Persisting lung pathogenesis and minimum residual virus in hamster after acute COVID-19

A Heterologous Challenge Rescues the Attenuated Immunogenicity of SARS-CoV-2 Omicron BA.1 Variant in Syrian Hamster Model

“…Asymptomatic people may carry and shed virus for transmission. Unfortunately, persistent symptoms and sequelae are usually observed in the discharged patients ( 3 – 5 ) and are also demonstrated in experimental animals that recovered from COVID-19 ( 6 ). Currently approved vaccines are playing important roles in mitigating the ongoing pandemic and reducing SARS-CoV-2-caused deaths.…”

Dexamethasone ameliorates severe pneumonia but slightly enhances viral replication in the lungs of SARS-CoV-2-infected Syrian hamsters

“…To mimic patients with severe pneumonia caused by SARS-CoV-2, Syrian hamsters were intranasally infected with 1 × 10 4 plaque-forming units (PFUs) of an ancestral SARS-CoV-2 strain (AP-8) as previously described [ 7 , 8 ]. SARS-CoV-2-infected hamsters were untreated or treated with 1, 3, or 5 doses of dexamethasone (1 mg/kg per dose) via intraperitoneal injection (Fig.…”

Antiviral Nanobiologic Therapy Remodulates Innate Immune Responses to Highly Pathogenic Coronavirus

“…The hamsters were intranasally inoculated with 1 × 10 4 plaque‐forming units (PFU) of prototype SARS‐CoV‐2 as previously described. [ 12 ] The hamsters without infection were set as a control group. Five days after infection (5 dpi), all hamsters were euthanized for immunological and virological examinations.…”

“…Neutralization Assay of Authentic Virus System: Neutralization assay of authentic SARS-CoV-2 viruses was measured by a standard median tissue culture infective dose (TCID 50 ) method in Vero cells seeded in a 96-well plate as previously described. [12] Samples were serially diluted and incubated with 100 TCID 50 of SARS-CoV-2 for 1 h. And then, the mix was added S2, Supporting Information). Viral RNA levels in G) turbinate, H) trachea and I) lung tissues collected at 5 dpi were measured by RT-PCR (n = 5).…”