Personal Glucose Meter for α-Glucosidase Inhibitor Screening Based on the Hydrolysis of Maltose

Tian, Tao; Chen, Guoying; Zhang, Hao; Yang, Feng‐Qing

doi:10.3390/molecules26154638

Cited by 16 publications

(10 citation statements)

References 24 publications

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“…Thus, the inhibition of α-glucosidase activity can slow down glucose production and absorption, and then reduce postprandial blood glucose. The molecular docking study can be used to predict the binding sites and modes of small-molecule compounds to α-glucosidase [19]. Oxidative stress occurs when the production of free radicals exceeds the cell's inherent antioxidant defense system, which plays an important role in the occurrence and development of diabetes and its complications [20].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, Hypoglycemic and Molecular Docking Studies of Methanolic Extract, Fractions and Isolated Compounds from Aerial Parts of Cymbopogon citratus (DC.) Stapf

Wang

Zhang

et al. 2022

Molecules

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Traditionally, Cymbopogon citratus is used to treat a variety of ailments, including cough, indigestion, fever, and diabetes. The previous chemical and bioactive research on C. citratus mainly focused on its volatile oil. In this study, 20 non-volatile known compounds were isolated from the dried aerial part of C. citratus, and their structures were elucidated by MS, NMR spectroscopy, and comparison with the published spectroscopic data. Among them, 16 compounds were reported for the first time from this plant. The screening results for antioxidant and α-glucosidase inhibitory activities indicated that compounds caffeic acid (5), 1-O-p-coumaroyl-3-O-caffeoylglycerol (8), 1,3-O-dicaffeoylglycerol (9) and luteolin-7-O-β-D-glucopyranoside (12) had potent antioxidant capacities, with IC50 values from 7.28 to 14.81 μM, 1.70 to 2.15 mol Trolox/mol and 1.31 to 2.42 mol Trolox/mol for DPPH, ABTS, and FRAP, respectively. Meanwhile, compounds 8 and 9 also exhibited significant inhibitory activities against α-glucosidase, with IC50 values of 11.45 ± 1.82 μM and 5.46 ± 0.25 μM, respectively, which were reported for the first time for their α-glucosidase inhibitory activities. The molecular docking result provided a molecular comprehension of the interaction between compounds (8 and 9) and α-glucosidase. The significant antioxidant and α-glucosidase inhibitory activities of compounds 8 and 9 suggested that they could be developed into antidiabetic drugs because of their potential regulatory roles on oxidative stress and digestive enzyme.

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Section: Introductionmentioning

confidence: 99%

Antioxidant, Hypoglycemic and Molecular Docking Studies of Methanolic Extract, Fractions and Isolated Compounds from Aerial Parts of Cymbopogon citratus (DC.) Stapf

Wang

Zhang

et al. 2022

Molecules

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“…First of all, the antioxidant/oxidant potential of biomaterials to be used for industrial purposes must be proven with many different parameters in vitro / in vivo and clinical trials. In recent years, new enzymes with antioxidant enzyme‐like activity have made significant progress with their increasing applications in therapeutic, diagnostic medicine, biosensing and immuno‐analysis [34–36] . On the other hand, nanoparticles must be handled with care due to their potential toxicities [37] Nanomaterials can be toxic due to their small surface area, solid particle framework, type of metal ion and organic component used.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, new enzymes with antioxidant enzyme-like activity have made significant progress with their increasing applications in therapeutic, diagnostic medicine, biosensing and immunoanalysis. [34][35][36] On the other hand, nanoparticles must be handled with care due to their potential toxicities [37] Nanomaterials can be toxic due to their small surface area, solid particle framework, type of metal ion and organic component used. In addition, the concentrations and morphologies of nanomaterials are also associated with toxicity.…”

Section: Reusability Of the β-Gnfmentioning

confidence: 99%

Glucose Tolerance, Antiprotease Activity and Total Oxidant/Antioxidant Capacity Studies of β‐Glucosidase Hybrid Nanoflower for Industrial Applications

