Persistent oligoclonal CD4^dimCD8⁺T cells in peripheral blood

Abstract

Background Routine CD4/CD8 T‐cell phenotyping may shows a small fraction of CD4dimCD8+ T cells with a homogeneous appearance as described for lymphoproliferative syndromes or chronic infections. The aim of this study was to elucidate the significance of CD4dimCD8+ T cells and their degree of diversity. Methods Phenotyping was performed in 272 samples from healthy donors, elderly patients, and immunocompromised (human immunodeficiency virus or renal transplantation) patients. Results The CD4dimCD8+ T cells had decreased fluorescence intensity for CD4 but not for CD8. The frequency of patients with CD4dimCD8+ T cells (>20 cells/μl; 10.3% of patients with human immunodeficiency virus and 7.7% with renal transplantation) was not significantly different when compared with healthy donors (9.7%). The CD4dimCD8+ T cells did not express the activation marker CD69. The CD8 of CD4dimCD8+ T cells expressed the heterodimeric (β) isoform. In 13 of 26 samples, the apparently highly homogeneous CD4dimCD8+ T cells expressed one predominant T‐cell receptor Vβ clonotype. These predominant clonotypes were widely distributed among patients: Vβ 5.2, 17, 2, 3, 5.1, 13.1, 14, and 20. Conclusions Whether these findings demonstrate an oligoclonal reaction to chronic inflammation or an emerging lymphoproliferative disorder must be elucidated in a long‐term longitudinal study. By analogy to monoclonal gammopathy, we propose to name this phenomenon “oligoclonal clonopathy of undetermined significance.” © 2005 Wiley‐Liss, Inc.

The characteristics of immune system changes in children who ingested melamine-contaminated powdered formula in China

“…In the group aged 3-5 years old, the concentration of CD3 þ , CD4 þ and CD8 þ had no difference between children with stones and children without stones. CD4 þ /CD8 þ lymphocyte phenotyping is the most frequently performed analysis in flow cytometry for monitoring cellular immunology (Lambert 2005). Thus, we calculated the CD4 þ /CD8 þ ratio between children with stones and children without stones in each group.…”

Spectrum of Clonal Large Granular Lymphocytes (LGLs) of αβ T Cells

“…Most expressed CD8 without CD4, but a significant number coexpressed CD4 and CD8, as has been previously described. [23][24][25] LGLT clones have also been reported previously. 26,27 Most of these small LGLT clones did not change the clinical management, but there may be patients who need treatment for an LGLT clone because of its immunologic effects, such as cytopenias, even if the clone size were small.…”

Enumeration of peripheral lymphocyte subsets using 6 vs. 4 color staining: A clinical evaluation of a new flowcytometer

“…Our and previous works (9)(10)(11)(12)(13)(14)(15)(16) show additional T cell subsets at highly variable levels with probable clinical relevance that have to be clarified. The CD4 þ CD8dim T cells are most probably derived from the CD4 þ T cells but express the CD8aa homodimeric isoform suggesting they could be related to the mucosal immunity (9,10; personal data) with preferentially a Th1 activity (28).…”