Personalised medicine in veterinary oncology: One to cure just one

Klopfleisch, Robert

doi:10.1016/j.tvjl.2015.01.004

Cited by 20 publications

(16 citation statements)

References 75 publications

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“…Combining molecular information with histopathology and clinical staging, individual treatment strategies can be designed for maximum effectiveness. 5,14 Indeed, gene expression studies are accepted in human oncology as a validated method to discover biomarkers of disease and determine treatment options for example in lymphoma, 15 breast, 16 bladder cancers, 17 sarcomas, 18 and other tumor types. 19 With the sequencing of the canine genome, similar biomarker strategies can be developed for veterinary diagnostics.…”

Section: Discussionmentioning

confidence: 99%

“…Thus far, limited molecular studies in companion animals have demonstrated the utility of examining gene signatures of tumors to discover potential biomarkers of outcome and treatment. [1][2][3][4][5] However, in clinical veterinary practice use of genomic information for supportive diagnostics remains unfulfilled. A complimentary approach using gene expression profiling is highly useful in studies of human cancers and can be extrapolated to cancers in dogs.…”

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confidence: 99%

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Validation of a Multiplexed Gene Signature Assay for Diagnosis of Canine Cancers from Formalin‐Fixed Paraffin‐Embedded Tissues

Davis¹,

Schwartz

Duchemin³

et al. 2017

Veterinary Internal Medicne

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BackgroundUse of molecular‐based diagnostics for companion animals is impeded by availability of technology platforms, tissue acquisition requirements, and species‐specific reagents.Hypothesis/ObjectivesTo validate a quantitative nuclease protection assay (qNPA) to simultaneously measure RNA expression of multiple genes in archived formalin‐fixed paraffin‐embedded (FFPE) tumors from dogs.AnimalsAll tumor biopsy samples were collected retrospectively from surgical biopsies and in the care of veterinarians.MethodsRetrospective case series. A qNPA 96‐well ArrayPlate was built using 30 canine‐specific genes, 5 housekeeping genes, positive and negative controls with qualified gene‐specific oligonucleotides. Pearson's correlation, coefficient of variation (CV), and multivariate analysis were used to determine analytical performance using 40 FFPE dog tumors. Once validated, 70 FFPE dog tumors were analyzed for differences in gene expression using hierarchical clustering and analysis of variance of log transformed data. Immunohistochemistry (IHC) was performed to correlate gene expression and protein expression in a subset of tumors.ResultsThe assay was linear with decreasing sample input (R 2 = 0.978), reproducible within and between 96‐well plates (r = 0.988 and 0.95, respectively) and between different laboratories (CV = 0.96). Hierarchical cluster analysis showed grouping of tumors by histogenesis and oncogenes. Significant differences were found between BCl2, E2F transcription factor 1, MDM2, COX‐2, MET proto‐oncogene receptor kinase, and other biologically relevant gene expression in tumor subtypes. Immunohistochemistry confirmed protein expression.Conclusions and Clinical ImplicationsBecause this technology works reliably on FFPE specimens, it can help expedite the broad introduction of multiplexed genomic information for improved diagnostics and discovery of new targets for therapies in veterinary oncology.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Validation of a Multiplexed Gene Signature Assay for Diagnosis of Canine Cancers from Formalin‐Fixed Paraffin‐Embedded Tissues

Davis¹,

Schwartz

Duchemin³

et al. 2017

Veterinary Internal Medicne

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“…Effective therapy and positive prognosis rely on early diagnosis and adequate classification of cancer type, providing information necessary for targeted therapies. 10 In human medicine, the application of highthroughput omics has been proven useful to investigate cancerogenesis. In canine oncology, on the contrary, the application of omics techniques is still far behind.…”

Section: P Roccabiancamentioning

confidence: 99%

“…Although the perspective of the evaluation of the single patient in veterinary oncology routine practice might appear somehow futuristic in veterinary medicine, recent studies have introduced the concept of personalised medicine in veterinary therapy as well. 10,67,104,140 Therefore, the application of omics and system biology disciplines in veterinary routine practices does not seem that far anymore. …”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Application of post-genomic techniques in dog cancer research

et al. 2016

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Omics techniques have been widely applied to veterinary science, although mostly on farm animal productions and infectious diseases. In canine oncology, on the contrary, the use of omics methodologies is still far behind. This review presents the most recent achievement in the application of postgenomic techniques, such as transcriptomics, proteomics, and metabolomics, to canine cancer research. The protocols to recover material suitable for omics analyses from formalin-fixed, paraffinembedded tissues are presented, and omics applications for biomarker discovery and their potential for cancer diagnostics in veterinary medicine are highlighted.

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“…Studies like the one described by Klopfleisch (2015) are closing these gaps and identifying novel prognostic markers and therapeutic targets. Studies like the one described by Klopfleisch (2015) are closing these gaps and identifying novel prognostic markers and therapeutic targets.…”

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confidence: 99%

Transcriptional profiling of canine mast cell tumors: Searching for candidate targets and prognostic markers

Webster¹

2016

The Veterinary Journal

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Personalised medicine in veterinary oncology: One to cure just one

Cited by 20 publications

References 75 publications

Validation of a Multiplexed Gene Signature Assay for Diagnosis of Canine Cancers from Formalin‐Fixed Paraffin‐Embedded Tissues

Validation of a Multiplexed Gene Signature Assay for Diagnosis of Canine Cancers from Formalin‐Fixed Paraffin‐Embedded Tissues

Application of post-genomic techniques in dog cancer research

Transcriptional profiling of canine mast cell tumors: Searching for candidate targets and prognostic markers

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