Gülmez¹

2022

Chemistry & Biodiversity

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β‐Glycosidases, which catalyse the hydrolysis of glycoside bonds, have a wide spectrum of industrial applications. However, the reaction product glucose inhibits the activities of many β‐glucosidases. Consequently, the reduced catalytic activities of the enzyme limit the industrial applications of the enzymes. For that reason, the studies dealing with maintaining the activities of the relevant enzymes at high glucose concentrations are a great interest among the researchers. In this context, herein, protein‐inorganic hybrid nanoflowers were synthesized using β‐glucosidase and copper ion by fast sonication method for 10 min. After characterization of synthesized nanoflowers, pH/temperature studies, glucose tolerance, anti‐protease activity, recyclability and total antioxidant and total oxidative capacity levels were estimated. Accordingly, the optimum pHs of free β‐glucosidase and hybrid nanoflower (β‐GNF) were found to be 6 and 5, respectively, and the optimum temperature values for both hybrid nanoflowers and free enzyme were 40 °C. β‐GNF exhibited better activity than free enzyme in low acidic and alkaline environment and at high temperature. The nanoflower retained nearly all (99 %) of its initial activity at all glucose concentrations (0.01, 0.05 and 0.1 mg/mL), especially at pH 5 and 6. Also, β‐GNF maintained more than 90 % of initial activity at 0.01 and 0.05 mg/mL glucose at pH 4 and 7. It also displayed about 96 % high residual activity after proteinase K treatment for 3 h at 37 °C, while that of the free β‐glucosidase was about 87 %. The reusability studies showed that β‐GNF only lost ∼28 % of its initial activities at the end of five cycles. The hybrid nanoflowers at 5 mg/mL concentration exhibited the high total antioxidant capacity. In addition, low total oxidant capacity and oxidative stress index levels were recorded at the same concentration of the hybrid nanoflower. The findings of the present study revealed that β‐GNFs may be evaluated as a candidate for various industrial applications due to its high glucose tolerance, anti‐protease activity, reusability and resistance to low acidic/alkaline environment and high temperature.

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“…Diabetes mellitus (DM), associated with carbohydrate metabolism disorders and characterized by high blood glucose levels, has become a severe global non-communicable disease [1]. With 463 million people around the world suffering from DM, it is one of the top ten causes of death globally.…”

Section: Introductionmentioning

confidence: 99%

“…With 463 million people around the world suffering from DM, it is one of the top ten causes of death globally. DM causes about 1.5 million deaths per year worldwide [1,2]. It is estimated that the total number of individuals with diabetes worldwide will increase to 700 million by 2045.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Fisetin on α-Glucosidase Activity: Kinetic and Molecular Docking Studies

et al. 2021

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The inhibition of α-glucosidase is a clinical strategy for the treatment of type 2 diabetes mellitus (T2DM), and many natural plant ingredients have been reported to be effective in alleviating hyperglycemia by inhibiting α-glucosidase. In this study, the α-glucosidase inhibitory activity of fisetin extracted from Cotinus coggygria Scop. was evaluated in vitro. The results showed that fisetin exhibited strong inhibitory activity with an IC50 value of 4.099 × 10−4 mM. Enzyme kinetic analysis revealed that fisetin is a non-competitive inhibitor of α-glucosidase, with an inhibition constant value of 0.01065 ± 0.003255 mM. Moreover, fluorescence spectrometric measurements indicated the presence of only one binding site between fisetin and α-glucosidase, with a binding constant (lgKa) of 5.896 L·mol−1. Further molecular docking studies were performed to evaluate the interaction of fisetin with several residues close to the inactive site of α-glucosidase. These studies showed that the structure of the complex was maintained by Pi-Sigma and Pi-Pi stacked interactions. These findings illustrate that fisetin extracted from Cotinus coggygria Scop. is a promising therapeutic agent for the treatment of T2DM.

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Personal Glucose Meter for α-Glucosidase Inhibitor Screening Based on the Hydrolysis of Maltose

Cited by 16 publications

References 24 publications

Antioxidant, Hypoglycemic and Molecular Docking Studies of Methanolic Extract, Fractions and Isolated Compounds from Aerial Parts of Cymbopogon citratus (DC.) Stapf

Antioxidant, Hypoglycemic and Molecular Docking Studies of Methanolic Extract, Fractions and Isolated Compounds from Aerial Parts of Cymbopogon citratus (DC.) Stapf

Glucose Tolerance, Antiprotease Activity and Total Oxidant/Antioxidant Capacity Studies of β‐Glucosidase Hybrid Nanoflower for Industrial Applications

Inhibitory Effect of Fisetin on α-Glucosidase Activity: Kinetic and Molecular Docking Studies

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