincided with blood serum results. Of the remaining 29.27% (12/41), the seminal plasma sample of only three animals (RIA2, RIA3, and RIA5) had anti-SRLV antibodies. One of the animals with a recent infection presented anti-SRLV antibodies only in seminal plasma samples, possibly due to virus compartmentalization. Intermittent viral shedding was observed in both biological samples, regardless of the infection stage. The immune response in bucks with recent SRLV infection is more significant than in chronically infected animals. Regardless of the stage of infection, there is a fluctuation in antibody levels, therefore, this creates a risk of false-negative samples when performing the diagnosis.

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Perspectivas do uso de fitoterápicos no tratamento do sêmen caprino infectado pelo vírus da Artrite Encefalite Caprina

Cited by 3 publications

References 52 publications

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

In vivo evaluation of the antiretroviral activity of Melia azedarach against small ruminant lentiviruses in goat colostrum and milk

Immune response dynamics of recent and chronic small ruminant lentivirus infection in the male reproductive system

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